Molecular analysis of the Sydney rock oyster (Saccostrea glomerata) CO2 stress response
Background: Human activities have led to a substantial increase in carbon dioxide (CO2) emission, with further increases predicted. A RNA-Seq study on adult Saccostrea glomerata was carried out to examine the molecular response of this bivalve species to elevated pCO2. Results: A total of 1626 S. gl...
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ftunivscoast:usc:20697 2023-05-15T17:51:50+02:00 Molecular analysis of the Sydney rock oyster (Saccostrea glomerata) CO2 stress response Ertl, Nicole G O'Connor, W A Wiegand, A Elizur, A 2016 https://doi.org/10.1186/s40665-016-0019-y eng eng BioMed Central Ltd. usc:20697 URN:ISSN: 2053-7565 Copyright © 2016 The Author(s). Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver(http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated CC0 PDM CC-BY FoR 0608 (Zoology) FoR 0601 (Biochemistry and Cell Biology) FoR 0604 (Genetics) saccostrea glomerata Sydney rock oyster molluscs RNA-seq stress carbon dioxide immunity biomineralisation Journal Article 2016 ftunivscoast https://doi.org/10.1186/s40665-016-0019-y 2020-03-23T23:28:28Z Background: Human activities have led to a substantial increase in carbon dioxide (CO2) emission, with further increases predicted. A RNA-Seq study on adult Saccostrea glomerata was carried out to examine the molecular response of this bivalve species to elevated pCO2. Results: A total of 1626 S. glomerata transcripts were found to be differentially expressed in oysters exposed to elevated pCO2 when compared to control oysters. These transcripts cover a range of functions, from immunity (e.g. pattern recognition receptors, antimicrobial peptides), to respiration (e.g. antioxidants, mitochondrial respiratory chain proteins) and biomineralisation (e.g. carbonic anhydrase). Overall, elevated levels of CO2 appear to have resulted in a priming of the immune system and in producing countermeasures to potential oxidative stress. CO2 exposure also seems to have resulted in an increase in the expression of proteins involved in protein synthesis, whereas transcripts putatively coding for proteins with a role in cilia and flagella function were down-regulated in response to the stressor. In addition, while some of the transcripts related to biomineralisation were up-regulated (e.g. carbonic anhydrase 2, alkaline phosphatase), a small group was down-regulated (e.g. perlucin). Conclusions: This study highlighted the complex molecular response of the bivalve S. glomerata to expected near-future ocean acidification levels. While there are indications that the oyster attempted to adapt to the stressor, gauged by immune system priming and the increase in protein synthesis, some processes such cilia function appear to have been negatively affected by the elevated levels of CO2. Article in Journal/Newspaper Ocean acidification University of the Sunshine Coast, Queensland, Australia: COAST Research Database Climate Change Responses 3 1 |
institution |
Open Polar |
collection |
University of the Sunshine Coast, Queensland, Australia: COAST Research Database |
op_collection_id |
ftunivscoast |
language |
English |
topic |
FoR 0608 (Zoology) FoR 0601 (Biochemistry and Cell Biology) FoR 0604 (Genetics) saccostrea glomerata Sydney rock oyster molluscs RNA-seq stress carbon dioxide immunity biomineralisation |
spellingShingle |
FoR 0608 (Zoology) FoR 0601 (Biochemistry and Cell Biology) FoR 0604 (Genetics) saccostrea glomerata Sydney rock oyster molluscs RNA-seq stress carbon dioxide immunity biomineralisation Ertl, Nicole G O'Connor, W A Wiegand, A Elizur, A Molecular analysis of the Sydney rock oyster (Saccostrea glomerata) CO2 stress response |
topic_facet |
FoR 0608 (Zoology) FoR 0601 (Biochemistry and Cell Biology) FoR 0604 (Genetics) saccostrea glomerata Sydney rock oyster molluscs RNA-seq stress carbon dioxide immunity biomineralisation |
description |
Background: Human activities have led to a substantial increase in carbon dioxide (CO2) emission, with further increases predicted. A RNA-Seq study on adult Saccostrea glomerata was carried out to examine the molecular response of this bivalve species to elevated pCO2. Results: A total of 1626 S. glomerata transcripts were found to be differentially expressed in oysters exposed to elevated pCO2 when compared to control oysters. These transcripts cover a range of functions, from immunity (e.g. pattern recognition receptors, antimicrobial peptides), to respiration (e.g. antioxidants, mitochondrial respiratory chain proteins) and biomineralisation (e.g. carbonic anhydrase). Overall, elevated levels of CO2 appear to have resulted in a priming of the immune system and in producing countermeasures to potential oxidative stress. CO2 exposure also seems to have resulted in an increase in the expression of proteins involved in protein synthesis, whereas transcripts putatively coding for proteins with a role in cilia and flagella function were down-regulated in response to the stressor. In addition, while some of the transcripts related to biomineralisation were up-regulated (e.g. carbonic anhydrase 2, alkaline phosphatase), a small group was down-regulated (e.g. perlucin). Conclusions: This study highlighted the complex molecular response of the bivalve S. glomerata to expected near-future ocean acidification levels. While there are indications that the oyster attempted to adapt to the stressor, gauged by immune system priming and the increase in protein synthesis, some processes such cilia function appear to have been negatively affected by the elevated levels of CO2. |
format |
Article in Journal/Newspaper |
author |
Ertl, Nicole G O'Connor, W A Wiegand, A Elizur, A |
author_facet |
Ertl, Nicole G O'Connor, W A Wiegand, A Elizur, A |
author_sort |
Ertl, Nicole G |
title |
Molecular analysis of the Sydney rock oyster (Saccostrea glomerata) CO2 stress response |
title_short |
Molecular analysis of the Sydney rock oyster (Saccostrea glomerata) CO2 stress response |
title_full |
Molecular analysis of the Sydney rock oyster (Saccostrea glomerata) CO2 stress response |
title_fullStr |
Molecular analysis of the Sydney rock oyster (Saccostrea glomerata) CO2 stress response |
title_full_unstemmed |
Molecular analysis of the Sydney rock oyster (Saccostrea glomerata) CO2 stress response |
title_sort |
molecular analysis of the sydney rock oyster (saccostrea glomerata) co2 stress response |
publisher |
BioMed Central Ltd. |
publishDate |
2016 |
url |
https://doi.org/10.1186/s40665-016-0019-y |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
usc:20697 URN:ISSN: 2053-7565 |
op_rights |
Copyright © 2016 The Author(s). Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver(http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated |
op_rightsnorm |
CC0 PDM CC-BY |
op_doi |
https://doi.org/10.1186/s40665-016-0019-y |
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Climate Change Responses |
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3 |
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