Bacterial characterization of the snow cover at Spitzberg, Svalbard
International audience A sampling campaign was organized during spring 2004 in Spitzberg, Svalbard, in the area around the scientific base of Ny-Ålesund, to characterize the snow pack bacterial population. Total bacteria counts were established by 4',6-diamino-2-phenylindole (DAPI) in the seaso...
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ftunivsavoie:oai:HAL:insu-00375431v1 2024-05-12T07:59:40+00:00 Bacterial characterization of the snow cover at Spitzberg, Svalbard Amato, Pierre Hennebelle, Raphaëlle Magand, Olivier Sancelme, Martine Delort, A.M. Barbante, Carlo Boutron, Claude, F. Ferrari, Christophe, P. Synthèse et étude de systèmes à intêret biologique (SEESIB) Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de glaciologie et géophysique de l'environnement (LGGE) Observatoire des Sciences de l'Univers de Grenoble (OSUG) Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut national des sciences de l'Univers (INSU - CNRS)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut national des sciences de l'Univers (INSU - CNRS)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry )-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Environmental Sciences Department University of Ca’ Foscari Venice, Italy Polytech Grenoble 2006 https://insu.hal.science/insu-00375431 https://doi.org/10.1111/j.1574-6941.2006.00198.x en eng HAL CCSD Wiley-Blackwell info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-6941.2006.00198.x insu-00375431 https://insu.hal.science/insu-00375431 doi:10.1111/j.1574-6941.2006.00198.x ISSN: 0168-6496 EISSN: 1574-6941 FEMS Microbiology Ecology https://insu.hal.science/insu-00375431 FEMS Microbiology Ecology, 2006, 59 (2), pp.255 - 264. ⟨10.1111/j.1574-6941.2006.00198.x⟩ bacteria snow Arctic cold environment biodegradation [SDU.STU.GL]Sciences of the Universe [physics]/Earth Sciences/Glaciology info:eu-repo/semantics/article Journal articles 2006 ftunivsavoie https://doi.org/10.1111/j.1574-6941.2006.00198.x 2024-04-18T00:29:41Z International audience A sampling campaign was organized during spring 2004 in Spitzberg, Svalbard, in the area around the scientific base of Ny-Ålesund, to characterize the snow pack bacterial population. Total bacteria counts were established by 4',6-diamino-2-phenylindole (DAPI) in the seasonal snow pack bordering the sea. On the sea shore, bacterial concentration was about 6 × 104 cells mL−1, without any significant variation according to depth. In the accumulation snow layer of the glacier, concentrations were about 2 × 104 cells mL−1, except in the 2003 summer layer, where it reached 2 × 105 cells mL−1, as the result of cell multiplication allowed by higher temperature and snow melting. Strains isolated from the seasonal snow pack were identified from their 16S rRNA gene sequences, and lodged in GenBank. They belong to the Alphaproteobacteria, Betaproteobacteria and Gammaproteobacteria, Firmicutes and Actinobacteria. They are closely related to cold environment bacteria, as revealed by phylogenetic tree constructions, and two appear to be of unknown affiliation. Using 1H nuclear magnetic resonance, it was shown that these isolates have the capacity to degrade organic compounds found in Arctic snow (propionate, acetate and formate), and this can allow them to develop when snow melts, and thus to be actively involved in snow chemistry. Article in Journal/Newspaper Arctic glacier Ny Ålesund Ny-Ålesund Spitzberg Svalbard Université Savoie Mont Blanc: HAL Arctic Ny-Ålesund Svalbard FEMS Microbiology Ecology 59 2 255 264 |
institution |
Open Polar |
collection |
Université Savoie Mont Blanc: HAL |
op_collection_id |
ftunivsavoie |
language |
English |
topic |
bacteria snow Arctic cold environment biodegradation [SDU.STU.GL]Sciences of the Universe [physics]/Earth Sciences/Glaciology |
spellingShingle |
bacteria snow Arctic cold environment biodegradation [SDU.STU.GL]Sciences of the Universe [physics]/Earth Sciences/Glaciology Amato, Pierre Hennebelle, Raphaëlle Magand, Olivier Sancelme, Martine Delort, A.M. Barbante, Carlo Boutron, Claude, F. Ferrari, Christophe, P. Bacterial characterization of the snow cover at Spitzberg, Svalbard |
topic_facet |
bacteria snow Arctic cold environment biodegradation [SDU.STU.GL]Sciences of the Universe [physics]/Earth Sciences/Glaciology |
description |
International audience A sampling campaign was organized during spring 2004 in Spitzberg, Svalbard, in the area around the scientific base of Ny-Ålesund, to characterize the snow pack bacterial population. Total bacteria counts were established by 4',6-diamino-2-phenylindole (DAPI) in the seasonal snow pack bordering the sea. On the sea shore, bacterial concentration was about 6 × 104 cells mL−1, without any significant variation according to depth. In the accumulation snow layer of the glacier, concentrations were about 2 × 104 cells mL−1, except in the 2003 summer layer, where it reached 2 × 105 cells mL−1, as the result of cell multiplication allowed by higher temperature and snow melting. Strains isolated from the seasonal snow pack were identified from their 16S rRNA gene sequences, and lodged in GenBank. They belong to the Alphaproteobacteria, Betaproteobacteria and Gammaproteobacteria, Firmicutes and Actinobacteria. They are closely related to cold environment bacteria, as revealed by phylogenetic tree constructions, and two appear to be of unknown affiliation. Using 1H nuclear magnetic resonance, it was shown that these isolates have the capacity to degrade organic compounds found in Arctic snow (propionate, acetate and formate), and this can allow them to develop when snow melts, and thus to be actively involved in snow chemistry. |
author2 |
Synthèse et étude de systèmes à intêret biologique (SEESIB) Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de glaciologie et géophysique de l'environnement (LGGE) Observatoire des Sciences de l'Univers de Grenoble (OSUG) Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut national des sciences de l'Univers (INSU - CNRS)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut national des sciences de l'Univers (INSU - CNRS)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry )-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Environmental Sciences Department University of Ca’ Foscari Venice, Italy Polytech Grenoble |
format |
Article in Journal/Newspaper |
author |
Amato, Pierre Hennebelle, Raphaëlle Magand, Olivier Sancelme, Martine Delort, A.M. Barbante, Carlo Boutron, Claude, F. Ferrari, Christophe, P. |
author_facet |
Amato, Pierre Hennebelle, Raphaëlle Magand, Olivier Sancelme, Martine Delort, A.M. Barbante, Carlo Boutron, Claude, F. Ferrari, Christophe, P. |
author_sort |
Amato, Pierre |
title |
Bacterial characterization of the snow cover at Spitzberg, Svalbard |
title_short |
Bacterial characterization of the snow cover at Spitzberg, Svalbard |
title_full |
Bacterial characterization of the snow cover at Spitzberg, Svalbard |
title_fullStr |
Bacterial characterization of the snow cover at Spitzberg, Svalbard |
title_full_unstemmed |
Bacterial characterization of the snow cover at Spitzberg, Svalbard |
title_sort |
bacterial characterization of the snow cover at spitzberg, svalbard |
publisher |
HAL CCSD |
publishDate |
2006 |
url |
https://insu.hal.science/insu-00375431 https://doi.org/10.1111/j.1574-6941.2006.00198.x |
geographic |
Arctic Ny-Ålesund Svalbard |
geographic_facet |
Arctic Ny-Ålesund Svalbard |
genre |
Arctic glacier Ny Ålesund Ny-Ålesund Spitzberg Svalbard |
genre_facet |
Arctic glacier Ny Ålesund Ny-Ålesund Spitzberg Svalbard |
op_source |
ISSN: 0168-6496 EISSN: 1574-6941 FEMS Microbiology Ecology https://insu.hal.science/insu-00375431 FEMS Microbiology Ecology, 2006, 59 (2), pp.255 - 264. ⟨10.1111/j.1574-6941.2006.00198.x⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-6941.2006.00198.x insu-00375431 https://insu.hal.science/insu-00375431 doi:10.1111/j.1574-6941.2006.00198.x |
op_doi |
https://doi.org/10.1111/j.1574-6941.2006.00198.x |
container_title |
FEMS Microbiology Ecology |
container_volume |
59 |
container_issue |
2 |
container_start_page |
255 |
op_container_end_page |
264 |
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1798841251056844800 |