Sexing Free-Ranging Brown Bears Ursus-Arctos Using Hairs Found In The Field

• Published in: Molecular Ecology
• Main Authors: Taberlet, P., Mattock, H., Duboispaganon, C., Bouvet, J.
• Other Authors: Laboratoire d'Ecologie Alpine (LECA), Université Joseph Fourier - Grenoble 1 (UJF)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry )-Centre National de la Recherche Scientifique (CNRS)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 1993
• Subjects: HAIR; POLYMERASE CHAIN REACTION; SEX IDENTIFICATION; SRY GENE; URSUS-ARCTOS; Y-CHROMOSOME; [SDE.BE]Environmental Sciences/Biodiversity and Ecology; [SDV.BID]Life Sciences [q-bio]/Biodiversity; [SDV.EE]Life Sciences [q-bio]/Ecology; environment; Ursus arctos
• Online Access: https://hal.science/halsde-00281064; https://doi.org/10.1111/j.1365-294X.1993.tb00033.x

Times Cited: 58 International audience As an aid to the management of the Pyrenean population of the brown bear Ursus arctos, a sexing method based on the amplification of a Y chromosome specific sequence has been developed, and tested using hairs found in the field as a source of DNA. This method involves a two-step polymerase chain reaction (PCR) which allows the detection of a very small amount of DNA, probably a single SRY gene molecule. The sex can reliably be identified using about 50pg of DNA extract as template. It is possible that this approach could, with adjustments, be used to identify the sex of other species of eutherian mammals.