ASSESSING INTERACTIONS BETWEEN MERCURY AND MICROBIAL POPULATIONS IN THE SNOWPACK: A METAGENOMIC APPROACH
International audience Mercury (Hg), a persistent and toxic element, is found both naturally and as an anthropogenically-produced compound in the environment. Industrial use of Hg, and its subsequent release to the environment, has contributed to increasing Hg levels in soil, sediments and aquatic e...
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HAL CCSD
2009
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Online Access: | https://hal.science/hal-00399256 |
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ftunivsavoie:oai:HAL:hal-00399256v1 |
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openpolar |
institution |
Open Polar |
collection |
Université Savoie Mont Blanc: HAL |
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ftunivsavoie |
language |
English |
topic |
[SPI.NRJ]Engineering Sciences [physics]/Electric power [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
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[SPI.NRJ]Engineering Sciences [physics]/Electric power [SDE.BE]Environmental Sciences/Biodiversity and Ecology Larose, Catherine Berger, Sibel Ferrari, Christophe, P. Navarro, Elisabeth Dommergue, Aurélien Marusczak, Nicolas Cecillon, Sébastien Schneider, Dominique Vogel, Timothy M ASSESSING INTERACTIONS BETWEEN MERCURY AND MICROBIAL POPULATIONS IN THE SNOWPACK: A METAGENOMIC APPROACH |
topic_facet |
[SPI.NRJ]Engineering Sciences [physics]/Electric power [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
International audience Mercury (Hg), a persistent and toxic element, is found both naturally and as an anthropogenically-produced compound in the environment. Industrial use of Hg, and its subsequent release to the environment, has contributed to increasing Hg levels in soil, sediments and aquatic ecosystems worldwide. The Arctic is at risk for Hg toxicity. This is particularly the case for coastal sites, which appear to be involved in Hg cycling. The role of micro-organisms in the biogeochemical Hg cycle has recently become the focus of a large number of studies. The inorganic form of Hg, HgII, can be reduced to the relatively inert gaseous elemental mercury (GEM) form by bacteria possessing the mer operon. Bacteria, such as sulphate or iron reducers, are also reported to mediate the transformation of HgII to the more toxic and bioaccumulative organic form, methyl mercury (MeHg). However, the extent to which bacterial populations interact with mercury remains to be elucidated, especially in polar ecosystems. In order to understand how microbial populations respond to mercury, we used a metagenomic approach to describe both community structure and gene function variations in the Arctic snow pack over a two-month period during the spring of 2008 in Ny-Alesund, Svalbard, Norway. Using taxonomic DNA microarrays, a high throughput molecular biology technique, we monitored the evolution of bacterial communities within the snowpack. Community structure was then correlated to environmental factors and mercury concentrations. Gene function was analyzed by pyrosequencing as well as Q-PCR. The potential interdependence of the microbial community controlled mercury transformations and the community structure could provide insights into mercury cycling in the Arctic. In addition, the role of mercury resistant bacteria in the survival of the entire microbial community can be induced by the correlation between community members and mercury functional genes. |
author2 |
Ampère (AMPERE) École Centrale de Lyon (ECL) Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon) Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Laboratoire de glaciologie et géophysique de l'environnement (LGGE) Observatoire des Sciences de l'Univers de Grenoble (OSUG) Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut national des sciences de l'Univers (INSU - CNRS)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut national des sciences de l'Univers (INSU - CNRS)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry )-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Laboratoire Adaptation et pathogénie des micro-organismes Grenoble (LAPM) Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS) Laboratoire des symbioses tropicales et méditerranéennes (UMR LSTM) Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) |
format |
Conference Object |
author |
Larose, Catherine Berger, Sibel Ferrari, Christophe, P. Navarro, Elisabeth Dommergue, Aurélien Marusczak, Nicolas Cecillon, Sébastien Schneider, Dominique Vogel, Timothy M |
author_facet |
Larose, Catherine Berger, Sibel Ferrari, Christophe, P. Navarro, Elisabeth Dommergue, Aurélien Marusczak, Nicolas Cecillon, Sébastien Schneider, Dominique Vogel, Timothy M |
author_sort |
Larose, Catherine |
title |
ASSESSING INTERACTIONS BETWEEN MERCURY AND MICROBIAL POPULATIONS IN THE SNOWPACK: A METAGENOMIC APPROACH |
title_short |
ASSESSING INTERACTIONS BETWEEN MERCURY AND MICROBIAL POPULATIONS IN THE SNOWPACK: A METAGENOMIC APPROACH |
title_full |
ASSESSING INTERACTIONS BETWEEN MERCURY AND MICROBIAL POPULATIONS IN THE SNOWPACK: A METAGENOMIC APPROACH |
title_fullStr |
ASSESSING INTERACTIONS BETWEEN MERCURY AND MICROBIAL POPULATIONS IN THE SNOWPACK: A METAGENOMIC APPROACH |
title_full_unstemmed |
ASSESSING INTERACTIONS BETWEEN MERCURY AND MICROBIAL POPULATIONS IN THE SNOWPACK: A METAGENOMIC APPROACH |
title_sort |
assessing interactions between mercury and microbial populations in the snowpack: a metagenomic approach |
publisher |
HAL CCSD |
publishDate |
2009 |
url |
https://hal.science/hal-00399256 |
op_coverage |
Uppsala, Sweden |
genre |
Arctic Svalbard |
genre_facet |
Arctic Svalbard |
op_source |
BAGECO 10 : Bacterial Genetics and Ecology – Coexisting on a Changing Planet https://hal.science/hal-00399256 BAGECO 10 : Bacterial Genetics and Ecology – Coexisting on a Changing Planet, Jun 2009, Uppsala, Sweden http://hal.archives-ouvertes.fr/hal-00399256 |
op_relation |
hal-00399256 https://hal.science/hal-00399256 PRODINRA: 249186 |
_version_ |
1797577475081895936 |
spelling |
ftunivsavoie:oai:HAL:hal-00399256v1 2024-04-28T08:08:56+00:00 ASSESSING INTERACTIONS BETWEEN MERCURY AND MICROBIAL POPULATIONS IN THE SNOWPACK: A METAGENOMIC APPROACH Larose, Catherine Berger, Sibel Ferrari, Christophe, P. Navarro, Elisabeth Dommergue, Aurélien Marusczak, Nicolas Cecillon, Sébastien Schneider, Dominique Vogel, Timothy M Ampère (AMPERE) École Centrale de Lyon (ECL) Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon) Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Laboratoire de glaciologie et géophysique de l'environnement (LGGE) Observatoire des Sciences de l'Univers de Grenoble (OSUG) Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut national des sciences de l'Univers (INSU - CNRS)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut national des sciences de l'Univers (INSU - CNRS)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry )-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Laboratoire Adaptation et pathogénie des micro-organismes Grenoble (LAPM) Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS) Laboratoire des symbioses tropicales et méditerranéennes (UMR LSTM) Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) Uppsala, Sweden 2009-06-15 https://hal.science/hal-00399256 en eng HAL CCSD hal-00399256 https://hal.science/hal-00399256 PRODINRA: 249186 BAGECO 10 : Bacterial Genetics and Ecology – Coexisting on a Changing Planet https://hal.science/hal-00399256 BAGECO 10 : Bacterial Genetics and Ecology – Coexisting on a Changing Planet, Jun 2009, Uppsala, Sweden http://hal.archives-ouvertes.fr/hal-00399256 [SPI.NRJ]Engineering Sciences [physics]/Electric power [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/conferenceObject Conference papers 2009 ftunivsavoie 2024-04-11T00:30:17Z International audience Mercury (Hg), a persistent and toxic element, is found both naturally and as an anthropogenically-produced compound in the environment. Industrial use of Hg, and its subsequent release to the environment, has contributed to increasing Hg levels in soil, sediments and aquatic ecosystems worldwide. The Arctic is at risk for Hg toxicity. This is particularly the case for coastal sites, which appear to be involved in Hg cycling. The role of micro-organisms in the biogeochemical Hg cycle has recently become the focus of a large number of studies. The inorganic form of Hg, HgII, can be reduced to the relatively inert gaseous elemental mercury (GEM) form by bacteria possessing the mer operon. Bacteria, such as sulphate or iron reducers, are also reported to mediate the transformation of HgII to the more toxic and bioaccumulative organic form, methyl mercury (MeHg). However, the extent to which bacterial populations interact with mercury remains to be elucidated, especially in polar ecosystems. In order to understand how microbial populations respond to mercury, we used a metagenomic approach to describe both community structure and gene function variations in the Arctic snow pack over a two-month period during the spring of 2008 in Ny-Alesund, Svalbard, Norway. Using taxonomic DNA microarrays, a high throughput molecular biology technique, we monitored the evolution of bacterial communities within the snowpack. Community structure was then correlated to environmental factors and mercury concentrations. Gene function was analyzed by pyrosequencing as well as Q-PCR. The potential interdependence of the microbial community controlled mercury transformations and the community structure could provide insights into mercury cycling in the Arctic. In addition, the role of mercury resistant bacteria in the survival of the entire microbial community can be induced by the correlation between community members and mercury functional genes. Conference Object Arctic Svalbard Université Savoie Mont Blanc: HAL |