Diversity of bacteria producing pigmented colonies in aerosol, snow and soil samples from remote glacial areas (Antarctica, Alps and Andes)

• Main Authors: González-Toril, E., Amils, R., Delmas, Robert, Petit, J.-R., Komárek, J., Elster, J.
• Other Authors: Centro de Astrobiologia Madrid (CAB), Instituto Nacional de Técnica Aeroespacial (INTA)-Consejo Superior de Investigaciones Cientificas España = Spanish National Research Council Spain (CSIC), Centro de Biología Molecular (UAM-CSIC) Cantoblanco, Laboratoire de glaciologie et géophysique de l'environnement (LGGE), Observatoire des Sciences de l'Univers de Grenoble (OSUG), Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut national des sciences de l'Univers (INSU - CNRS)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut national des sciences de l'Univers (INSU - CNRS)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry )-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institute of Botany, Czech Academy of Sciences Prague (CAS)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2008
• Subjects: [PHYS.ASTR.CO]Physics [physics]/Astrophysics [astro-ph]/Cosmology and Extra-Galactic Astrophysics [astro-ph.CO]; [SDU.ASTR]Sciences of the Universe [physics]/Astrophysics [astro-ph]; [SDU.ENVI]Sciences of the Universe [physics]/Continental interfaces; environment; [SDU.OCEAN]Sciences of the Universe [physics]/Ocean; Atmosphere; [SDU.STU]Sciences of the Universe [physics]/Earth Sciences; Antarctic; The Antarctic; King George Island; Uruguay; Dumont d'Urville; Dumont-d'Urville; Artigas; Antarc*; Antarctica; Pseudonocardia antarctica
• Online Access: https://hal.science/hal-00330295; https://hal.science/hal-00330295/document; https://hal.science/hal-00330295/file/bgd-5-1607-2008.pdf

International audience Four different communities and one culture of pigmented microbial assemblages were obtained by incubation in mineral medium of samples collected from high elevation snow in the Alps (Mt. Blanc area) and the Andes (Nevado Illimani summit, Bolivia), from Antarctic aerosol (French station Dumont d'Urville) and a maritime Antarctic soil (King George Island, South Shetlands, Uruguay Station Artigas). Molecular analysis of more than 200 16S rRNA gene sequences showed that all cultured cells belong to the Bacteria domain. The phylogenetic comparison with the currently available rDNA database allowed the identification of sequences belonging to Proteobacteria ( Alpha-, Beta - and Gamma-proteobacteria ), Actinobacteria and Bacteroidetes phyla. The Andes snow culture was the richest in bacterial diversity (eight microorganisms identified) and the maritime Antarctic soil the poorest (only one). Snow samples from Col du midi (Alps) and the Andes shared the highest number of identified microorganisms ( Agrobacterium , Limnobacter , Aquiflexus and two uncultured Alphaproteobacteria clones). These two sampling sites also shared four sequences with the Antarctic aerosol sample ( Limnobacter , Pseudonocardia and an uncultured Alphaproteobacteria clone). The only microorganism identified in the maritime Antarctica soil ( Brevundimonas sp.) was also detected in the Antarctic aerosol. The two snow samples from the Alps only shared one common microorganism. Most of the identified microorganisms have been detected previously in cold environments ( Dietzia kujamenisi , Pseudonocardia Antarctica , Hydrogenophaga palleronii and Brebundimonas sp.), marine sediments ( Aquiflexus balticus , Pseudomonas pseudoalkaligenes , Pseudomonas sp. and one uncultured Alphaproteobacteria ), and soils and rocks ( Pseudonocardia sp., Agrobactrium sp., Limnobacter sp. and two uncultured Alphaproteobacetria clones). Air current dispersal is the best model to explain the presence of very specific microorganisms, like those used in ...