Notes on electropherograms of eye-lens, muscle proteins and zymograms of muscle esterases of fish collected during the first Brazilian expedition to the Antarctica

• Published in: Brazilian Journal of Oceanography
• Main Authors: Phan, Van Ngan, Suzuki, Hana, Gomes, Vicente, Passos, Maria José de Arruda Campos Rocha
• Format: Article in Journal/Newspaper
• Language: English
• Published: Universidade de São Paulo. Instituto Oceanográfico 1985
• Subjects: Peixes marinhos; Eletroforese; Esterases; Proteínas; Músculo; Cristalino; Notothenia larseni; Notothenia nudifrons; Electrona antarctica; N/Oc. "Prof. W. Besnard"; Estreito de Bransfield; Antártica; Marine fishes; Electrophoresis; Proteins; Muscle; Eye-lens; R/V "Prof. W. Besnard"; Bransfield Strait; Antarctica; Besnard; Antarc*
• Online Access: https://www.revistas.usp.br/biocean/article/view/6611

A preliminary study was carried out on electropherograms of eye-lens, muscle proteins and zymograms of muscle esterases of ten Notothenia larseni, six Notothenia nudifrons and one lanternfish, Electrona antarctica. The fish were collected by the R/V "Prof. W. Besnard" of the Institute of Oceanography, University of São Paulo, during the First Brazilian Expedition to Antarctica. Eye-lens proteins were analysed on cellulose acetate membrane, muscle proteins and esterases on gel of polyaorylamide. Eye-lens proteins showed three types of electropherograms for N. larseni, and two types for N. nudifrons. One of the electropherograms of N. larseni can be readily distinguished from those of N. nudifrons. Electropherograms of muscle proteins of N. larseni and N. nudifrons are very similar and, consist of sixteen to seventeen fractions. Electropherograms of muscle proteins of N. larseni are severely affected by the conservation of the extracts overnight under -20ºC. All N. nudifrons were of the same zymograms of esterases while those of N. larseni varied. Electropherograms of eye-lens and muscle proteins as well as zymograms of esterases of the lanternfish are different from those of nototheniids. Foi realizado um estudo preliminar sobre eletroferogramas de proteínas de cristalino e de músculo esquelético, e zimogramas de esterases de músculo esquelético de dez Notothenia larseni, seis Notothenia nudifrons e de um peixe-lanterna, Electrona antarctica. Os peixes foram coletados pelo N/Oc. "Prof. W. Besnard" do Instituto Oceanográfico da Universidade de São Paulo durante a I Expedição Brasileira à Antártica. As proteinas do cristalino foram analisadas em membranas de acetato de celulose, enquanto que as proteínas e esterases do músculo esquelético, em gel de poliacrilamida. As proteínas do cristalino apresentam três tipos distintos de eletroferogramas para N. larseni, e dois para N. nudifrons. Um dos eletroferogramas de N. larseni, pode ser prontamente distinguido dos de N. nudifrons. Eletrof erogramas de proteínas de ...