Wild birds of Chile as bioindicators of carbapenemase- or extended spectrum β -lactamase-producing Enterobacterales
Antimicrobial-resistant bacteria are a major threat to human and animal health. Among these organisms, extended-spectrum beta-lactamase (ESBL)- or carbapenemase- producing Enterobacterales commonly cause infections both in human and veterinary medicine. Actually, are considered global critical-prior...
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Format: | Doctoral or Postdoctoral Thesis |
Language: | English |
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Biblioteca Digital de Teses e Dissertações da USP
2021
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Online Access: | https://www.teses.usp.br/teses/disponiveis/10/10133/tde-26082021-113658/ https://doi.org/10.11606/T.10.2021.tde-26082021-113658 |
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ftunivsaopaulo:oai:teses.usp.br:tde-26082021-113658 2023-12-24T10:09:01+01:00 Wild birds of Chile as bioindicators of carbapenemase- or extended spectrum β -lactamase-producing Enterobacterales Aves selvagens do Chile como indicadores biológicos de Enterobacterales portadores de carbapenemases ou beta-lactamases de espectro estendido Castillo, Danny Andrés Fuentes Dias, José Luiz Catão 2021-06-24 application/pdf https://www.teses.usp.br/teses/disponiveis/10/10133/tde-26082021-113658/ https://doi.org/10.11606/T.10.2021.tde-26082021-113658 en eng Biblioteca Digital de Teses e Dissertações da USP Universidade de São Paulo Faculdade de Medicina Veterinária e Zootecnia https://www.teses.usp.br/teses/disponiveis/10/10133/tde-26082021-113658/ doi:10.11606/T.10.2021.tde-26082021-113658 Liberar o conteúdo para acesso público. Fauna silvestre BLEE Resistência antimicrobiana CTX-M NDM-5 Antimicrobial resistance ESBL Wildlife Tese (Doutorado) 2021 ftunivsaopaulo https://doi.org/10.11606/T.10.2021.tde-26082021-113658 2023-11-26T00:16:13Z Antimicrobial-resistant bacteria are a major threat to human and animal health. Among these organisms, extended-spectrum beta-lactamase (ESBL)- or carbapenemase- producing Enterobacterales commonly cause infections both in human and veterinary medicine. Actually, are considered global critical-priority pathogens by the World Health Organization (WHO) with a urgence in research and development of effective drugs and treatments. Recent studies reports that wild animals have been colonized by ESBL- or carbapenemase-producing Enterobacterales, nevertheless, critical gaps on the knowledge about the real role of wildlife in the evolution and spread of these “superbugs”. In this epidemiological surveillance study, using microbiologic and whole-genome sequencing tools, we analyzed wild bird samples admitted at wildlife rehabilitation centers (WRC) and free- living birds from several eco-regions of Chile, including the Andes Highlands, Atacama Desert, Chiloé Archipelago and Patagonia, besides Antarctica. Our results reveal that wild owls and Andean condors were colonized by international clones of ESBL CTX- M-types-carrying Escherichia coli. An owl (Bubo magellanicus) was also colonized by a Salmonella enterica ser. Infantis belonged to the sequence type (ST) 32 carrying a blaCTX-65M gene. On other hand, in free-living birds, was registered a migratory bird (Numenius phaeopus) colonized by an Serratia fonticola bearing the ESBL chromosomal gene FONA-type; whole-genome sequencing and “ in silico” studies determined that belonged to a novel variant, designed as FONA-7. In samples of five wild Andean condors that fed on landfills at the Andes Highlands, we founded E. coli producers of carbapenemase NDM-5 and BLEE (SHV-12 e CTX-M-177) genes. Finally, in a Chiloé Archipelago’s Island, South Chile, CTX-M-1 and CTX-M-55- producing E. coli were isolated from three migratory gulls (Leucophaeus pipixcan) and two resident gulls (Chroicocephalus maculipennis). ESBL- or carbapenemase- producing Enterobacterales cause nosocomial ... Doctoral or Postdoctoral Thesis Antarc* Antarctica Numenius phaeopus University of São Paulo: Digital Library of Theses and Dissertations Chiloé ENVELOPE(-63.983,-63.983,-65.517,-65.517) Patagonia |
institution |
Open Polar |
collection |
University of São Paulo: Digital Library of Theses and Dissertations |
op_collection_id |
ftunivsaopaulo |
language |
English |
topic |
Fauna silvestre BLEE Resistência antimicrobiana CTX-M NDM-5 Antimicrobial resistance ESBL Wildlife |
spellingShingle |
Fauna silvestre BLEE Resistência antimicrobiana CTX-M NDM-5 Antimicrobial resistance ESBL Wildlife Castillo, Danny Andrés Fuentes Wild birds of Chile as bioindicators of carbapenemase- or extended spectrum β -lactamase-producing Enterobacterales |
topic_facet |
Fauna silvestre BLEE Resistência antimicrobiana CTX-M NDM-5 Antimicrobial resistance ESBL Wildlife |
description |
Antimicrobial-resistant bacteria are a major threat to human and animal health. Among these organisms, extended-spectrum beta-lactamase (ESBL)- or carbapenemase- producing Enterobacterales commonly cause infections both in human and veterinary medicine. Actually, are considered global critical-priority pathogens by the World Health Organization (WHO) with a urgence in research and development of effective drugs and treatments. Recent studies reports that wild animals have been colonized by ESBL- or carbapenemase-producing Enterobacterales, nevertheless, critical gaps on the knowledge about the real role of wildlife in the evolution and spread of these “superbugs”. In this epidemiological surveillance study, using microbiologic and whole-genome sequencing tools, we analyzed wild bird samples admitted at wildlife rehabilitation centers (WRC) and free- living birds from several eco-regions of Chile, including the Andes Highlands, Atacama Desert, Chiloé Archipelago and Patagonia, besides Antarctica. Our results reveal that wild owls and Andean condors were colonized by international clones of ESBL CTX- M-types-carrying Escherichia coli. An owl (Bubo magellanicus) was also colonized by a Salmonella enterica ser. Infantis belonged to the sequence type (ST) 32 carrying a blaCTX-65M gene. On other hand, in free-living birds, was registered a migratory bird (Numenius phaeopus) colonized by an Serratia fonticola bearing the ESBL chromosomal gene FONA-type; whole-genome sequencing and “ in silico” studies determined that belonged to a novel variant, designed as FONA-7. In samples of five wild Andean condors that fed on landfills at the Andes Highlands, we founded E. coli producers of carbapenemase NDM-5 and BLEE (SHV-12 e CTX-M-177) genes. Finally, in a Chiloé Archipelago’s Island, South Chile, CTX-M-1 and CTX-M-55- producing E. coli were isolated from three migratory gulls (Leucophaeus pipixcan) and two resident gulls (Chroicocephalus maculipennis). ESBL- or carbapenemase- producing Enterobacterales cause nosocomial ... |
author2 |
Dias, José Luiz Catão |
format |
Doctoral or Postdoctoral Thesis |
author |
Castillo, Danny Andrés Fuentes |
author_facet |
Castillo, Danny Andrés Fuentes |
author_sort |
Castillo, Danny Andrés Fuentes |
title |
Wild birds of Chile as bioindicators of carbapenemase- or extended spectrum β -lactamase-producing Enterobacterales |
title_short |
Wild birds of Chile as bioindicators of carbapenemase- or extended spectrum β -lactamase-producing Enterobacterales |
title_full |
Wild birds of Chile as bioindicators of carbapenemase- or extended spectrum β -lactamase-producing Enterobacterales |
title_fullStr |
Wild birds of Chile as bioindicators of carbapenemase- or extended spectrum β -lactamase-producing Enterobacterales |
title_full_unstemmed |
Wild birds of Chile as bioindicators of carbapenemase- or extended spectrum β -lactamase-producing Enterobacterales |
title_sort |
wild birds of chile as bioindicators of carbapenemase- or extended spectrum β -lactamase-producing enterobacterales |
publisher |
Biblioteca Digital de Teses e Dissertações da USP |
publishDate |
2021 |
url |
https://www.teses.usp.br/teses/disponiveis/10/10133/tde-26082021-113658/ https://doi.org/10.11606/T.10.2021.tde-26082021-113658 |
long_lat |
ENVELOPE(-63.983,-63.983,-65.517,-65.517) |
geographic |
Chiloé Patagonia |
geographic_facet |
Chiloé Patagonia |
genre |
Antarc* Antarctica Numenius phaeopus |
genre_facet |
Antarc* Antarctica Numenius phaeopus |
op_relation |
https://www.teses.usp.br/teses/disponiveis/10/10133/tde-26082021-113658/ doi:10.11606/T.10.2021.tde-26082021-113658 |
op_rights |
Liberar o conteúdo para acesso público. |
op_doi |
https://doi.org/10.11606/T.10.2021.tde-26082021-113658 |
_version_ |
1786205358540193792 |