Phaeurus antarcticus and its endophytic fungi: chemical diversity of a hidden pharmacy underneath the Antarctic Ocean
Chapter I: The chemical profile and antibacterial, antiparasitic, and insecticidal activities of the seaweed P. antarcticus were investigated. The hexane extract (HX) and its fractions exhibited selective antibacterial activity against Staphylococcus aureus. Fractions HX-FC and HX-FD showed the high...
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Biblioteca Digital de Teses e Dissertações da USP
2022
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Online Access: | https://www.teses.usp.br/teses/disponiveis/60/60138/tde-20072023-113231/ https://doi.org/10.11606/T.60.2022.tde-20072023-113231 |
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ftunivsaopaulo:oai:teses.usp.br:tde-20072023-113231 2023-09-26T15:10:16+02:00 Phaeurus antarcticus and its endophytic fungi: chemical diversity of a hidden pharmacy underneath the Antarctic Ocean Phaeurus antarcticus e seus fungos endofíticos: diversidade química de uma farmácia oculta sob o oceano Antártico Santos, Gustavo Souza dos Debonsi, Hosana Maria Edrada-ebel, Ru Angelie 2022-11-29 application/pdf https://www.teses.usp.br/teses/disponiveis/60/60138/tde-20072023-113231/ https://doi.org/10.11606/T.60.2022.tde-20072023-113231 en eng Biblioteca Digital de Teses e Dissertações da USP Universidade de São Paulo Faculdade de Ciências Farmacêuticas de Ribeirão Preto https://www.teses.usp.br/teses/disponiveis/60/60138/tde-20072023-113231/ doi:10.11606/T.60.2022.tde-20072023-113231 Reter o conteúdo por motivos de patente, publicação e/ou direitos autoriais. Produtos naturais marinhos Macroalgas antárticas Fungos antárticos Fotoproteção Doenças negligenciadas Atividade imunomoduladora Antimicrobianos Metabolômica Neglected diseases Metabolomics Photoprotection Marine natural products Immunomodulatory activity Antimicrobials Antarctic seaweed Antarctic fungi Tese (Doutorado) 2022 ftunivsaopaulo https://doi.org/10.11606/T.60.2022.tde-20072023-113231 2023-08-26T23:15:19Z Chapter I: The chemical profile and antibacterial, antiparasitic, and insecticidal activities of the seaweed P. antarcticus were investigated. The hexane extract (HX) and its fractions exhibited selective antibacterial activity against Staphylococcus aureus. Fractions HX-FC and HX-FD showed the highest antiparasitic activity against Leishmania amazonensis and Neospora caninum. The HX extract and the HX-FC fraction presented insecticidal potential against Aedes aegypti larvae. Through GC-MS analysis and the combination of multivariate statistical analyses (OPLS-DA) and the construction of molecular networks (GNPS), it was possible to annotate terpenes, steroids, fatty acids, and alcohols in the bioactive fractions. Chapter II: Using metabolomics techniques (UPLC-HRMS/1H NMR) and multivariate statistical analysis (PCA, OPLS-DA), the spatial variability of secondary metabolites of the seaweed P. antarcticus and the antibiofilm activity of extracts and fractions were evaluated against S. aureus resistant to methicillin-oxacillin (MRSA). The results revealed that the changes in the chemical profile were not site-specific and could be related to different life cycle stages of the collected algae. Porphyrins, terpenes, and carotenoids were annotated as discriminating compounds. Linoleic acid (1) and fucosterol (2) were isolated from the bioactive fractions and showed antibiofilm activity against MRSA. Chapter III: The extract and fractions of the fungus Penicillium purpurogenum were evaluated for antibacterial, antiparasitic, immunomodulatory, and photoprotective activities. The bioguided fractionation led to the isolation of six polyketides: 5,6,8-trihydroxy-4-(1\'-hydroxyethyl) isocoumarin (3), aspergillumarin A (4), aspergillumarin B (5), Berkeleyacetal C (6), sescandelin (7), and vermistatin (8). Compounds 3, 4, 7, and 8 presented photostability and photoprotective activities against UVB-UVA. Compounds 3, 6, 7, and 8 presented immunomodulatory activity by down-regulating the production of reactive oxygen species in ... Doctoral or Postdoctoral Thesis Antarc* Antarctic Antarctic Ocean antarcticus University of São Paulo: Digital Library of Theses and Dissertations Antarctic The Antarctic Antarctic Ocean Sob’ ENVELOPE(66.156,66.156,66.322,66.322) |
institution |
Open Polar |
collection |
University of São Paulo: Digital Library of Theses and Dissertations |
op_collection_id |
ftunivsaopaulo |
language |
English |
topic |
Produtos naturais marinhos Macroalgas antárticas Fungos antárticos Fotoproteção Doenças negligenciadas Atividade imunomoduladora Antimicrobianos Metabolômica Neglected diseases Metabolomics Photoprotection Marine natural products Immunomodulatory activity Antimicrobials Antarctic seaweed Antarctic fungi |
spellingShingle |
Produtos naturais marinhos Macroalgas antárticas Fungos antárticos Fotoproteção Doenças negligenciadas Atividade imunomoduladora Antimicrobianos Metabolômica Neglected diseases Metabolomics Photoprotection Marine natural products Immunomodulatory activity Antimicrobials Antarctic seaweed Antarctic fungi Santos, Gustavo Souza dos Phaeurus antarcticus and its endophytic fungi: chemical diversity of a hidden pharmacy underneath the Antarctic Ocean |
topic_facet |
Produtos naturais marinhos Macroalgas antárticas Fungos antárticos Fotoproteção Doenças negligenciadas Atividade imunomoduladora Antimicrobianos Metabolômica Neglected diseases Metabolomics Photoprotection Marine natural products Immunomodulatory activity Antimicrobials Antarctic seaweed Antarctic fungi |
description |
Chapter I: The chemical profile and antibacterial, antiparasitic, and insecticidal activities of the seaweed P. antarcticus were investigated. The hexane extract (HX) and its fractions exhibited selective antibacterial activity against Staphylococcus aureus. Fractions HX-FC and HX-FD showed the highest antiparasitic activity against Leishmania amazonensis and Neospora caninum. The HX extract and the HX-FC fraction presented insecticidal potential against Aedes aegypti larvae. Through GC-MS analysis and the combination of multivariate statistical analyses (OPLS-DA) and the construction of molecular networks (GNPS), it was possible to annotate terpenes, steroids, fatty acids, and alcohols in the bioactive fractions. Chapter II: Using metabolomics techniques (UPLC-HRMS/1H NMR) and multivariate statistical analysis (PCA, OPLS-DA), the spatial variability of secondary metabolites of the seaweed P. antarcticus and the antibiofilm activity of extracts and fractions were evaluated against S. aureus resistant to methicillin-oxacillin (MRSA). The results revealed that the changes in the chemical profile were not site-specific and could be related to different life cycle stages of the collected algae. Porphyrins, terpenes, and carotenoids were annotated as discriminating compounds. Linoleic acid (1) and fucosterol (2) were isolated from the bioactive fractions and showed antibiofilm activity against MRSA. Chapter III: The extract and fractions of the fungus Penicillium purpurogenum were evaluated for antibacterial, antiparasitic, immunomodulatory, and photoprotective activities. The bioguided fractionation led to the isolation of six polyketides: 5,6,8-trihydroxy-4-(1\'-hydroxyethyl) isocoumarin (3), aspergillumarin A (4), aspergillumarin B (5), Berkeleyacetal C (6), sescandelin (7), and vermistatin (8). Compounds 3, 4, 7, and 8 presented photostability and photoprotective activities against UVB-UVA. Compounds 3, 6, 7, and 8 presented immunomodulatory activity by down-regulating the production of reactive oxygen species in ... |
author2 |
Debonsi, Hosana Maria Edrada-ebel, Ru Angelie |
format |
Doctoral or Postdoctoral Thesis |
author |
Santos, Gustavo Souza dos |
author_facet |
Santos, Gustavo Souza dos |
author_sort |
Santos, Gustavo Souza dos |
title |
Phaeurus antarcticus and its endophytic fungi: chemical diversity of a hidden pharmacy underneath the Antarctic Ocean |
title_short |
Phaeurus antarcticus and its endophytic fungi: chemical diversity of a hidden pharmacy underneath the Antarctic Ocean |
title_full |
Phaeurus antarcticus and its endophytic fungi: chemical diversity of a hidden pharmacy underneath the Antarctic Ocean |
title_fullStr |
Phaeurus antarcticus and its endophytic fungi: chemical diversity of a hidden pharmacy underneath the Antarctic Ocean |
title_full_unstemmed |
Phaeurus antarcticus and its endophytic fungi: chemical diversity of a hidden pharmacy underneath the Antarctic Ocean |
title_sort |
phaeurus antarcticus and its endophytic fungi: chemical diversity of a hidden pharmacy underneath the antarctic ocean |
publisher |
Biblioteca Digital de Teses e Dissertações da USP |
publishDate |
2022 |
url |
https://www.teses.usp.br/teses/disponiveis/60/60138/tde-20072023-113231/ https://doi.org/10.11606/T.60.2022.tde-20072023-113231 |
long_lat |
ENVELOPE(66.156,66.156,66.322,66.322) |
geographic |
Antarctic The Antarctic Antarctic Ocean Sob’ |
geographic_facet |
Antarctic The Antarctic Antarctic Ocean Sob’ |
genre |
Antarc* Antarctic Antarctic Ocean antarcticus |
genre_facet |
Antarc* Antarctic Antarctic Ocean antarcticus |
op_relation |
https://www.teses.usp.br/teses/disponiveis/60/60138/tde-20072023-113231/ doi:10.11606/T.60.2022.tde-20072023-113231 |
op_rights |
Reter o conteúdo por motivos de patente, publicação e/ou direitos autoriais. |
op_doi |
https://doi.org/10.11606/T.60.2022.tde-20072023-113231 |
_version_ |
1778142318774190080 |