Consolidation of the genetic and cytogenetic maps of turbot (Scophthalmus maximus) using FISH with BAC clones
This is a post-peer-review, pre-copyedit version of an article published in Chromosoma. The final authenticated version is available online at: https://doi.org/10.1007/s00412-014-0452-2 Bacterial artificial chromosomes (BAC) have been widely used for fluorescence in situ hybridization (FISH) mapping...
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ftunivsantcomp:oai:minerva.usc.es:10347/26301 2023-07-30T04:06:40+02:00 Consolidation of the genetic and cytogenetic maps of turbot (Scophthalmus maximus) using FISH with BAC clones Taboada Penoucos, Xoana Pansonato Alves, José Carlos Foresti, Fausto Martínez Portela, Paulino Viñas Díaz, Ana María Gómez Pardo, María Belén Bouza Fernández, María Carmen Universidade de Santiago de Compostela. Departamento de Xenética application/pdf http://hdl.handle.net/10347/26301 https://doi.org/10.1007/s00412-014-0452-2 eng eng Springer https://doi.org/10.1007/s00412-014-0452-2 info:eu-repo/grantAgreement/MICINN/ Plan Nacional de I+D+i 2008-2011/AGL2009-13273/ES/Extension Del Analisis Genomico Estructural En El Rodaballo: Mapeo Comparativo E Integracion Del Mapeo Genetico, Cromosomico Y Fisico %7Cinfo:eu-repo/grantAgreement/MEC/Plan Nacional de I+D+i 2004-2007/CSD2007-00002/ES/Mejora de la producción en acuicultura mediante herramientas de biotecnología (Aquagenomics) Taboada, X., Pansonato-Alves, J.C., Foresti, F. et al. Consolidation of the genetic and cytogenetic maps of turbot (Scophthalmus maximus) using FISH with BAC clones. Chromosoma 123, 281–291 (2014). https://doi.org/10.1007/s00412-014-0452-2 0009-5915 http://hdl.handle.net/10347/26301 doi:10.1007/s00412-014-0452-2 1432-0886 © Springer-Verlag Berlin Heidelberg 2014. This article may be used for non-commercial purposes in accordance with Springer Verlag Terms and Conditions for self-archiving info:eu-repo/semantics/openAccess BAC library BAC-FISH Genetic map Cytogenetic map Turbot info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion ftunivsantcomp https://doi.org/10.1007/s00412-014-0452-2 2023-07-11T23:26:15Z This is a post-peer-review, pre-copyedit version of an article published in Chromosoma. The final authenticated version is available online at: https://doi.org/10.1007/s00412-014-0452-2 Bacterial artificial chromosomes (BAC) have been widely used for fluorescence in situ hybridization (FISH) mapping of chromosome landmarks in different organisms, including a few in teleosts. In this study, we used BAC-FISH to consolidate the previous genetic and cytogenetic maps of the turbot (Scophthalmus maximus), a commercially important pleuronectiform. The maps consisted of 24 linkage groups (LGs) but only 22 chromosomes. All turbot LGs were assigned to specific chromosomes using BAC probes obtained from a turbot 5× genomic BAC library. It consisted of 46,080 clones with inserts of at least 100 kb and <5 % empty vectors. These BAC probes contained gene-derived or anonymous markers, most of them linked to quantitative trait loci (QTL) related to productive traits. BAC clones were mapped by FISH to unique marker-specific chromosomal positions, which showed a notable concordance with previous genetic mapping data. The two metacentric pairs were cytogenetically assigned to LG2 and LG16, and the nucleolar organizer region (NOR)-bearing pair was assigned to LG15. Double-color FISH assays enabled the consolidation of the turbot genetic map into 22 linkage groups by merging LG8 with LG18 and LG21 with LG24. In this work, a first-generation probe panel of BAC clones anchored to the turbot linkage and cytogenetical map was developed. It is a useful tool for chromosome traceability in turbot, but also relevant in the context of pleuronectiform karyotypes, which often show small hardly identifiable chromosomes. This panel will also be valuable for further integrative genomics of turbot within Pleuronectiformes and teleosts, especially for fine QTL mapping for aquaculture traits, comparative genomics, and whole-genome assembly This study was supported by Spain’s Ministerio de Ciencia e Innovación (AGL2009-13273), Consolider ... Article in Journal/Newspaper Scophthalmus maximus Turbot Minerva - Repositorio institucional da Universidade de Santiago de Compostela (USC) Chromosoma 123 3 281 291 |
institution |
Open Polar |
collection |
Minerva - Repositorio institucional da Universidade de Santiago de Compostela (USC) |
op_collection_id |
ftunivsantcomp |
language |
English |
topic |
BAC library BAC-FISH Genetic map Cytogenetic map Turbot |
spellingShingle |
BAC library BAC-FISH Genetic map Cytogenetic map Turbot Taboada Penoucos, Xoana Pansonato Alves, José Carlos Foresti, Fausto Martínez Portela, Paulino Viñas Díaz, Ana María Gómez Pardo, María Belén Bouza Fernández, María Carmen Consolidation of the genetic and cytogenetic maps of turbot (Scophthalmus maximus) using FISH with BAC clones |
topic_facet |
BAC library BAC-FISH Genetic map Cytogenetic map Turbot |
description |
This is a post-peer-review, pre-copyedit version of an article published in Chromosoma. The final authenticated version is available online at: https://doi.org/10.1007/s00412-014-0452-2 Bacterial artificial chromosomes (BAC) have been widely used for fluorescence in situ hybridization (FISH) mapping of chromosome landmarks in different organisms, including a few in teleosts. In this study, we used BAC-FISH to consolidate the previous genetic and cytogenetic maps of the turbot (Scophthalmus maximus), a commercially important pleuronectiform. The maps consisted of 24 linkage groups (LGs) but only 22 chromosomes. All turbot LGs were assigned to specific chromosomes using BAC probes obtained from a turbot 5× genomic BAC library. It consisted of 46,080 clones with inserts of at least 100 kb and <5 % empty vectors. These BAC probes contained gene-derived or anonymous markers, most of them linked to quantitative trait loci (QTL) related to productive traits. BAC clones were mapped by FISH to unique marker-specific chromosomal positions, which showed a notable concordance with previous genetic mapping data. The two metacentric pairs were cytogenetically assigned to LG2 and LG16, and the nucleolar organizer region (NOR)-bearing pair was assigned to LG15. Double-color FISH assays enabled the consolidation of the turbot genetic map into 22 linkage groups by merging LG8 with LG18 and LG21 with LG24. In this work, a first-generation probe panel of BAC clones anchored to the turbot linkage and cytogenetical map was developed. It is a useful tool for chromosome traceability in turbot, but also relevant in the context of pleuronectiform karyotypes, which often show small hardly identifiable chromosomes. This panel will also be valuable for further integrative genomics of turbot within Pleuronectiformes and teleosts, especially for fine QTL mapping for aquaculture traits, comparative genomics, and whole-genome assembly This study was supported by Spain’s Ministerio de Ciencia e Innovación (AGL2009-13273), Consolider ... |
author2 |
Universidade de Santiago de Compostela. Departamento de Xenética |
format |
Article in Journal/Newspaper |
author |
Taboada Penoucos, Xoana Pansonato Alves, José Carlos Foresti, Fausto Martínez Portela, Paulino Viñas Díaz, Ana María Gómez Pardo, María Belén Bouza Fernández, María Carmen |
author_facet |
Taboada Penoucos, Xoana Pansonato Alves, José Carlos Foresti, Fausto Martínez Portela, Paulino Viñas Díaz, Ana María Gómez Pardo, María Belén Bouza Fernández, María Carmen |
author_sort |
Taboada Penoucos, Xoana |
title |
Consolidation of the genetic and cytogenetic maps of turbot (Scophthalmus maximus) using FISH with BAC clones |
title_short |
Consolidation of the genetic and cytogenetic maps of turbot (Scophthalmus maximus) using FISH with BAC clones |
title_full |
Consolidation of the genetic and cytogenetic maps of turbot (Scophthalmus maximus) using FISH with BAC clones |
title_fullStr |
Consolidation of the genetic and cytogenetic maps of turbot (Scophthalmus maximus) using FISH with BAC clones |
title_full_unstemmed |
Consolidation of the genetic and cytogenetic maps of turbot (Scophthalmus maximus) using FISH with BAC clones |
title_sort |
consolidation of the genetic and cytogenetic maps of turbot (scophthalmus maximus) using fish with bac clones |
publisher |
Springer |
url |
http://hdl.handle.net/10347/26301 https://doi.org/10.1007/s00412-014-0452-2 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
https://doi.org/10.1007/s00412-014-0452-2 info:eu-repo/grantAgreement/MICINN/ Plan Nacional de I+D+i 2008-2011/AGL2009-13273/ES/Extension Del Analisis Genomico Estructural En El Rodaballo: Mapeo Comparativo E Integracion Del Mapeo Genetico, Cromosomico Y Fisico %7Cinfo:eu-repo/grantAgreement/MEC/Plan Nacional de I+D+i 2004-2007/CSD2007-00002/ES/Mejora de la producción en acuicultura mediante herramientas de biotecnología (Aquagenomics) Taboada, X., Pansonato-Alves, J.C., Foresti, F. et al. Consolidation of the genetic and cytogenetic maps of turbot (Scophthalmus maximus) using FISH with BAC clones. Chromosoma 123, 281–291 (2014). https://doi.org/10.1007/s00412-014-0452-2 0009-5915 http://hdl.handle.net/10347/26301 doi:10.1007/s00412-014-0452-2 1432-0886 |
op_rights |
© Springer-Verlag Berlin Heidelberg 2014. This article may be used for non-commercial purposes in accordance with Springer Verlag Terms and Conditions for self-archiving info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1007/s00412-014-0452-2 |
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