The hemoglobin Gly16β1Asp polymorphism in turbot (Scophthalmus maximus) is differentially distributed across European populations
Turbot is an important flatfish widely distributed along the European coasts, whose fishery is centered in the North Sea. The commercial value of the species has boosted a successful aquaculture sector in Europe and China. Body growth is the main target of turbot breeding programs and is also a key...
Published in: | Fish Physiology and Biochemistry |
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Main Authors: | , , , |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Springer
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Online Access: | http://hdl.handle.net/10347/24213 https://doi.org/10.1007/s10695-020-00872-y |
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ftunivsantcomp:oai:minerva.usc.es:10347/24213 |
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Open Polar |
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Minerva - Repositorio institucional da Universidade de Santiago de Compostela (USC) |
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ftunivsantcomp |
language |
English |
topic |
Turbot Hemoglobin Polymorphism Body growth Genetic variation Adaptation |
spellingShingle |
Turbot Hemoglobin Polymorphism Body growth Genetic variation Adaptation Andersen, Øivind Rubiolo Gaytán, Juan Andrés Rosa, Maria Cristina de Martínez Portela, Paulino The hemoglobin Gly16β1Asp polymorphism in turbot (Scophthalmus maximus) is differentially distributed across European populations |
topic_facet |
Turbot Hemoglobin Polymorphism Body growth Genetic variation Adaptation |
description |
Turbot is an important flatfish widely distributed along the European coasts, whose fishery is centered in the North Sea. The commercial value of the species has boosted a successful aquaculture sector in Europe and China. Body growth is the main target of turbot breeding programs and is also a key trait related to local adaptation to temperature and salinity. Differences in growth rate and optimal growth temperature in turbot have been shown to be associated with a hemoglobin polymorphism reported more than 50 years ago. Here, we identified a Gly16Asp amino acid substitution in the β1 globin subunit by searching for genetic variation in the five functional globin genes within the whole annotated turbot genome. We predicted increased stability of the turbot hemoglobin by the replacement of the conserved Gly with the negative charged Asp residue that is consistent with the higher rate of αβ dimer assembly in the human J-Baltimore Gly16β->Asp mutant than in normal HbA. The turbot Hbβ1-Gly16 variant dominated in the northern populations examined, particularly in the Baltic Sea, while the Asp allele showed elevated frequencies in southern populations and was the prevalent variant in the Adriatic Sea. Body weight did not associate with the Hbβ1 genotypes at farming conditions (i.e., high oxygen levels, feeding ad libitum) after analyzing 90 fish with high growth dispersal from nine turbot families. Nevertheless, all data at hand suggest that the turbot hemoglobin polymorphism has an adaptive significance in the variable wild conditions regarding temperature and oxygen availability Open Access funding provided by Norwegian University of Life Sciences. This work was supported by the EU projects AQUATRACE (no. 311920) and FISHBOOST (no. 613611) SI |
author2 |
Universidade de Santiago de Compostela. Departamento de Zooloxía, Xenética e Antropoloxía Física |
format |
Article in Journal/Newspaper |
author |
Andersen, Øivind Rubiolo Gaytán, Juan Andrés Rosa, Maria Cristina de Martínez Portela, Paulino |
author_facet |
Andersen, Øivind Rubiolo Gaytán, Juan Andrés Rosa, Maria Cristina de Martínez Portela, Paulino |
author_sort |
Andersen, Øivind |
title |
The hemoglobin Gly16β1Asp polymorphism in turbot (Scophthalmus maximus) is differentially distributed across European populations |
title_short |
The hemoglobin Gly16β1Asp polymorphism in turbot (Scophthalmus maximus) is differentially distributed across European populations |
title_full |
The hemoglobin Gly16β1Asp polymorphism in turbot (Scophthalmus maximus) is differentially distributed across European populations |
title_fullStr |
The hemoglobin Gly16β1Asp polymorphism in turbot (Scophthalmus maximus) is differentially distributed across European populations |
title_full_unstemmed |
The hemoglobin Gly16β1Asp polymorphism in turbot (Scophthalmus maximus) is differentially distributed across European populations |
title_sort |
hemoglobin gly16β1asp polymorphism in turbot (scophthalmus maximus) is differentially distributed across european populations |
publisher |
Springer |
url |
http://hdl.handle.net/10347/24213 https://doi.org/10.1007/s10695-020-00872-y |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
https://doi.org/10.1007/s10695-020-00872-y info:eu-repo/grantAgreement/EC/FP7/311920 info:eu-repo/grantAgreement/EC/FP7/613611 Andersen, Ø., Rubiolo, J.A., De Rosa, M.C. et al. The hemoglobin Gly16β1Asp polymorphism in turbot (Scophthalmus maximus) is differentially distributed across European populations. Fish Physiol Biochem 46, 2367–2376 (2020). https://doi-org.ezbusc.usc.gal/10.1007/s10695-020-00872-y 0920-1742 http://hdl.handle.net/10347/24213 doi:10.1007/s10695-020-00872-y 1573-5168 |
op_rights |
© The Author(s) 2020. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1007/s10695-020-00872-y |
container_title |
Fish Physiology and Biochemistry |
container_volume |
46 |
container_issue |
6 |
container_start_page |
2367 |
op_container_end_page |
2376 |
_version_ |
1772819530623483904 |
spelling |
ftunivsantcomp:oai:minerva.usc.es:10347/24213 2023-07-30T04:06:41+02:00 The hemoglobin Gly16β1Asp polymorphism in turbot (Scophthalmus maximus) is differentially distributed across European populations Andersen, Øivind Rubiolo Gaytán, Juan Andrés Rosa, Maria Cristina de Martínez Portela, Paulino Universidade de Santiago de Compostela. Departamento de Zooloxía, Xenética e Antropoloxía Física application/pdf http://hdl.handle.net/10347/24213 https://doi.org/10.1007/s10695-020-00872-y eng eng Springer https://doi.org/10.1007/s10695-020-00872-y info:eu-repo/grantAgreement/EC/FP7/311920 info:eu-repo/grantAgreement/EC/FP7/613611 Andersen, Ø., Rubiolo, J.A., De Rosa, M.C. et al. The hemoglobin Gly16β1Asp polymorphism in turbot (Scophthalmus maximus) is differentially distributed across European populations. Fish Physiol Biochem 46, 2367–2376 (2020). https://doi-org.ezbusc.usc.gal/10.1007/s10695-020-00872-y 0920-1742 http://hdl.handle.net/10347/24213 doi:10.1007/s10695-020-00872-y 1573-5168 © The Author(s) 2020. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Turbot Hemoglobin Polymorphism Body growth Genetic variation Adaptation info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion ftunivsantcomp https://doi.org/10.1007/s10695-020-00872-y 2023-07-11T23:26:08Z Turbot is an important flatfish widely distributed along the European coasts, whose fishery is centered in the North Sea. The commercial value of the species has boosted a successful aquaculture sector in Europe and China. Body growth is the main target of turbot breeding programs and is also a key trait related to local adaptation to temperature and salinity. Differences in growth rate and optimal growth temperature in turbot have been shown to be associated with a hemoglobin polymorphism reported more than 50 years ago. Here, we identified a Gly16Asp amino acid substitution in the β1 globin subunit by searching for genetic variation in the five functional globin genes within the whole annotated turbot genome. We predicted increased stability of the turbot hemoglobin by the replacement of the conserved Gly with the negative charged Asp residue that is consistent with the higher rate of αβ dimer assembly in the human J-Baltimore Gly16β->Asp mutant than in normal HbA. The turbot Hbβ1-Gly16 variant dominated in the northern populations examined, particularly in the Baltic Sea, while the Asp allele showed elevated frequencies in southern populations and was the prevalent variant in the Adriatic Sea. Body weight did not associate with the Hbβ1 genotypes at farming conditions (i.e., high oxygen levels, feeding ad libitum) after analyzing 90 fish with high growth dispersal from nine turbot families. Nevertheless, all data at hand suggest that the turbot hemoglobin polymorphism has an adaptive significance in the variable wild conditions regarding temperature and oxygen availability Open Access funding provided by Norwegian University of Life Sciences. This work was supported by the EU projects AQUATRACE (no. 311920) and FISHBOOST (no. 613611) SI Article in Journal/Newspaper Scophthalmus maximus Turbot Minerva - Repositorio institucional da Universidade de Santiago de Compostela (USC) Fish Physiology and Biochemistry 46 6 2367 2376 |