Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment nextgeneration sequencing strategy
Infectious agents such as the bacteria Vibrio aestuarianus or Ostreid herpesvirus 1 have been repeatedly associated with dramatic disease outbreaks of Crassostrea gigas beds in Europe. Beside roles played by these pathogens, microbial infections in C. gigas may derive from the contribution of a larg...
Published in: | Environmental Microbiology |
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Online Access: | http://hdl.handle.net/10347/21398 https://doi.org/10.1111/1462-2920.14750 |
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ftunivsantcomp:oai:minerva.usc.es:10347/21398 2023-07-30T04:03:05+02:00 Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment nextgeneration sequencing strategy Lasa González, Aide Cesare, Andrea di Tassistro, Giovanni Borello, Alessio Furones, Dolors Carrasco, Noelia Cheslett, Deborah Brechon, Amanda Paillard, Christine Bidault, Adeline Pernet, Fabrice Canesi, Laura Edomi, Paolo Pallavicini, Alberto Pruzzo, Carla Vezzulli, Luigi Universidade de Santiago de Compostela. Departamento de Microbioloxía e Parasitoloxía application/pdf http://hdl.handle.net/10347/21398 https://doi.org/10.1111/1462-2920.14750 eng eng Wiley https://doi.org/10.1111/1462-2920.14750 info:eu-repo/grantAgreement/EC/H2020/678589 Lasa, A., di Cesare, A., Tassistro, G., Borello, A., Gualdi, S., Furones, D., Carrasco, N., Cheslett, D., Brechon, A., Paillard, C., Bidault, A., Pernet, F., Canesi, L., Edomi, P., Pallavicini, A., Pruzzo, C. and Vezzulli, L. (2019), Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment next‐generation sequencing strategy. Environ Microbiol, 21: 4548-4562. doi:10.1111/1462-2920.14750 1462-2912 http://hdl.handle.net/10347/21398 doi:10.1111/1462-2920.14750 1462-2920 © 2019 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. https://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion ftunivsantcomp https://doi.org/10.1111/1462-2920.14750 2023-07-11T23:26:24Z Infectious agents such as the bacteria Vibrio aestuarianus or Ostreid herpesvirus 1 have been repeatedly associated with dramatic disease outbreaks of Crassostrea gigas beds in Europe. Beside roles played by these pathogens, microbial infections in C. gigas may derive from the contribution of a larger number of microorganisms than previously thought, according to an emerging view supporting the polymicrobial nature of bivalve diseases. In this study, the microbial communities associated with a large number of C. gigas samples collected during recurrent mortality episodes at different European sites were investigated by real‐time PCR and 16SrRNA gene‐based microbial profiling. A new target enrichment next‐generation sequencing protocol for selective capturing of 884 phylogenetic and virulence markers of the potential microbial pathogenic community in oyster tissue was developed allowing high taxonomic resolution analysis of the bivalve pathobiota. Comparative analysis of contrasting C. gigas samples conducted using these methods revealed that oyster experiencing mortality outbreaks displayed signs of microbiota disruption associated with the presence of previously undetected potential pathogenic microbial species mostly belonging to genus Vibrio and Arcobacter. The role of these species and their consortia should be targeted by future studies aiming to shed light on mechanisms underlying polymicrobial infections in C. gigas. This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the EU project H2020 VIVALDI Grant No. 678589. We thank all the producers and people helping with sampling collection at the farming sites. We also thank the Ifremer ECOSCOPA network for producing and maintaining the oysters in the Bay of Brest. SI Article in Journal/Newspaper Crassostrea gigas Pacific oyster Minerva - Repositorio institucional da Universidade de Santiago de Compostela (USC) Pacific Environmental Microbiology 21 12 4548 4562 |
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Open Polar |
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Minerva - Repositorio institucional da Universidade de Santiago de Compostela (USC) |
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ftunivsantcomp |
language |
English |
description |
Infectious agents such as the bacteria Vibrio aestuarianus or Ostreid herpesvirus 1 have been repeatedly associated with dramatic disease outbreaks of Crassostrea gigas beds in Europe. Beside roles played by these pathogens, microbial infections in C. gigas may derive from the contribution of a larger number of microorganisms than previously thought, according to an emerging view supporting the polymicrobial nature of bivalve diseases. In this study, the microbial communities associated with a large number of C. gigas samples collected during recurrent mortality episodes at different European sites were investigated by real‐time PCR and 16SrRNA gene‐based microbial profiling. A new target enrichment next‐generation sequencing protocol for selective capturing of 884 phylogenetic and virulence markers of the potential microbial pathogenic community in oyster tissue was developed allowing high taxonomic resolution analysis of the bivalve pathobiota. Comparative analysis of contrasting C. gigas samples conducted using these methods revealed that oyster experiencing mortality outbreaks displayed signs of microbiota disruption associated with the presence of previously undetected potential pathogenic microbial species mostly belonging to genus Vibrio and Arcobacter. The role of these species and their consortia should be targeted by future studies aiming to shed light on mechanisms underlying polymicrobial infections in C. gigas. This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the EU project H2020 VIVALDI Grant No. 678589. We thank all the producers and people helping with sampling collection at the farming sites. We also thank the Ifremer ECOSCOPA network for producing and maintaining the oysters in the Bay of Brest. SI |
author2 |
Universidade de Santiago de Compostela. Departamento de Microbioloxía e Parasitoloxía |
format |
Article in Journal/Newspaper |
author |
Lasa González, Aide Cesare, Andrea di Tassistro, Giovanni Borello, Alessio Furones, Dolors Carrasco, Noelia Cheslett, Deborah Brechon, Amanda Paillard, Christine Bidault, Adeline Pernet, Fabrice Canesi, Laura Edomi, Paolo Pallavicini, Alberto Pruzzo, Carla Vezzulli, Luigi |
spellingShingle |
Lasa González, Aide Cesare, Andrea di Tassistro, Giovanni Borello, Alessio Furones, Dolors Carrasco, Noelia Cheslett, Deborah Brechon, Amanda Paillard, Christine Bidault, Adeline Pernet, Fabrice Canesi, Laura Edomi, Paolo Pallavicini, Alberto Pruzzo, Carla Vezzulli, Luigi Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment nextgeneration sequencing strategy |
author_facet |
Lasa González, Aide Cesare, Andrea di Tassistro, Giovanni Borello, Alessio Furones, Dolors Carrasco, Noelia Cheslett, Deborah Brechon, Amanda Paillard, Christine Bidault, Adeline Pernet, Fabrice Canesi, Laura Edomi, Paolo Pallavicini, Alberto Pruzzo, Carla Vezzulli, Luigi |
author_sort |
Lasa González, Aide |
title |
Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment nextgeneration sequencing strategy |
title_short |
Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment nextgeneration sequencing strategy |
title_full |
Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment nextgeneration sequencing strategy |
title_fullStr |
Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment nextgeneration sequencing strategy |
title_full_unstemmed |
Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment nextgeneration sequencing strategy |
title_sort |
dynamics of the pacific oyster pathobiota during mortality episodes in europe assessed by 16s rrna gene profiling and a new target enrichment nextgeneration sequencing strategy |
publisher |
Wiley |
url |
http://hdl.handle.net/10347/21398 https://doi.org/10.1111/1462-2920.14750 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
https://doi.org/10.1111/1462-2920.14750 info:eu-repo/grantAgreement/EC/H2020/678589 Lasa, A., di Cesare, A., Tassistro, G., Borello, A., Gualdi, S., Furones, D., Carrasco, N., Cheslett, D., Brechon, A., Paillard, C., Bidault, A., Pernet, F., Canesi, L., Edomi, P., Pallavicini, A., Pruzzo, C. and Vezzulli, L. (2019), Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment next‐generation sequencing strategy. Environ Microbiol, 21: 4548-4562. doi:10.1111/1462-2920.14750 1462-2912 http://hdl.handle.net/10347/21398 doi:10.1111/1462-2920.14750 1462-2920 |
op_rights |
© 2019 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. https://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1111/1462-2920.14750 |
container_title |
Environmental Microbiology |
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21 |
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12 |
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4548 |
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4562 |
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1772814025105604608 |