Impact of human-associated Escherichia coli clonal groups in Antarctic pinnipeds: presence of ST73, ST95, ST141 and ST131

There is growing concern about the spreading of human microorganisms in relatively untouched ecosystems such as the Antarctic region. For this reason, three pinniped species (Leptonychotes weddellii, Mirounga leonina and Arctocephalus gazella) from the west coast of the Antartic Peninsula were analy...

Full description

Bibliographic Details
Published in:Scientific Reports
Main Authors: Mora, Azucena, García Peña, Francisco Javier, Alonso, María Pilar, Pedraza Diaz, Susana, Ortega Mora, Luis Miguel, García Parraga, Daniel, López Capón, Cecilia, Viso González, Susana, Dahbi Zbiti, Ghizlane, Marzoa Fandiño, Juan, Sergeant, Martin J., García Menéndez, Vanesa, Blanco Álvarez, Jorge
Other Authors: Universidade de Santiago de Compostela. Departamento de Microbioloxía e Parasitoloxía
Format: Article in Journal/Newspaper
Language:English
Published: Springer Nature
Subjects:
Diseases
Pathogens
Antarctic
The Antarctic
Antarc*
antartic*
Arctocephalus gazella
Mirounga leonina
Online Access:http://hdl.handle.net/10347/17639
https://doi.org/10.1038/s41598-018-22943-0
id ftunivsantcomp:oai:minerva.usc.es:10347/17639
record_format openpolar
institution Open Polar
collection Minerva - Repositorio institucional da Universidade de Santiago de Compostela (USC)
op_collection_id ftunivsantcomp
language English
topic Diseases
Pathogens
spellingShingle Diseases
Pathogens
Mora, Azucena
García Peña, Francisco Javier
Alonso, María Pilar
Pedraza Diaz, Susana
Ortega Mora, Luis Miguel
García Parraga, Daniel
López Capón, Cecilia
Viso González, Susana
Dahbi Zbiti, Ghizlane
Marzoa Fandiño, Juan
Sergeant, Martin J.
García Menéndez, Vanesa
Blanco Álvarez, Jorge
Impact of human-associated Escherichia coli clonal groups in Antarctic pinnipeds: presence of ST73, ST95, ST141 and ST131
topic_facet Diseases
Pathogens
description There is growing concern about the spreading of human microorganisms in relatively untouched ecosystems such as the Antarctic region. For this reason, three pinniped species (Leptonychotes weddellii, Mirounga leonina and Arctocephalus gazella) from the west coast of the Antartic Peninsula were analysed for the presence of Escherichia spp. with the recovery of 158 E. coli and three E. albertii isolates. From those, 23 harboured different eae variants (α1, β1, β2, ε1, θ1, κ, ο), including a bfpA-positive isolate (O49:H10-A-ST206, eae-k) classified as typical enteropathogenic E. coli. Noteworthy, 62 of the 158 E. coli isolates (39.2%) exhibited the ExPEC status and 27 (17.1%) belonged to sequence types (ST) frequently occurring among urinary/bacteremia ExPEC clones: ST12, ST73, ST95, ST131 and ST141. We found similarities >85% within the PFGE-macrorrestriction profiles of pinniped and human clinic O2:H6-B2-ST141 and O16:H5/O25b:H4-B2-ST131 isolates. The in silico analysis of ST131 Cplx genomes from the three pinnipeds (five O25:H4-ST131/PST43-fimH22-virotype D; one O16:H5-ST131/PST506-fimH41; one O25:H4-ST6252/PST9-fimH22-virotype D1) identified IncF and IncI1 plasmids and revealed high core-genome similarities between pinniped and human isolates (H22 and H41 subclones). This is the first study to demonstrate the worrisome presence of human-associated E. coli clonal groups, including ST131, in Antarctic pinnipeds The sampling was funded by the Spanish Ministry of Science and Innovation (CGL-2005-25073-E/ANT and CTM2008-00570) and the Sea World & Bush Gardens Conservation Fund. Work at USC-LREC was supported by projects AGL2013-47852-R from the Ministerio de Economía y Competitividad (MINECO, Spain) and Fondo Europeo de Desarrollo Regional (FEDER); AGL2016-79343-R from the Agencia Estatal de Investigación (AEI, Spain) and FEDER; PI16/01477 from Plan Estatal de I + D + I 2013-2016, Instituto de Salud Carlos III (ISCIII), Subdirección General de Evaluación y Fomento de la Investigación, and FEDER; CN2012/303 ...
author2 Universidade de Santiago de Compostela. Departamento de Microbioloxía e Parasitoloxía
format Article in Journal/Newspaper
author Mora, Azucena
García Peña, Francisco Javier
Alonso, María Pilar
Pedraza Diaz, Susana
Ortega Mora, Luis Miguel
García Parraga, Daniel
López Capón, Cecilia
Viso González, Susana
Dahbi Zbiti, Ghizlane
Marzoa Fandiño, Juan
Sergeant, Martin J.
García Menéndez, Vanesa
Blanco Álvarez, Jorge
author_facet Mora, Azucena
García Peña, Francisco Javier
Alonso, María Pilar
Pedraza Diaz, Susana
Ortega Mora, Luis Miguel
García Parraga, Daniel
López Capón, Cecilia
Viso González, Susana
Dahbi Zbiti, Ghizlane
Marzoa Fandiño, Juan
Sergeant, Martin J.
García Menéndez, Vanesa
Blanco Álvarez, Jorge
author_sort Mora, Azucena
title Impact of human-associated Escherichia coli clonal groups in Antarctic pinnipeds: presence of ST73, ST95, ST141 and ST131
title_short Impact of human-associated Escherichia coli clonal groups in Antarctic pinnipeds: presence of ST73, ST95, ST141 and ST131
title_full Impact of human-associated Escherichia coli clonal groups in Antarctic pinnipeds: presence of ST73, ST95, ST141 and ST131
title_fullStr Impact of human-associated Escherichia coli clonal groups in Antarctic pinnipeds: presence of ST73, ST95, ST141 and ST131
title_full_unstemmed Impact of human-associated Escherichia coli clonal groups in Antarctic pinnipeds: presence of ST73, ST95, ST141 and ST131
title_sort impact of human-associated escherichia coli clonal groups in antarctic pinnipeds: presence of st73, st95, st141 and st131
publisher Springer Nature
url http://hdl.handle.net/10347/17639
https://doi.org/10.1038/s41598-018-22943-0
geographic Antarctic
The Antarctic
geographic_facet Antarctic
The Antarctic
genre Antarc*
Antarctic
antartic*
Arctocephalus gazella
Mirounga leonina
genre_facet Antarc*
Antarctic
antartic*
Arctocephalus gazella
Mirounga leonina
op_relation https://doi.org/10.1038/s41598-018-22943-0
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2013-47852-R/ES/FUENTES DE TRANSMISION DEL GRUPO CLONAL PANDEMICO ST131 DE ESCHERICHIA COLI. CARACTERIZACION MOLECULAR DEL PATOGENO Y DE SU VIRULENCIA IN VIVO, EVALUACION DE RIESGO Y CONTROL
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2016-79343-R/ES
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/PI16-01477/ES/Caracterización molecular de clones de Escherichia coli de alto riesgo (ST131 y otros) causantes de infecciones extraintestinales en seres humanos. Los animales como reservorio
Mora, A., García-Peña, F., Alonso, M., Pedraza-Diaz, S., Ortega-Mora, L., & Garcia-Parraga, D. et al. (2018). Impact of human-associated Escherichia coli clonal groups in Antarctic pinnipeds: presence of ST73, ST95, ST141 and ST131. Scientific Reports, 8(1). doi:10.1038/s41598-018-22943-0
http://hdl.handle.net/10347/17639
doi:10.1038/s41598-018-22943-0
2045-2322
op_rights © Te Author(s) 2018. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
op_doi https://doi.org/10.1038/s41598-018-22943-0
container_title Scientific Reports
container_volume 8
container_issue 1
_version_ 1772821191089717248
spelling ftunivsantcomp:oai:minerva.usc.es:10347/17639 2023-07-30T03:58:22+02:00 Impact of human-associated Escherichia coli clonal groups in Antarctic pinnipeds: presence of ST73, ST95, ST141 and ST131 Mora, Azucena García Peña, Francisco Javier Alonso, María Pilar Pedraza Diaz, Susana Ortega Mora, Luis Miguel García Parraga, Daniel López Capón, Cecilia Viso González, Susana Dahbi Zbiti, Ghizlane Marzoa Fandiño, Juan Sergeant, Martin J. García Menéndez, Vanesa Blanco Álvarez, Jorge Universidade de Santiago de Compostela. Departamento de Microbioloxía e Parasitoloxía application/pdf http://hdl.handle.net/10347/17639 https://doi.org/10.1038/s41598-018-22943-0 eng eng Springer Nature https://doi.org/10.1038/s41598-018-22943-0 info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2013-47852-R/ES/FUENTES DE TRANSMISION DEL GRUPO CLONAL PANDEMICO ST131 DE ESCHERICHIA COLI. CARACTERIZACION MOLECULAR DEL PATOGENO Y DE SU VIRULENCIA IN VIVO, EVALUACION DE RIESGO Y CONTROL info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2016-79343-R/ES info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/PI16-01477/ES/Caracterización molecular de clones de Escherichia coli de alto riesgo (ST131 y otros) causantes de infecciones extraintestinales en seres humanos. Los animales como reservorio Mora, A., García-Peña, F., Alonso, M., Pedraza-Diaz, S., Ortega-Mora, L., & Garcia-Parraga, D. et al. (2018). Impact of human-associated Escherichia coli clonal groups in Antarctic pinnipeds: presence of ST73, ST95, ST141 and ST131. Scientific Reports, 8(1). doi:10.1038/s41598-018-22943-0 http://hdl.handle.net/10347/17639 doi:10.1038/s41598-018-22943-0 2045-2322 © Te Author(s) 2018. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Diseases Pathogens info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion ftunivsantcomp https://doi.org/10.1038/s41598-018-22943-0 2023-07-11T23:26:02Z There is growing concern about the spreading of human microorganisms in relatively untouched ecosystems such as the Antarctic region. For this reason, three pinniped species (Leptonychotes weddellii, Mirounga leonina and Arctocephalus gazella) from the west coast of the Antartic Peninsula were analysed for the presence of Escherichia spp. with the recovery of 158 E. coli and three E. albertii isolates. From those, 23 harboured different eae variants (α1, β1, β2, ε1, θ1, κ, ο), including a bfpA-positive isolate (O49:H10-A-ST206, eae-k) classified as typical enteropathogenic E. coli. Noteworthy, 62 of the 158 E. coli isolates (39.2%) exhibited the ExPEC status and 27 (17.1%) belonged to sequence types (ST) frequently occurring among urinary/bacteremia ExPEC clones: ST12, ST73, ST95, ST131 and ST141. We found similarities >85% within the PFGE-macrorrestriction profiles of pinniped and human clinic O2:H6-B2-ST141 and O16:H5/O25b:H4-B2-ST131 isolates. The in silico analysis of ST131 Cplx genomes from the three pinnipeds (five O25:H4-ST131/PST43-fimH22-virotype D; one O16:H5-ST131/PST506-fimH41; one O25:H4-ST6252/PST9-fimH22-virotype D1) identified IncF and IncI1 plasmids and revealed high core-genome similarities between pinniped and human isolates (H22 and H41 subclones). This is the first study to demonstrate the worrisome presence of human-associated E. coli clonal groups, including ST131, in Antarctic pinnipeds The sampling was funded by the Spanish Ministry of Science and Innovation (CGL-2005-25073-E/ANT and CTM2008-00570) and the Sea World & Bush Gardens Conservation Fund. Work at USC-LREC was supported by projects AGL2013-47852-R from the Ministerio de Economía y Competitividad (MINECO, Spain) and Fondo Europeo de Desarrollo Regional (FEDER); AGL2016-79343-R from the Agencia Estatal de Investigación (AEI, Spain) and FEDER; PI16/01477 from Plan Estatal de I + D + I 2013-2016, Instituto de Salud Carlos III (ISCIII), Subdirección General de Evaluación y Fomento de la Investigación, and FEDER; CN2012/303 ... Article in Journal/Newspaper Antarc* Antarctic antartic* Arctocephalus gazella Mirounga leonina Minerva - Repositorio institucional da Universidade de Santiago de Compostela (USC) Antarctic The Antarctic Scientific Reports 8 1

Similar Items