Citrate carrier activity and cardiolipin level in eel (Anguilla anguilla) liver mitochondria
The activity of the tricarboxylate (citrate) carrier has been assayed in intact liver mitochondria from yellow eel (Anguilla anguilla) and compared to that from rat. The eel-citrate carrier specific activity was approximately 1.7-fold higher than that assayed in rat-liver mitochondria. The content o...
Published in: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
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ftunivsalento:oai:iris.unisalento.it:11587/515415 2024-04-28T07:55:03+00:00 Citrate carrier activity and cardiolipin level in eel (Anguilla anguilla) liver mitochondria Giudetti A. M. Siculella L. Gnoni G. V. Giudetti, A. M. Siculella, L. Gnoni, G. V. 2002 ELETTRONICO https://hdl.handle.net/11587/515415 https://doi.org/10.1016/S1096-4959(02)00128-8 eng eng volume:133 firstpage:227 lastpage:234 numberofpages:8 journal:COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY. PART B, BIOCHEMISTRY & MOLECULAR BIOLOGY https://hdl.handle.net/11587/515415 doi:10.1016/S1096-4959(02)00128-8 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0036791155 Cardiolipin Citrate carrier Eel Fatty acid Lipogenesi Liver Phospholipid Rat info:eu-repo/semantics/article 2002 ftunivsalento https://doi.org/10.1016/S1096-4959(02)00128-8 2024-04-11T00:23:13Z The activity of the tricarboxylate (citrate) carrier has been assayed in intact liver mitochondria from yellow eel (Anguilla anguilla) and compared to that from rat. The eel-citrate carrier specific activity was approximately 1.7-fold higher than that assayed in rat-liver mitochondria. The content of the main mitochondrial phospholipids, phosphatidylethanolamine and phosphatidylcholine, did not show a significant difference between the two species, while in eel a higher cardiolipin level was observed. Fatty acid composition of eel-liver mitochondrial phospholipids was characterised by a large amount of unsaturated fatty acids, dominated by octadecaenoic acid (C18:1n-9) and docosahexaenoic acid (C22:6n-3). The cardiolipin fatty acid pattern of eel-liver mitochondria showed, with respect to the rat, a higher C20:5n-3 and C22:6n-3 content and a lower amount of C18:2n-6 and C20:4n-6. A noticeable activity of lipogenic enzymes was also detected in eel liver cytosol. The results of this study suggest that the remarkable activity of the citrate carrier in eel-liver mitochondria can most likely be ascribed to a considerable cardiolipin level. A covariance of citrate carrier and lipogenic enzyme activities was observed. © 2002 Elsevier Science Inc. All rights reserved. Article in Journal/Newspaper Anguilla anguilla Università del Salento: CINECA IRIS Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 133 2 227 234 |
institution |
Open Polar |
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Università del Salento: CINECA IRIS |
op_collection_id |
ftunivsalento |
language |
English |
topic |
Cardiolipin Citrate carrier Eel Fatty acid Lipogenesi Liver Phospholipid Rat |
spellingShingle |
Cardiolipin Citrate carrier Eel Fatty acid Lipogenesi Liver Phospholipid Rat Giudetti A. M. Siculella L. Gnoni G. V. Citrate carrier activity and cardiolipin level in eel (Anguilla anguilla) liver mitochondria |
topic_facet |
Cardiolipin Citrate carrier Eel Fatty acid Lipogenesi Liver Phospholipid Rat |
description |
The activity of the tricarboxylate (citrate) carrier has been assayed in intact liver mitochondria from yellow eel (Anguilla anguilla) and compared to that from rat. The eel-citrate carrier specific activity was approximately 1.7-fold higher than that assayed in rat-liver mitochondria. The content of the main mitochondrial phospholipids, phosphatidylethanolamine and phosphatidylcholine, did not show a significant difference between the two species, while in eel a higher cardiolipin level was observed. Fatty acid composition of eel-liver mitochondrial phospholipids was characterised by a large amount of unsaturated fatty acids, dominated by octadecaenoic acid (C18:1n-9) and docosahexaenoic acid (C22:6n-3). The cardiolipin fatty acid pattern of eel-liver mitochondria showed, with respect to the rat, a higher C20:5n-3 and C22:6n-3 content and a lower amount of C18:2n-6 and C20:4n-6. A noticeable activity of lipogenic enzymes was also detected in eel liver cytosol. The results of this study suggest that the remarkable activity of the citrate carrier in eel-liver mitochondria can most likely be ascribed to a considerable cardiolipin level. A covariance of citrate carrier and lipogenic enzyme activities was observed. © 2002 Elsevier Science Inc. All rights reserved. |
author2 |
Giudetti, A. M. Siculella, L. Gnoni, G. V. |
format |
Article in Journal/Newspaper |
author |
Giudetti A. M. Siculella L. Gnoni G. V. |
author_facet |
Giudetti A. M. Siculella L. Gnoni G. V. |
author_sort |
Giudetti A. M. |
title |
Citrate carrier activity and cardiolipin level in eel (Anguilla anguilla) liver mitochondria |
title_short |
Citrate carrier activity and cardiolipin level in eel (Anguilla anguilla) liver mitochondria |
title_full |
Citrate carrier activity and cardiolipin level in eel (Anguilla anguilla) liver mitochondria |
title_fullStr |
Citrate carrier activity and cardiolipin level in eel (Anguilla anguilla) liver mitochondria |
title_full_unstemmed |
Citrate carrier activity and cardiolipin level in eel (Anguilla anguilla) liver mitochondria |
title_sort |
citrate carrier activity and cardiolipin level in eel (anguilla anguilla) liver mitochondria |
publishDate |
2002 |
url |
https://hdl.handle.net/11587/515415 https://doi.org/10.1016/S1096-4959(02)00128-8 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
volume:133 firstpage:227 lastpage:234 numberofpages:8 journal:COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY. PART B, BIOCHEMISTRY & MOLECULAR BIOLOGY https://hdl.handle.net/11587/515415 doi:10.1016/S1096-4959(02)00128-8 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0036791155 |
op_doi |
https://doi.org/10.1016/S1096-4959(02)00128-8 |
container_title |
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
container_volume |
133 |
container_issue |
2 |
container_start_page |
227 |
op_container_end_page |
234 |
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