Slc15a1 transporters in teleosts fish: PepT1a and PepT1b, comparative functional studies
Di/tripeptides are key nutrients in animal diets, and fundamental for growth. They are transported across the membrane of enterocytes via the Slc15a1/PepT1 peptide transporter, that uses an inwardly-directed proton electrochemical gradient to drive the uptake. Due to a genome duplication event, PepT...
Main Authors: | , , , , , , , |
---|---|
Other Authors: | |
Format: | Conference Object |
Language: | English |
Published: |
WILEY
2019
|
Subjects: | |
Online Access: | http://hdl.handle.net/11587/442329 |
id |
ftunivsalento:oai:iris.unisalento.it:11587/442329 |
---|---|
record_format |
openpolar |
spelling |
ftunivsalento:oai:iris.unisalento.it:11587/442329 2024-04-14T08:09:18+00:00 Slc15a1 transporters in teleosts fish: PepT1a and PepT1b, comparative functional studies Vacca, F Bossi, E Gomes, AS Cinquetti, R Barca, A Verri, T Murashita, K Ronnestad, I Vacca, F Bossi, E Gomes, A Cinquetti, R Barca, A Verri, T Murashita, K Ronnestad, I 2019 STAMPA http://hdl.handle.net/11587/442329 eng eng WILEY country:USA place:111 RIVER ST, HOBOKEN 07030-5774, NJ USA info:eu-repo/semantics/altIdentifier/wos/WOS:000485252000121 ispartofbook:Special Issue:Joint Meeting of the Federation of European Physiological Societies (FEPS) and the Italian Physiological Society (SIF) Bologna (Italy), September 10th – 13th 2019 FEPS 2019, Joint Meeting of the Federation of European Physiological Societies (FEPS) and the Italian Physiological Society (SIF) volume:227 firstpage:50 lastpage:50 numberofpages:1 http://hdl.handle.net/11587/442329 info:eu-repo/semantics/conferenceObject 2019 ftunivsalento 2024-03-21T18:17:03Z Di/tripeptides are key nutrients in animal diets, and fundamental for growth. They are transported across the membrane of enterocytes via the Slc15a1/PepT1 peptide transporter, that uses an inwardly-directed proton electrochemical gradient to drive the uptake. Due to a genome duplication event, PepT1a and PepT1b paralogues are found in teleost fish. Two PepT1a transporters, respectively cloned from the Atlantic salmon (Salmo salar) and zebrafish (Danio rerio), namely AsPepT1a and zfPepT1a, have been characterized. For both orthologs, function was verified by heterologous expression in Xenopus laevis oocytes, highlighting electrogenic, Na+-independent and pH-dependent transport similarly to the well-known PepT1b. The transient currents and the transport currents recorded from AsPept1a and zfPepT1a indicate significant functional differences with respect to PepT1b. PepT1a can be described as a low‐affinity/high‐capacity system, but its substrates preference profile is peculiar in the species, particularly for charged dipeptides. Moreover, the pre-steady state (PSS) currents, that reflect the first steps of transport cycle display in the charge/voltage relationship differences with respect to Pept1b in the voltage dependence. Considering that the PSS are consequence of the rearrangement of the protein in the membrane electric field, PepT1a transporters interact with the substrate differently from PepT1b. In addition, PepT1a and PepT1b have similar expression profile but different expression levels. These data together with a significant dissimilar substrate specificity, support the idea of distinct roles for these proteins in peptide recognition and transport. Conference Object Atlantic salmon Salmo salar Università del Salento: CINECA IRIS |
institution |
Open Polar |
collection |
Università del Salento: CINECA IRIS |
op_collection_id |
ftunivsalento |
language |
English |
description |
Di/tripeptides are key nutrients in animal diets, and fundamental for growth. They are transported across the membrane of enterocytes via the Slc15a1/PepT1 peptide transporter, that uses an inwardly-directed proton electrochemical gradient to drive the uptake. Due to a genome duplication event, PepT1a and PepT1b paralogues are found in teleost fish. Two PepT1a transporters, respectively cloned from the Atlantic salmon (Salmo salar) and zebrafish (Danio rerio), namely AsPepT1a and zfPepT1a, have been characterized. For both orthologs, function was verified by heterologous expression in Xenopus laevis oocytes, highlighting electrogenic, Na+-independent and pH-dependent transport similarly to the well-known PepT1b. The transient currents and the transport currents recorded from AsPept1a and zfPepT1a indicate significant functional differences with respect to PepT1b. PepT1a can be described as a low‐affinity/high‐capacity system, but its substrates preference profile is peculiar in the species, particularly for charged dipeptides. Moreover, the pre-steady state (PSS) currents, that reflect the first steps of transport cycle display in the charge/voltage relationship differences with respect to Pept1b in the voltage dependence. Considering that the PSS are consequence of the rearrangement of the protein in the membrane electric field, PepT1a transporters interact with the substrate differently from PepT1b. In addition, PepT1a and PepT1b have similar expression profile but different expression levels. These data together with a significant dissimilar substrate specificity, support the idea of distinct roles for these proteins in peptide recognition and transport. |
author2 |
Vacca, F Bossi, E Gomes, A Cinquetti, R Barca, A Verri, T Murashita, K Ronnestad, I |
format |
Conference Object |
author |
Vacca, F Bossi, E Gomes, AS Cinquetti, R Barca, A Verri, T Murashita, K Ronnestad, I |
spellingShingle |
Vacca, F Bossi, E Gomes, AS Cinquetti, R Barca, A Verri, T Murashita, K Ronnestad, I Slc15a1 transporters in teleosts fish: PepT1a and PepT1b, comparative functional studies |
author_facet |
Vacca, F Bossi, E Gomes, AS Cinquetti, R Barca, A Verri, T Murashita, K Ronnestad, I |
author_sort |
Vacca, F |
title |
Slc15a1 transporters in teleosts fish: PepT1a and PepT1b, comparative functional studies |
title_short |
Slc15a1 transporters in teleosts fish: PepT1a and PepT1b, comparative functional studies |
title_full |
Slc15a1 transporters in teleosts fish: PepT1a and PepT1b, comparative functional studies |
title_fullStr |
Slc15a1 transporters in teleosts fish: PepT1a and PepT1b, comparative functional studies |
title_full_unstemmed |
Slc15a1 transporters in teleosts fish: PepT1a and PepT1b, comparative functional studies |
title_sort |
slc15a1 transporters in teleosts fish: pept1a and pept1b, comparative functional studies |
publisher |
WILEY |
publishDate |
2019 |
url |
http://hdl.handle.net/11587/442329 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000485252000121 ispartofbook:Special Issue:Joint Meeting of the Federation of European Physiological Societies (FEPS) and the Italian Physiological Society (SIF) Bologna (Italy), September 10th – 13th 2019 FEPS 2019, Joint Meeting of the Federation of European Physiological Societies (FEPS) and the Italian Physiological Society (SIF) volume:227 firstpage:50 lastpage:50 numberofpages:1 http://hdl.handle.net/11587/442329 |
_version_ |
1796306785274953728 |