Ultrastructure, chemical composition and biosynthesis of cell wall polysaccharides in Koliella antarctica (Klebsormidiales, Chlorophyta)
The chemical composition, biosynthesis and ultrastructural organization of the cell wall of cultures of Koliella antarctica (Klebsormidiales, Chlorophyta), a green microalga obtained from samples of sea water taken near the Italian Station of Terra Nova (Ross Sea) during the austral summer 1989-1990...
Published in: | European Journal of Phycology |
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Online Access: | http://hdl.handle.net/11587/369416 https://doi.org/10.1080/09670260010001735931 |
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ftunivsalento:oai:iris.unisalento.it:11587/369416 2024-04-21T07:49:56+00:00 Ultrastructure, chemical composition and biosynthesis of cell wall polysaccharides in Koliella antarctica (Klebsormidiales, Chlorophyta) PIRO, Gabriella LENUCCI, Marcello Salvatore DALESSANDRO, Giuseppe LA ROCCA N. RASCIO N. MORO I. ANDREOLI C. Piro, Gabriella Lenucci, Marcello Salvatore Dalessandro, Giuseppe LA ROCCA, N. Rascio, N. Moro, I. Andreoli, C. 2000 STAMPA http://hdl.handle.net/11587/369416 https://doi.org/10.1080/09670260010001735931 eng eng info:eu-repo/semantics/altIdentifier/wos/000166621200003 volume:35 firstpage:331 lastpage:337 numberofpages:7 journal:EUROPEAN JOURNAL OF PHYCOLOGY http://hdl.handle.net/11587/369416 doi:10.1080/09670260010001735931 cell wall biosynthesi cell wall polysaccharide Chlorophyta β-1 4-glucan green microalga Koliella antarctica myo-inositol pathway info:eu-repo/semantics/article 2000 ftunivsalento https://doi.org/10.1080/09670260010001735931 2024-03-28T01:42:08Z The chemical composition, biosynthesis and ultrastructural organization of the cell wall of cultures of Koliella antarctica (Klebsormidiales, Chlorophyta), a green microalga obtained from samples of sea water taken near the Italian Station of Terra Nova (Ross Sea) during the austral summer 1989-1990, have been studied. Purified cell walls of the microalga were sequentially treated with chemicals which, in higher plants, solubilize matrix polysaccharides (pectins and hemicelluloses) and leave an insoluble residue considered to be alpha -cellulose. CDTA plus Na2CO3- and KOH-solubilized polysaccharides were made up of glucosyl residues (more than 90 mol%), as well as some minor sugars (mannose and rhamnose). Linkage analysis and enzymic hydrolysis of the matrix polysaccharides indicated the presence of beta -1,4-linked glucans with minor amounts of other uncharacterized polysaccharides. The insoluble residue was composed of a small amount of crystalline alpha -cellulose associated with mannan or glucomannan chains. The biosynthesis of cell wall polysaccharides was studied by incubating the microalgae in the presence of D-[U-C-14]glucose or myo-[U-C-14]inositol. The radioactive glycosyl residues incorporated into the polysaccharides solubilized from purified cell walls of the cultured microalga corresponded to those detected in the chemical analysis of the cell wall. The myo-inositol oxidation pathway was demonstrated to be functional in the microalga. Cell wall ultrastructural observations showed a loose network of cellulose microfibrils in an extended matrix. Article in Journal/Newspaper Antarc* Antarctica Ross Sea Università del Salento: CINECA IRIS European Journal of Phycology 35 4 331 337 |
institution |
Open Polar |
collection |
Università del Salento: CINECA IRIS |
op_collection_id |
ftunivsalento |
language |
English |
topic |
cell wall biosynthesi cell wall polysaccharide Chlorophyta β-1 4-glucan green microalga Koliella antarctica myo-inositol pathway |
spellingShingle |
cell wall biosynthesi cell wall polysaccharide Chlorophyta β-1 4-glucan green microalga Koliella antarctica myo-inositol pathway PIRO, Gabriella LENUCCI, Marcello Salvatore DALESSANDRO, Giuseppe LA ROCCA N. RASCIO N. MORO I. ANDREOLI C. Ultrastructure, chemical composition and biosynthesis of cell wall polysaccharides in Koliella antarctica (Klebsormidiales, Chlorophyta) |
topic_facet |
cell wall biosynthesi cell wall polysaccharide Chlorophyta β-1 4-glucan green microalga Koliella antarctica myo-inositol pathway |
description |
The chemical composition, biosynthesis and ultrastructural organization of the cell wall of cultures of Koliella antarctica (Klebsormidiales, Chlorophyta), a green microalga obtained from samples of sea water taken near the Italian Station of Terra Nova (Ross Sea) during the austral summer 1989-1990, have been studied. Purified cell walls of the microalga were sequentially treated with chemicals which, in higher plants, solubilize matrix polysaccharides (pectins and hemicelluloses) and leave an insoluble residue considered to be alpha -cellulose. CDTA plus Na2CO3- and KOH-solubilized polysaccharides were made up of glucosyl residues (more than 90 mol%), as well as some minor sugars (mannose and rhamnose). Linkage analysis and enzymic hydrolysis of the matrix polysaccharides indicated the presence of beta -1,4-linked glucans with minor amounts of other uncharacterized polysaccharides. The insoluble residue was composed of a small amount of crystalline alpha -cellulose associated with mannan or glucomannan chains. The biosynthesis of cell wall polysaccharides was studied by incubating the microalgae in the presence of D-[U-C-14]glucose or myo-[U-C-14]inositol. The radioactive glycosyl residues incorporated into the polysaccharides solubilized from purified cell walls of the cultured microalga corresponded to those detected in the chemical analysis of the cell wall. The myo-inositol oxidation pathway was demonstrated to be functional in the microalga. Cell wall ultrastructural observations showed a loose network of cellulose microfibrils in an extended matrix. |
author2 |
Piro, Gabriella Lenucci, Marcello Salvatore Dalessandro, Giuseppe LA ROCCA, N. Rascio, N. Moro, I. Andreoli, C. |
format |
Article in Journal/Newspaper |
author |
PIRO, Gabriella LENUCCI, Marcello Salvatore DALESSANDRO, Giuseppe LA ROCCA N. RASCIO N. MORO I. ANDREOLI C. |
author_facet |
PIRO, Gabriella LENUCCI, Marcello Salvatore DALESSANDRO, Giuseppe LA ROCCA N. RASCIO N. MORO I. ANDREOLI C. |
author_sort |
PIRO, Gabriella |
title |
Ultrastructure, chemical composition and biosynthesis of cell wall polysaccharides in Koliella antarctica (Klebsormidiales, Chlorophyta) |
title_short |
Ultrastructure, chemical composition and biosynthesis of cell wall polysaccharides in Koliella antarctica (Klebsormidiales, Chlorophyta) |
title_full |
Ultrastructure, chemical composition and biosynthesis of cell wall polysaccharides in Koliella antarctica (Klebsormidiales, Chlorophyta) |
title_fullStr |
Ultrastructure, chemical composition and biosynthesis of cell wall polysaccharides in Koliella antarctica (Klebsormidiales, Chlorophyta) |
title_full_unstemmed |
Ultrastructure, chemical composition and biosynthesis of cell wall polysaccharides in Koliella antarctica (Klebsormidiales, Chlorophyta) |
title_sort |
ultrastructure, chemical composition and biosynthesis of cell wall polysaccharides in koliella antarctica (klebsormidiales, chlorophyta) |
publishDate |
2000 |
url |
http://hdl.handle.net/11587/369416 https://doi.org/10.1080/09670260010001735931 |
genre |
Antarc* Antarctica Ross Sea |
genre_facet |
Antarc* Antarctica Ross Sea |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/000166621200003 volume:35 firstpage:331 lastpage:337 numberofpages:7 journal:EUROPEAN JOURNAL OF PHYCOLOGY http://hdl.handle.net/11587/369416 doi:10.1080/09670260010001735931 |
op_doi |
https://doi.org/10.1080/09670260010001735931 |
container_title |
European Journal of Phycology |
container_volume |
35 |
container_issue |
4 |
container_start_page |
331 |
op_container_end_page |
337 |
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1796933861148459008 |