Cl-absorption in European eel intestine and its regulation.
The intestinal epithelium of the euryhaline teleost fish, Anguilla anguilla, absorbs Cl-transepithelially. This gives rise to a negative transepithelial potential at the basolateral side of the epithelium and to a measured short circuit current. Cl- absorption occurs via bumetanide-sensitive Na+-K+-...
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Online Access: | http://hdl.handle.net/11587/300514 https://doi.org/10.1002/jez.a.10310 |
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ftunivsalento:oai:iris.unisalento.it:11587/300514 2024-04-14T08:01:03+00:00 Cl-absorption in European eel intestine and its regulation. SCHETTINO, Trifone LIONETTO, Maria Giulia Schettino, Trifone Lionetto, Maria Giulia 2003 STAMPA http://hdl.handle.net/11587/300514 https://doi.org/10.1002/jez.a.10310 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000186292800010 volume:300A firstpage:63 lastpage:68 numberofpages:6 journal:JOURNAL OF EXPERIMENTAL ZOOLOGY. PART A COMPARATIVE EXPERIMENTAL BIOLOGY http://hdl.handle.net/11587/300514 doi:10.1002/jez.a.10310 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0346233563 info:eu-repo/semantics/article 2003 ftunivsalento https://doi.org/10.1002/jez.a.10310 2024-03-21T18:05:39Z The intestinal epithelium of the euryhaline teleost fish, Anguilla anguilla, absorbs Cl-transepithelially. This gives rise to a negative transepithelial potential at the basolateral side of the epithelium and to a measured short circuit current. Cl- absorption occurs via bumetanide-sensitive Na+-K+-2Cl- cotransport, localized on the luminal membrane. The cotransport operates in parallel with a luminal K+ conductance that recycles the ion into the lumen. Cl- leaves the cell across the basolateral membrane by way of Cl- conductance and presumably via a KCl cotransport. The driving force for this process is provided by the electrochemical sodium gradient across the plasma membrane, generated and maintained by the basolateral Na+-K+-ATPase. The resulting NaCl absorption process is active and enables marine fish to take up water, thereby compensating for water that was lost passively from the body. Fresh water acclimatized eel also absorb Cl- actively, although in smaller quantities, utilizing the same ion transport mechanisms as marine eels. This mechanism is basically the same as the model proposed for the thick ascending limb (cTAL). Cl- absorption is regulated by a number of cellular factors, such as HCO3-, pH, Ca2+, cyclic nucleotides, and cytoskeletal elements. It is sensitive to osmotic stress, and therefore is a good physiological model to study ion transport mechanisms that are activated when osmotic stress induces cell volume regulation. The activation of these various ion transport pathways is dependent on cellular transduction mechanisms in which phosphorylation events (mainly by PKC and MLCK for the hypertonic response) and cytoskeletal elements, either microfilaments or microtubules, seem to play key roles. Article in Journal/Newspaper Anguilla anguilla Università del Salento: CINECA IRIS Journal of Experimental Zoology 300A 1 63 68 |
institution |
Open Polar |
collection |
Università del Salento: CINECA IRIS |
op_collection_id |
ftunivsalento |
language |
English |
description |
The intestinal epithelium of the euryhaline teleost fish, Anguilla anguilla, absorbs Cl-transepithelially. This gives rise to a negative transepithelial potential at the basolateral side of the epithelium and to a measured short circuit current. Cl- absorption occurs via bumetanide-sensitive Na+-K+-2Cl- cotransport, localized on the luminal membrane. The cotransport operates in parallel with a luminal K+ conductance that recycles the ion into the lumen. Cl- leaves the cell across the basolateral membrane by way of Cl- conductance and presumably via a KCl cotransport. The driving force for this process is provided by the electrochemical sodium gradient across the plasma membrane, generated and maintained by the basolateral Na+-K+-ATPase. The resulting NaCl absorption process is active and enables marine fish to take up water, thereby compensating for water that was lost passively from the body. Fresh water acclimatized eel also absorb Cl- actively, although in smaller quantities, utilizing the same ion transport mechanisms as marine eels. This mechanism is basically the same as the model proposed for the thick ascending limb (cTAL). Cl- absorption is regulated by a number of cellular factors, such as HCO3-, pH, Ca2+, cyclic nucleotides, and cytoskeletal elements. It is sensitive to osmotic stress, and therefore is a good physiological model to study ion transport mechanisms that are activated when osmotic stress induces cell volume regulation. The activation of these various ion transport pathways is dependent on cellular transduction mechanisms in which phosphorylation events (mainly by PKC and MLCK for the hypertonic response) and cytoskeletal elements, either microfilaments or microtubules, seem to play key roles. |
author2 |
Schettino, Trifone Lionetto, Maria Giulia |
format |
Article in Journal/Newspaper |
author |
SCHETTINO, Trifone LIONETTO, Maria Giulia |
spellingShingle |
SCHETTINO, Trifone LIONETTO, Maria Giulia Cl-absorption in European eel intestine and its regulation. |
author_facet |
SCHETTINO, Trifone LIONETTO, Maria Giulia |
author_sort |
SCHETTINO, Trifone |
title |
Cl-absorption in European eel intestine and its regulation. |
title_short |
Cl-absorption in European eel intestine and its regulation. |
title_full |
Cl-absorption in European eel intestine and its regulation. |
title_fullStr |
Cl-absorption in European eel intestine and its regulation. |
title_full_unstemmed |
Cl-absorption in European eel intestine and its regulation. |
title_sort |
cl-absorption in european eel intestine and its regulation. |
publishDate |
2003 |
url |
http://hdl.handle.net/11587/300514 https://doi.org/10.1002/jez.a.10310 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000186292800010 volume:300A firstpage:63 lastpage:68 numberofpages:6 journal:JOURNAL OF EXPERIMENTAL ZOOLOGY. PART A COMPARATIVE EXPERIMENTAL BIOLOGY http://hdl.handle.net/11587/300514 doi:10.1002/jez.a.10310 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0346233563 |
op_doi |
https://doi.org/10.1002/jez.a.10310 |
container_title |
Journal of Experimental Zoology |
container_volume |
300A |
container_issue |
1 |
container_start_page |
63 |
op_container_end_page |
68 |
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1796306742334717952 |