Brush-border amino acid transport mechanisms in carnivorous eel intestine
Brush-border membrane vesicles (BBMV) were prepared from European eel (Anguilla anguilla) intestinal epithelium by a magnesium-ethylene glycolbis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) precipitation technique. Amino acid transport by these purified vesicle preparations wa...
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ftunivsalento:oai:iris.unisalento.it:11587/105209 2024-04-14T08:01:02+00:00 Brush-border amino acid transport mechanisms in carnivorous eel intestine STORELLI, Carlo VILELLA, Sebastiano MAFFIA, Michele M. P. ROMANO G. CASSANO Storelli, Carlo Vilella, Sebastiano M. P., Romano Maffia, Michele G., Cassano 1989 STAMPA http://hdl.handle.net/11587/105209 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:A1989AQ39200050 volume:257 firstpage:R506 lastpage:R510 numberofpages:5 journal:AMERICAN JOURNAL OF PHYSIOLOGY http://hdl.handle.net/11587/105209 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0024357612 info:eu-repo/semantics/article 1989 ftunivsalento 2024-03-21T18:05:03Z Brush-border membrane vesicles (BBMV) were prepared from European eel (Anguilla anguilla) intestinal epithelium by a magnesium-ethylene glycolbis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) precipitation technique. Amino acid transport by these purified vesicle preparations was investigated using either radiolabeled substrates or the voltage-sensitive fluorescent dye 3,3'-diethylthiadicarbocyanine iodide [DiSC2(5)]. All amino acids tested exhibited carrier-mediated, Na+-dependent and Na+-independent transfer processes plus diffusion. The only exceptions were glutamic acid and proline, which displayed Na+ dependency and diffusion but did not appear to be transported by Na+-independent agencies. Carrier-mediated transport kinetic constants (Kapp and Jmax) for several amino acids are reported. Cis-inhibition experiments suggested the presence of at least four distinct Na+-dependent transport systems in eel intestinal BBMV: 1) an anionic transport process for glutamic and aspartic acids; 2) a cationic mechanism for lysine and arginine; 3) a relatively specific neutral amino acid carrier for proline and alpha-(methylamino)isobutyric acid; and 4) a nonspecific neutral amino acid system for most other substrates of this group. This scheme for carnivorous fish intestine most closely approximates that reported for mammalian gut with minor dissimilarities that may relate to metabolic differences or specific dietary requirements of the two vertebrate groups. Article in Journal/Newspaper Anguilla anguilla Università del Salento: CINECA IRIS |
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Open Polar |
collection |
Università del Salento: CINECA IRIS |
op_collection_id |
ftunivsalento |
language |
English |
description |
Brush-border membrane vesicles (BBMV) were prepared from European eel (Anguilla anguilla) intestinal epithelium by a magnesium-ethylene glycolbis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) precipitation technique. Amino acid transport by these purified vesicle preparations was investigated using either radiolabeled substrates or the voltage-sensitive fluorescent dye 3,3'-diethylthiadicarbocyanine iodide [DiSC2(5)]. All amino acids tested exhibited carrier-mediated, Na+-dependent and Na+-independent transfer processes plus diffusion. The only exceptions were glutamic acid and proline, which displayed Na+ dependency and diffusion but did not appear to be transported by Na+-independent agencies. Carrier-mediated transport kinetic constants (Kapp and Jmax) for several amino acids are reported. Cis-inhibition experiments suggested the presence of at least four distinct Na+-dependent transport systems in eel intestinal BBMV: 1) an anionic transport process for glutamic and aspartic acids; 2) a cationic mechanism for lysine and arginine; 3) a relatively specific neutral amino acid carrier for proline and alpha-(methylamino)isobutyric acid; and 4) a nonspecific neutral amino acid system for most other substrates of this group. This scheme for carnivorous fish intestine most closely approximates that reported for mammalian gut with minor dissimilarities that may relate to metabolic differences or specific dietary requirements of the two vertebrate groups. |
author2 |
Storelli, Carlo Vilella, Sebastiano M. P., Romano Maffia, Michele G., Cassano |
format |
Article in Journal/Newspaper |
author |
STORELLI, Carlo VILELLA, Sebastiano MAFFIA, Michele M. P. ROMANO G. CASSANO |
spellingShingle |
STORELLI, Carlo VILELLA, Sebastiano MAFFIA, Michele M. P. ROMANO G. CASSANO Brush-border amino acid transport mechanisms in carnivorous eel intestine |
author_facet |
STORELLI, Carlo VILELLA, Sebastiano MAFFIA, Michele M. P. ROMANO G. CASSANO |
author_sort |
STORELLI, Carlo |
title |
Brush-border amino acid transport mechanisms in carnivorous eel intestine |
title_short |
Brush-border amino acid transport mechanisms in carnivorous eel intestine |
title_full |
Brush-border amino acid transport mechanisms in carnivorous eel intestine |
title_fullStr |
Brush-border amino acid transport mechanisms in carnivorous eel intestine |
title_full_unstemmed |
Brush-border amino acid transport mechanisms in carnivorous eel intestine |
title_sort |
brush-border amino acid transport mechanisms in carnivorous eel intestine |
publishDate |
1989 |
url |
http://hdl.handle.net/11587/105209 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:A1989AQ39200050 volume:257 firstpage:R506 lastpage:R510 numberofpages:5 journal:AMERICAN JOURNAL OF PHYSIOLOGY http://hdl.handle.net/11587/105209 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0024357612 |
_version_ |
1796306545510711296 |