Incomplete meiotic sex chromosome inactivation in the domestic dog
Background: In mammalian meiotic prophase, homologous chromosome recognition is aided by formation and repair of programmed DNA double-strand breaks (DSBs). Subsequently, stable associations form through homologous chromosome synapsis. In male mouse meiosis, the largely heterologous X and Y chromoso...
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ftunivrotterdam:oai:repub.eur.nl:90020 2023-07-16T03:57:56+02:00 Incomplete meiotic sex chromosome inactivation in the domestic dog Federici, F. (Federica) Mulugeta, E. (Eskeatnaf) Schoenmakers, S. (Sam) Wassenaar, E. (Evelyne) Hoogerbrugge, J.W. (Jos) Heijden, G.W. (Godfried) van der Cappellen, W.A. (Gert) van Slotman, J.A. (Johan A.) IJcken, W.F.J. (Wilfred) van Laven, J.S.E. (Joop) Grootegoed, J.A. (Anton) Baarends, W.M. (Willy) 2015-04-12 application/pdf http://repub.eur.nl/pub/90020 https://doi.org/10.1186/s12864-015-1501-9 en eng http://repub.eur.nl/pub/90020 doi:10.1186/s12864-015-1501-9 urn:hdl:1765/90020 info:eu-repo/semantics/openAccess BMC Genomics vol. 16 no. 1 DNA double-strand break repair Dog Meiosis Meiotic sex chromosome inactivation MSCI Sex chromosomes Spermatids Spermatocytes Spermatogenesis Synaptonemal complex Transcriptome info:eu-repo/semantics/article 2015 ftunivrotterdam https://doi.org/10.1186/s12864-015-1501-9 2023-06-26T22:32:58Z Background: In mammalian meiotic prophase, homologous chromosome recognition is aided by formation and repair of programmed DNA double-strand breaks (DSBs). Subsequently, stable associations form through homologous chromosome synapsis. In male mouse meiosis, the largely heterologous X and Y chromosomes synapse only in their short pseudoautosomal regions (PARs), and DSBs persist along the unsynapsed non-homologous arms of these sex chromosomes. Asynapsis of these arms and the persistent DSBs then trigger transcriptional silencing through meiotic sex chromosome inactivation (MSCI), resulting in formation of the XY body. This inactive state is partially maintained in post-meiotic haploid spermatids (postmeiotic sex chromatin repression, PSCR). For the human, establishment of MSCI and PSCR have also been reported, but X-linked gene silencing appears to be more variable compared to mouse. To gain more insight into the regulation and significance of MSCI and PSCR among different eutherian species, we have performed a global analysis of XY pairing dynamics, DSB repair, MSCI and PSCR in the domestic dog (Canis lupus familiaris), for which the complete genome sequence has recently become available, allowing a thorough comparative analyses. Results: In addition to PAR synapsis between X and Y, we observed extensive self-synapsis of part of the dog X chromosome, and rapid loss of known markers of DSB repair from that part of the X. Sequencing of RNA from purified spermatocytes and spermatids revealed establishment of MSCI. However, the self-synapsing region of the X displayed higher X-linked gene expression compared to the unsynapsed area in spermatocytes, and was post-meiotically reactivated in spermatids. In contrast, genes in the PAR, which are expected to escape MSCI, were expressed at very low levels in both spermatocytes and spermatids. Our comparative analysis was then used to identify two X-linked genes that may escape MSCI in spermatocytes, and 21 that are specifically re-activated in spermatids of human, mouse ... Article in Journal/Newspaper Canis lupus RePub - Publications from Erasmus University, Rotterdam BMC Genomics 16 1 |
institution |
Open Polar |
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RePub - Publications from Erasmus University, Rotterdam |
op_collection_id |
ftunivrotterdam |
language |
English |
topic |
DNA double-strand break repair Dog Meiosis Meiotic sex chromosome inactivation MSCI Sex chromosomes Spermatids Spermatocytes Spermatogenesis Synaptonemal complex Transcriptome |
spellingShingle |
DNA double-strand break repair Dog Meiosis Meiotic sex chromosome inactivation MSCI Sex chromosomes Spermatids Spermatocytes Spermatogenesis Synaptonemal complex Transcriptome Federici, F. (Federica) Mulugeta, E. (Eskeatnaf) Schoenmakers, S. (Sam) Wassenaar, E. (Evelyne) Hoogerbrugge, J.W. (Jos) Heijden, G.W. (Godfried) van der Cappellen, W.A. (Gert) van Slotman, J.A. (Johan A.) IJcken, W.F.J. (Wilfred) van Laven, J.S.E. (Joop) Grootegoed, J.A. (Anton) Baarends, W.M. (Willy) Incomplete meiotic sex chromosome inactivation in the domestic dog |
topic_facet |
DNA double-strand break repair Dog Meiosis Meiotic sex chromosome inactivation MSCI Sex chromosomes Spermatids Spermatocytes Spermatogenesis Synaptonemal complex Transcriptome |
description |
Background: In mammalian meiotic prophase, homologous chromosome recognition is aided by formation and repair of programmed DNA double-strand breaks (DSBs). Subsequently, stable associations form through homologous chromosome synapsis. In male mouse meiosis, the largely heterologous X and Y chromosomes synapse only in their short pseudoautosomal regions (PARs), and DSBs persist along the unsynapsed non-homologous arms of these sex chromosomes. Asynapsis of these arms and the persistent DSBs then trigger transcriptional silencing through meiotic sex chromosome inactivation (MSCI), resulting in formation of the XY body. This inactive state is partially maintained in post-meiotic haploid spermatids (postmeiotic sex chromatin repression, PSCR). For the human, establishment of MSCI and PSCR have also been reported, but X-linked gene silencing appears to be more variable compared to mouse. To gain more insight into the regulation and significance of MSCI and PSCR among different eutherian species, we have performed a global analysis of XY pairing dynamics, DSB repair, MSCI and PSCR in the domestic dog (Canis lupus familiaris), for which the complete genome sequence has recently become available, allowing a thorough comparative analyses. Results: In addition to PAR synapsis between X and Y, we observed extensive self-synapsis of part of the dog X chromosome, and rapid loss of known markers of DSB repair from that part of the X. Sequencing of RNA from purified spermatocytes and spermatids revealed establishment of MSCI. However, the self-synapsing region of the X displayed higher X-linked gene expression compared to the unsynapsed area in spermatocytes, and was post-meiotically reactivated in spermatids. In contrast, genes in the PAR, which are expected to escape MSCI, were expressed at very low levels in both spermatocytes and spermatids. Our comparative analysis was then used to identify two X-linked genes that may escape MSCI in spermatocytes, and 21 that are specifically re-activated in spermatids of human, mouse ... |
format |
Article in Journal/Newspaper |
author |
Federici, F. (Federica) Mulugeta, E. (Eskeatnaf) Schoenmakers, S. (Sam) Wassenaar, E. (Evelyne) Hoogerbrugge, J.W. (Jos) Heijden, G.W. (Godfried) van der Cappellen, W.A. (Gert) van Slotman, J.A. (Johan A.) IJcken, W.F.J. (Wilfred) van Laven, J.S.E. (Joop) Grootegoed, J.A. (Anton) Baarends, W.M. (Willy) |
author_facet |
Federici, F. (Federica) Mulugeta, E. (Eskeatnaf) Schoenmakers, S. (Sam) Wassenaar, E. (Evelyne) Hoogerbrugge, J.W. (Jos) Heijden, G.W. (Godfried) van der Cappellen, W.A. (Gert) van Slotman, J.A. (Johan A.) IJcken, W.F.J. (Wilfred) van Laven, J.S.E. (Joop) Grootegoed, J.A. (Anton) Baarends, W.M. (Willy) |
author_sort |
Federici, F. (Federica) |
title |
Incomplete meiotic sex chromosome inactivation in the domestic dog |
title_short |
Incomplete meiotic sex chromosome inactivation in the domestic dog |
title_full |
Incomplete meiotic sex chromosome inactivation in the domestic dog |
title_fullStr |
Incomplete meiotic sex chromosome inactivation in the domestic dog |
title_full_unstemmed |
Incomplete meiotic sex chromosome inactivation in the domestic dog |
title_sort |
incomplete meiotic sex chromosome inactivation in the domestic dog |
publishDate |
2015 |
url |
http://repub.eur.nl/pub/90020 https://doi.org/10.1186/s12864-015-1501-9 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
BMC Genomics vol. 16 no. 1 |
op_relation |
http://repub.eur.nl/pub/90020 doi:10.1186/s12864-015-1501-9 urn:hdl:1765/90020 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1186/s12864-015-1501-9 |
container_title |
BMC Genomics |
container_volume |
16 |
container_issue |
1 |
_version_ |
1771544845845790720 |