Central nervous system disease and genital disease in harbor porpoises (Phocoena phocoena) are associated with different herpesviruses
Herpesvirus infection causes disease of variable severity in many species, including cetaceans. However, little is known about herpesvirus infection in harbor porpoises (Phocoena phocoena), despite being widespread in temperate coastal waters of the Northern Hemisphere. Therefore, we examined harbor...
Published in: | Veterinary Research |
---|---|
Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2016
|
Subjects: | |
Online Access: | https://repub.eur.nl/pub/79831 https://doi.org/10.1186/s13567-016-0310-8 |
id |
ftunivrotterdam:oai:repub.eur.nl:79831 |
---|---|
record_format |
openpolar |
spelling |
ftunivrotterdam:oai:repub.eur.nl:79831 2024-06-02T08:13:14+00:00 Central nervous system disease and genital disease in harbor porpoises (Phocoena phocoena) are associated with different herpesviruses Elk, C.E. (Niels) van Bildt, M.W.G. (Marco) van de Run, P.R.W.A. (Peter) van De Jong, A. (Anton) Getu, S. (Sarah) Verjans, G.M.G.M. (George) Osterhaus, A.D.M.E. (Albert) Kuiken, T. (Thijs) 2016-02-09 application/pdf https://repub.eur.nl/pub/79831 https://doi.org/10.1186/s13567-016-0310-8 en eng https://repub.eur.nl/pub/79831 doi:10.1186/s13567-016-0310-8 urn:hdl:1765/79831 Veterinary Research vol. 47 no. 1 Herpesvirus polymerase chain reaction and sequencing Phylogenetic analysis Next-generation sequencing and detection of herpesvirus sequences Sanger sequencing Papillomavirus PCR Virus culture info:eu-repo/semantics/article 2016 ftunivrotterdam https://doi.org/10.1186/s13567-016-0310-8 2024-05-06T16:49:39Z Herpesvirus infection causes disease of variable severity in many species, including cetaceans. However, little is known about herpesvirus infection in harbor porpoises (Phocoena phocoena), despite being widespread in temperate coastal waters of the Northern Hemisphere. Therefore, we examined harbor porpoises that stranded alive in the Netherlands, Belgium, and Germany between 2000 and 2014 for herpesvirus infection and associated disease. Porpoises that died or had to be euthanized were autopsied, and samples were collected for virological and pathological analyses. We found one known herpesvirus (Phocoena phocoena herpesvirus type 1, PPHV-1) - a gammaherpesvirus - and two novel herpesviruses (PPHV-2 and PPHV-3) - both alphaherpesviruses - in these porpoises. A genital plaque, in which PPHV-1 was detected, occurred in 1% (1/117) of porpoises. The plaque was characterized by epithelial hyperplasia and intranuclear inclusion bodies that contained herpesvirus-like particles, and that stained positive by a PPHV-1-specific in situ hybridization test. PPHV-2 occurred in the brain of 2% (1/74) of porpoises. This infection was associated with lymphocytic encephalitis, characterized by neuronal necrosis and intranuclear inclusion bodies containing herpesvirus-like particles. PPHV-3 had a prevalence of 5% (4/74) in brain tissue, 5% (2/43) in blowhole swabs, and 2% (1/43) in genital swabs, but was not associated with disease. Phylogenetically, PPHV-1 was identical to a previously reported herpesvirus from a harbor porpoise, PPHV-2 showed closest identity with two herpesviruses from dolphins, and PPHV-3 showed closest identity with a cervid herpesvirus. In conclusion, harbor porpoises may be infected with at least three different herpesviruses, one of which can cause clinically severe neurological disease. Article in Journal/Newspaper Phocoena phocoena RePub - Publications from Erasmus University, Rotterdam Veterinary Research 47 1 |
institution |
Open Polar |
collection |
RePub - Publications from Erasmus University, Rotterdam |
op_collection_id |
ftunivrotterdam |
language |
English |
topic |
Herpesvirus polymerase chain reaction and sequencing Phylogenetic analysis Next-generation sequencing and detection of herpesvirus sequences Sanger sequencing Papillomavirus PCR Virus culture |
spellingShingle |
Herpesvirus polymerase chain reaction and sequencing Phylogenetic analysis Next-generation sequencing and detection of herpesvirus sequences Sanger sequencing Papillomavirus PCR Virus culture Elk, C.E. (Niels) van Bildt, M.W.G. (Marco) van de Run, P.R.W.A. (Peter) van De Jong, A. (Anton) Getu, S. (Sarah) Verjans, G.M.G.M. (George) Osterhaus, A.D.M.E. (Albert) Kuiken, T. (Thijs) Central nervous system disease and genital disease in harbor porpoises (Phocoena phocoena) are associated with different herpesviruses |
topic_facet |
Herpesvirus polymerase chain reaction and sequencing Phylogenetic analysis Next-generation sequencing and detection of herpesvirus sequences Sanger sequencing Papillomavirus PCR Virus culture |
description |
Herpesvirus infection causes disease of variable severity in many species, including cetaceans. However, little is known about herpesvirus infection in harbor porpoises (Phocoena phocoena), despite being widespread in temperate coastal waters of the Northern Hemisphere. Therefore, we examined harbor porpoises that stranded alive in the Netherlands, Belgium, and Germany between 2000 and 2014 for herpesvirus infection and associated disease. Porpoises that died or had to be euthanized were autopsied, and samples were collected for virological and pathological analyses. We found one known herpesvirus (Phocoena phocoena herpesvirus type 1, PPHV-1) - a gammaherpesvirus - and two novel herpesviruses (PPHV-2 and PPHV-3) - both alphaherpesviruses - in these porpoises. A genital plaque, in which PPHV-1 was detected, occurred in 1% (1/117) of porpoises. The plaque was characterized by epithelial hyperplasia and intranuclear inclusion bodies that contained herpesvirus-like particles, and that stained positive by a PPHV-1-specific in situ hybridization test. PPHV-2 occurred in the brain of 2% (1/74) of porpoises. This infection was associated with lymphocytic encephalitis, characterized by neuronal necrosis and intranuclear inclusion bodies containing herpesvirus-like particles. PPHV-3 had a prevalence of 5% (4/74) in brain tissue, 5% (2/43) in blowhole swabs, and 2% (1/43) in genital swabs, but was not associated with disease. Phylogenetically, PPHV-1 was identical to a previously reported herpesvirus from a harbor porpoise, PPHV-2 showed closest identity with two herpesviruses from dolphins, and PPHV-3 showed closest identity with a cervid herpesvirus. In conclusion, harbor porpoises may be infected with at least three different herpesviruses, one of which can cause clinically severe neurological disease. |
format |
Article in Journal/Newspaper |
author |
Elk, C.E. (Niels) van Bildt, M.W.G. (Marco) van de Run, P.R.W.A. (Peter) van De Jong, A. (Anton) Getu, S. (Sarah) Verjans, G.M.G.M. (George) Osterhaus, A.D.M.E. (Albert) Kuiken, T. (Thijs) |
author_facet |
Elk, C.E. (Niels) van Bildt, M.W.G. (Marco) van de Run, P.R.W.A. (Peter) van De Jong, A. (Anton) Getu, S. (Sarah) Verjans, G.M.G.M. (George) Osterhaus, A.D.M.E. (Albert) Kuiken, T. (Thijs) |
author_sort |
Elk, C.E. (Niels) van |
title |
Central nervous system disease and genital disease in harbor porpoises (Phocoena phocoena) are associated with different herpesviruses |
title_short |
Central nervous system disease and genital disease in harbor porpoises (Phocoena phocoena) are associated with different herpesviruses |
title_full |
Central nervous system disease and genital disease in harbor porpoises (Phocoena phocoena) are associated with different herpesviruses |
title_fullStr |
Central nervous system disease and genital disease in harbor porpoises (Phocoena phocoena) are associated with different herpesviruses |
title_full_unstemmed |
Central nervous system disease and genital disease in harbor porpoises (Phocoena phocoena) are associated with different herpesviruses |
title_sort |
central nervous system disease and genital disease in harbor porpoises (phocoena phocoena) are associated with different herpesviruses |
publishDate |
2016 |
url |
https://repub.eur.nl/pub/79831 https://doi.org/10.1186/s13567-016-0310-8 |
genre |
Phocoena phocoena |
genre_facet |
Phocoena phocoena |
op_source |
Veterinary Research vol. 47 no. 1 |
op_relation |
https://repub.eur.nl/pub/79831 doi:10.1186/s13567-016-0310-8 urn:hdl:1765/79831 |
op_doi |
https://doi.org/10.1186/s13567-016-0310-8 |
container_title |
Veterinary Research |
container_volume |
47 |
container_issue |
1 |
_version_ |
1800736668877062144 |