Immune functions in beluga whales (Delphinapterus leucas): Evaluation of natural killer cell act
Natural killer (NK) activity, an important non-specific defense mechanism against viral infections and tumors, was demonstrated in beluga whales using two different methods: 51Cr release and flow cytometry. Using the 51Cr release assay, NK activity in belugas was shown to be higher against K-562 tha...
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ftunivrotterdam:oai:repub.eur.nl:3624 2023-07-16T03:57:43+02:00 Immune functions in beluga whales (Delphinapterus leucas): Evaluation of natural killer cell act De Guise, S. (Sylvain) Ross, P.S. (Peter) Osterhaus, A.D.M.E. (Albert) Martineau, D. (Daniel) Beland, P. Fournier, M. (Michel) 1997-09-19 application/pdf http://repub.eur.nl/pub/3624 https://doi.org/10.1016/S0165-2427(97)00035-4 en eng http://repub.eur.nl/pub/3624 doi:10.1016/S0165-2427(97)00035-4 urn:hdl:1765/3624 info:eu-repo/semantics/openAccess Veterinary Immunology and Immunopathology vol. 58 no. 3-4, pp. 345-354 Animals Cytotoxicity Immunologic Killer Cells Natural/*immunology Support Non-U.S. Gov't U.S. Gov't Non-P.H.S Whales/*immunology info:eu-repo/semantics/article 1997 ftunivrotterdam https://doi.org/10.1016/S0165-2427(97)00035-4 2023-06-26T22:14:43Z Natural killer (NK) activity, an important non-specific defense mechanism against viral infections and tumors, was demonstrated in beluga whales using two different methods: 51Cr release and flow cytometry. Using the 51Cr release assay, NK activity in belugas was shown to be higher against K-562 than against YAC-1 cell lines. Moreover, it was enhanced by the addition of human recombinant interleukin-2 with both cell lines. NK activity evaluated by flow cytometry in the peripheral blood of eight belugas increased when the effector:target cell (E:T) ratio increased, and averaged 13.9% +/- 3.8% (range 9.9% to 17.8%) at an E:T ratio of 100:1. While NK activity could be readily detected using both methods, the lack of radio-isotopes and related laboratory room make the flow cytometric method a viable and safe alternative. The evaluation of this function in cetaceans could lead to a better understanding of the early events that lead to viral epizootics in populations of marine mammals in different parts of the world, as well as to the high prevalence of neoplasms in St. Lawrence beluga whales. Article in Journal/Newspaper Beluga Beluga* Delphinapterus leucas RePub - Publications from Erasmus University, Rotterdam Veterinary Immunology and Immunopathology 58 3-4 345 354 |
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RePub - Publications from Erasmus University, Rotterdam |
op_collection_id |
ftunivrotterdam |
language |
English |
topic |
Animals Cytotoxicity Immunologic Killer Cells Natural/*immunology Support Non-U.S. Gov't U.S. Gov't Non-P.H.S Whales/*immunology |
spellingShingle |
Animals Cytotoxicity Immunologic Killer Cells Natural/*immunology Support Non-U.S. Gov't U.S. Gov't Non-P.H.S Whales/*immunology De Guise, S. (Sylvain) Ross, P.S. (Peter) Osterhaus, A.D.M.E. (Albert) Martineau, D. (Daniel) Beland, P. Fournier, M. (Michel) Immune functions in beluga whales (Delphinapterus leucas): Evaluation of natural killer cell act |
topic_facet |
Animals Cytotoxicity Immunologic Killer Cells Natural/*immunology Support Non-U.S. Gov't U.S. Gov't Non-P.H.S Whales/*immunology |
description |
Natural killer (NK) activity, an important non-specific defense mechanism against viral infections and tumors, was demonstrated in beluga whales using two different methods: 51Cr release and flow cytometry. Using the 51Cr release assay, NK activity in belugas was shown to be higher against K-562 than against YAC-1 cell lines. Moreover, it was enhanced by the addition of human recombinant interleukin-2 with both cell lines. NK activity evaluated by flow cytometry in the peripheral blood of eight belugas increased when the effector:target cell (E:T) ratio increased, and averaged 13.9% +/- 3.8% (range 9.9% to 17.8%) at an E:T ratio of 100:1. While NK activity could be readily detected using both methods, the lack of radio-isotopes and related laboratory room make the flow cytometric method a viable and safe alternative. The evaluation of this function in cetaceans could lead to a better understanding of the early events that lead to viral epizootics in populations of marine mammals in different parts of the world, as well as to the high prevalence of neoplasms in St. Lawrence beluga whales. |
format |
Article in Journal/Newspaper |
author |
De Guise, S. (Sylvain) Ross, P.S. (Peter) Osterhaus, A.D.M.E. (Albert) Martineau, D. (Daniel) Beland, P. Fournier, M. (Michel) |
author_facet |
De Guise, S. (Sylvain) Ross, P.S. (Peter) Osterhaus, A.D.M.E. (Albert) Martineau, D. (Daniel) Beland, P. Fournier, M. (Michel) |
author_sort |
De Guise, S. (Sylvain) |
title |
Immune functions in beluga whales (Delphinapterus leucas): Evaluation of natural killer cell act |
title_short |
Immune functions in beluga whales (Delphinapterus leucas): Evaluation of natural killer cell act |
title_full |
Immune functions in beluga whales (Delphinapterus leucas): Evaluation of natural killer cell act |
title_fullStr |
Immune functions in beluga whales (Delphinapterus leucas): Evaluation of natural killer cell act |
title_full_unstemmed |
Immune functions in beluga whales (Delphinapterus leucas): Evaluation of natural killer cell act |
title_sort |
immune functions in beluga whales (delphinapterus leucas): evaluation of natural killer cell act |
publishDate |
1997 |
url |
http://repub.eur.nl/pub/3624 https://doi.org/10.1016/S0165-2427(97)00035-4 |
genre |
Beluga Beluga* Delphinapterus leucas |
genre_facet |
Beluga Beluga* Delphinapterus leucas |
op_source |
Veterinary Immunology and Immunopathology vol. 58 no. 3-4, pp. 345-354 |
op_relation |
http://repub.eur.nl/pub/3624 doi:10.1016/S0165-2427(97)00035-4 urn:hdl:1765/3624 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/S0165-2427(97)00035-4 |
container_title |
Veterinary Immunology and Immunopathology |
container_volume |
58 |
container_issue |
3-4 |
container_start_page |
345 |
op_container_end_page |
354 |
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1771544419394125824 |