Vaccination of harbour seals (Phoca vitulina) against phocid distemper with two different inactivated canine distemper virus (CDV) vaccines.
Two inactivated canine distemper virus (CDV) vaccines--an adjuvanted whole inactivated virus and a subunit ISCOM preparation--were tested for their ability to induce protective immunity in harbour seals (Phoca vitulina) against phocid distemper, a disease that recently killed greater than 17,000 har...
Published in: | Vaccine |
---|---|
Main Authors: | , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
1989
|
Subjects: | |
Online Access: | http://repub.eur.nl/pub/3379 https://doi.org/10.1016/0264-410X(89)90276-4 |
id |
ftunivrotterdam:oai:repub.eur.nl:3379 |
---|---|
record_format |
openpolar |
spelling |
ftunivrotterdam:oai:repub.eur.nl:3379 2023-07-16T04:00:30+02:00 Vaccination of harbour seals (Phoca vitulina) against phocid distemper with two different inactivated canine distemper virus (CDV) vaccines. Visser, I.K.G. (Ilona) Bildt, M.W.G. (Marco) van de Brugge, H.N. Reijnders, P.J.H. Vedder, E.J. (Lies) Kuiper, J. Vries, P. (Petra) de Groen, J. (Jan) Walvoort, H.C. Uytdehaag, F.G.C.M. (Fons) Osterhaus, A.D.M.E. (Albert) 1989-01-01 application/pdf http://repub.eur.nl/pub/3379 https://doi.org/10.1016/0264-410X(89)90276-4 en eng http://repub.eur.nl/pub/3379 doi:10.1016/0264-410X(89)90276-4 urn:hdl:1765/3379 info:eu-repo/semantics/openAccess Vaccine, pp. 521-526 info:eu-repo/semantics/article 1989 ftunivrotterdam https://doi.org/10.1016/0264-410X(89)90276-4 2023-06-26T22:14:49Z Two inactivated canine distemper virus (CDV) vaccines--an adjuvanted whole inactivated virus and a subunit ISCOM preparation--were tested for their ability to induce protective immunity in harbour seals (Phoca vitulina) against phocid distemper, a disease that recently killed greater than 17,000 harbour seals in the North and Baltic seas, and was shown to be caused by infection with a newly discovered morbillivirus, which is antigenically closely related to CDV. Four CDV seronegative harbour seals were vaccinated three times with the whole-virus vaccine, two with the ISCOM subunit vaccine and two were sham-vaccinated with an antigen-free preparation. Ten days after the last vaccination, when all six vaccinated animals had developed CDV neutralizing antibody titres ranging from 300 to 3000, all eight animals were challenged by the oculonasal and the peritoneal routes, with an organ suspension from dead seals. None of the six vaccinated animals developed clinical signs. The two sham-vaccinated seals died on days 14 and 18, respectively, after having shown a body temperature rise, respiratory symptoms and weight loss. In organs from both dead animals morbillivirus antigen was demonstrated with an enzyme-linked immunosorbent assay and an immunofluorescence assay. One of these two animals had developed a low titre of CDV-specific antibodies just before death. These data clearly indicate that seals can be protected from fatal challenge with the phocid distemper virus (PDV), by vaccination with certain inactivated CDV vaccines. They also reconfirm that infection with PDV should be considered the primary cause of the recent epizootic in seals. Article in Journal/Newspaper Phoca vitulina RePub - Publications from Erasmus University, Rotterdam Vaccine 7 6 521 526 |
institution |
Open Polar |
collection |
RePub - Publications from Erasmus University, Rotterdam |
op_collection_id |
ftunivrotterdam |
language |
English |
description |
Two inactivated canine distemper virus (CDV) vaccines--an adjuvanted whole inactivated virus and a subunit ISCOM preparation--were tested for their ability to induce protective immunity in harbour seals (Phoca vitulina) against phocid distemper, a disease that recently killed greater than 17,000 harbour seals in the North and Baltic seas, and was shown to be caused by infection with a newly discovered morbillivirus, which is antigenically closely related to CDV. Four CDV seronegative harbour seals were vaccinated three times with the whole-virus vaccine, two with the ISCOM subunit vaccine and two were sham-vaccinated with an antigen-free preparation. Ten days after the last vaccination, when all six vaccinated animals had developed CDV neutralizing antibody titres ranging from 300 to 3000, all eight animals were challenged by the oculonasal and the peritoneal routes, with an organ suspension from dead seals. None of the six vaccinated animals developed clinical signs. The two sham-vaccinated seals died on days 14 and 18, respectively, after having shown a body temperature rise, respiratory symptoms and weight loss. In organs from both dead animals morbillivirus antigen was demonstrated with an enzyme-linked immunosorbent assay and an immunofluorescence assay. One of these two animals had developed a low titre of CDV-specific antibodies just before death. These data clearly indicate that seals can be protected from fatal challenge with the phocid distemper virus (PDV), by vaccination with certain inactivated CDV vaccines. They also reconfirm that infection with PDV should be considered the primary cause of the recent epizootic in seals. |
format |
Article in Journal/Newspaper |
author |
Visser, I.K.G. (Ilona) Bildt, M.W.G. (Marco) van de Brugge, H.N. Reijnders, P.J.H. Vedder, E.J. (Lies) Kuiper, J. Vries, P. (Petra) de Groen, J. (Jan) Walvoort, H.C. Uytdehaag, F.G.C.M. (Fons) Osterhaus, A.D.M.E. (Albert) |
spellingShingle |
Visser, I.K.G. (Ilona) Bildt, M.W.G. (Marco) van de Brugge, H.N. Reijnders, P.J.H. Vedder, E.J. (Lies) Kuiper, J. Vries, P. (Petra) de Groen, J. (Jan) Walvoort, H.C. Uytdehaag, F.G.C.M. (Fons) Osterhaus, A.D.M.E. (Albert) Vaccination of harbour seals (Phoca vitulina) against phocid distemper with two different inactivated canine distemper virus (CDV) vaccines. |
author_facet |
Visser, I.K.G. (Ilona) Bildt, M.W.G. (Marco) van de Brugge, H.N. Reijnders, P.J.H. Vedder, E.J. (Lies) Kuiper, J. Vries, P. (Petra) de Groen, J. (Jan) Walvoort, H.C. Uytdehaag, F.G.C.M. (Fons) Osterhaus, A.D.M.E. (Albert) |
author_sort |
Visser, I.K.G. (Ilona) |
title |
Vaccination of harbour seals (Phoca vitulina) against phocid distemper with two different inactivated canine distemper virus (CDV) vaccines. |
title_short |
Vaccination of harbour seals (Phoca vitulina) against phocid distemper with two different inactivated canine distemper virus (CDV) vaccines. |
title_full |
Vaccination of harbour seals (Phoca vitulina) against phocid distemper with two different inactivated canine distemper virus (CDV) vaccines. |
title_fullStr |
Vaccination of harbour seals (Phoca vitulina) against phocid distemper with two different inactivated canine distemper virus (CDV) vaccines. |
title_full_unstemmed |
Vaccination of harbour seals (Phoca vitulina) against phocid distemper with two different inactivated canine distemper virus (CDV) vaccines. |
title_sort |
vaccination of harbour seals (phoca vitulina) against phocid distemper with two different inactivated canine distemper virus (cdv) vaccines. |
publishDate |
1989 |
url |
http://repub.eur.nl/pub/3379 https://doi.org/10.1016/0264-410X(89)90276-4 |
genre |
Phoca vitulina |
genre_facet |
Phoca vitulina |
op_source |
Vaccine, pp. 521-526 |
op_relation |
http://repub.eur.nl/pub/3379 doi:10.1016/0264-410X(89)90276-4 urn:hdl:1765/3379 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/0264-410X(89)90276-4 |
container_title |
Vaccine |
container_volume |
7 |
container_issue |
6 |
container_start_page |
521 |
op_container_end_page |
526 |
_version_ |
1771549356001853440 |