Spectroscopic properties of the nitric oxide derivative of ferrous man, horse, and ruminant hemoglobins: a comparative study
The spectroscopic (EPR and absorbance) properties of the nitric oxide derivative of ferrous man, horse, buffalo, deer, mouflon, musk ox, ox, and reindeer hemoglobin (HbNO) have been investigated in the absence of any allosteric effector at pH 6.5 (in 0.1 M 2-[N-morpholino]ethanesulphonic acid/NaOH c...
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1992
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ftunivromatorver:oai:art.torvergata.it:2108/54783 2023-08-20T04:08:00+02:00 Spectroscopic properties of the nitric oxide derivative of ferrous man, horse, and ruminant hemoglobins: a comparative study Ascenzi, P Petruzzelli, R Bolognesi, M Giardina, B. COLETTA, MASSIMILIANO DESIDERI, ALESSANDRO POLIZIO, FRANCESCA CONDO', SAVERIO GIOVANNI Ascenzi, P Coletta, M Desideri, A Petruzzelli, R Polizio, F Bolognesi, M Condo', Sg Giardina, B 1992-01 http://hdl.handle.net/2108/54783 eng eng volume:45 issue:1 firstpage:31 lastpage:37 journal:JOURNAL OF INORGANIC BIOCHEMISTRY http://hdl.handle.net/2108/54783 Morpholine Animal Models Molecular Human Hydrogen-Ion Concentration Horse Ruminant Reindeer Nitric Oxide Cattle Hemoglobin Alkanesulfonate Electron Spin Resonance Spectroscopy Buffaloe Spectrophotometry Alkanesulfonic Acid Allosteric Regulation Deer Protein Conformation Settore BIO/10 - BIOCHIMICA info:eu-repo/semantics/article 1992 ftunivromatorver 2023-08-01T23:07:37Z The spectroscopic (EPR and absorbance) properties of the nitric oxide derivative of ferrous man, horse, buffalo, deer, mouflon, musk ox, ox, and reindeer hemoglobin (HbNO) have been investigated in the absence of any allosteric effector at pH 6.5 (in 0.1 M 2-[N-morpholino]ethanesulphonic acid/NaOH chloride-free buffer system), as well as at 100 K and/or 20 degrees C. Man and horse HbNO show spectroscopic properties that are generally taken as typical of the high affinity state of ferrous tetrameric Hb's; on the other hand, the spectroscopic properties of ruminant (i.e., buffalo, deer, mouflon, musk ox, ox, and reindeer) HbNO are characteristic of the low affinity conformation. These results are in keeping with the functional properties of the mammalian Hb's considered and have been related to the peculiar low oxygen affinity of ruminant Hb's. Article in Journal/Newspaper musk ox Universitá degli Studi di Roma "Tor Vergata": ART - Archivio Istituzionale della Ricerca |
institution |
Open Polar |
collection |
Universitá degli Studi di Roma "Tor Vergata": ART - Archivio Istituzionale della Ricerca |
op_collection_id |
ftunivromatorver |
language |
English |
topic |
Morpholine Animal Models Molecular Human Hydrogen-Ion Concentration Horse Ruminant Reindeer Nitric Oxide Cattle Hemoglobin Alkanesulfonate Electron Spin Resonance Spectroscopy Buffaloe Spectrophotometry Alkanesulfonic Acid Allosteric Regulation Deer Protein Conformation Settore BIO/10 - BIOCHIMICA |
spellingShingle |
Morpholine Animal Models Molecular Human Hydrogen-Ion Concentration Horse Ruminant Reindeer Nitric Oxide Cattle Hemoglobin Alkanesulfonate Electron Spin Resonance Spectroscopy Buffaloe Spectrophotometry Alkanesulfonic Acid Allosteric Regulation Deer Protein Conformation Settore BIO/10 - BIOCHIMICA Ascenzi, P Petruzzelli, R Bolognesi, M Giardina, B. COLETTA, MASSIMILIANO DESIDERI, ALESSANDRO POLIZIO, FRANCESCA CONDO', SAVERIO GIOVANNI Spectroscopic properties of the nitric oxide derivative of ferrous man, horse, and ruminant hemoglobins: a comparative study |
topic_facet |
Morpholine Animal Models Molecular Human Hydrogen-Ion Concentration Horse Ruminant Reindeer Nitric Oxide Cattle Hemoglobin Alkanesulfonate Electron Spin Resonance Spectroscopy Buffaloe Spectrophotometry Alkanesulfonic Acid Allosteric Regulation Deer Protein Conformation Settore BIO/10 - BIOCHIMICA |
description |
The spectroscopic (EPR and absorbance) properties of the nitric oxide derivative of ferrous man, horse, buffalo, deer, mouflon, musk ox, ox, and reindeer hemoglobin (HbNO) have been investigated in the absence of any allosteric effector at pH 6.5 (in 0.1 M 2-[N-morpholino]ethanesulphonic acid/NaOH chloride-free buffer system), as well as at 100 K and/or 20 degrees C. Man and horse HbNO show spectroscopic properties that are generally taken as typical of the high affinity state of ferrous tetrameric Hb's; on the other hand, the spectroscopic properties of ruminant (i.e., buffalo, deer, mouflon, musk ox, ox, and reindeer) HbNO are characteristic of the low affinity conformation. These results are in keeping with the functional properties of the mammalian Hb's considered and have been related to the peculiar low oxygen affinity of ruminant Hb's. |
author2 |
Ascenzi, P Coletta, M Desideri, A Petruzzelli, R Polizio, F Bolognesi, M Condo', Sg Giardina, B |
format |
Article in Journal/Newspaper |
author |
Ascenzi, P Petruzzelli, R Bolognesi, M Giardina, B. COLETTA, MASSIMILIANO DESIDERI, ALESSANDRO POLIZIO, FRANCESCA CONDO', SAVERIO GIOVANNI |
author_facet |
Ascenzi, P Petruzzelli, R Bolognesi, M Giardina, B. COLETTA, MASSIMILIANO DESIDERI, ALESSANDRO POLIZIO, FRANCESCA CONDO', SAVERIO GIOVANNI |
author_sort |
Ascenzi, P |
title |
Spectroscopic properties of the nitric oxide derivative of ferrous man, horse, and ruminant hemoglobins: a comparative study |
title_short |
Spectroscopic properties of the nitric oxide derivative of ferrous man, horse, and ruminant hemoglobins: a comparative study |
title_full |
Spectroscopic properties of the nitric oxide derivative of ferrous man, horse, and ruminant hemoglobins: a comparative study |
title_fullStr |
Spectroscopic properties of the nitric oxide derivative of ferrous man, horse, and ruminant hemoglobins: a comparative study |
title_full_unstemmed |
Spectroscopic properties of the nitric oxide derivative of ferrous man, horse, and ruminant hemoglobins: a comparative study |
title_sort |
spectroscopic properties of the nitric oxide derivative of ferrous man, horse, and ruminant hemoglobins: a comparative study |
publishDate |
1992 |
url |
http://hdl.handle.net/2108/54783 |
genre |
musk ox |
genre_facet |
musk ox |
op_relation |
volume:45 issue:1 firstpage:31 lastpage:37 journal:JOURNAL OF INORGANIC BIOCHEMISTRY http://hdl.handle.net/2108/54783 |
_version_ |
1774720017243308032 |