Sponges as natural environmental DNA samplers
At a time of unprecedented impacts on marine biodiversity, scientists are rapidly becoming persuaded by the potential of screening large swathes of the oceans through the retrieval, amplification and sequencing of trace DNA fragments left behind by marine organisms; an approach known as 'enviro...
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Online Access: | https://hdl.handle.net/2108/311741 https://doi.org/10.1016/j.cub.2019.04.031 |
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ftunivromatorver:oai:art.torvergata.it:2108/311741 2024-02-27T08:35:14+00:00 Sponges as natural environmental DNA samplers Mariani, Stefano Baillie, Charles Colosimo, Giuliano Riesgo, Ana Mariani, S Baillie, C Colosimo, G Riesgo, A 2019-06-03 https://hdl.handle.net/2108/311741 https://doi.org/10.1016/j.cub.2019.04.031 eng eng CELL PRESS info:eu-repo/semantics/altIdentifier/pmid/31163139 info:eu-repo/semantics/altIdentifier/wos/WOS:000470902000003 volume:29 issue:11 firstpage:401 lastpage:402 numberofpages:2 journal:CURRENT BIOLOGY https://hdl.handle.net/2108/311741 doi:10.1016/j.cub.2019.04.031 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85066273485 Settore BIO/05 - ZOOLOGIA info:eu-repo/semantics/article 2019 ftunivromatorver https://doi.org/10.1016/j.cub.2019.04.031 2024-01-31T00:01:34Z At a time of unprecedented impacts on marine biodiversity, scientists are rapidly becoming persuaded by the potential of screening large swathes of the oceans through the retrieval, amplification and sequencing of trace DNA fragments left behind by marine organisms; an approach known as 'environmental DNA' (eDNA) [1]. In trying to circumvent the many challenges associated with water filtration and DNA isolation from environmental samples, significant investment is being made in high-tech solutions, such as automated underwater vehicles and robots [2]. Here, instead, we explored a simpler, alternative option, based on the recovery of eDNA from sponges (phylum Porifera), the planet's most effective water-filterers. We obtained sponge samples from Mediterranean and Antarctic surveys, extracted total DNA from their tissues, and obtained tens of thousands of fish DNA reads via metabarcoding, which were able to clearly distinguish samples from the two regions. One Antarctic sample yielded hundreds of reads from chinstrap penguin (Pygoscelis antarcticus) and Weddell seal (Leptonychotes weddellii). We argue that this 'natural sampler DNA' (nsDNA) approach is poised to become a powerful, affordable, universal tool for aquatic biodiversity monitoring globally. Article in Journal/Newspaper Antarc* Antarctic antarcticus Chinstrap penguin Weddell Seal Universitá degli Studi di Roma "Tor Vergata": ART - Archivio Istituzionale della Ricerca Antarctic Weddell Current Biology 29 11 R401 R402 |
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Open Polar |
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Universitá degli Studi di Roma "Tor Vergata": ART - Archivio Istituzionale della Ricerca |
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ftunivromatorver |
language |
English |
topic |
Settore BIO/05 - ZOOLOGIA |
spellingShingle |
Settore BIO/05 - ZOOLOGIA Mariani, Stefano Baillie, Charles Colosimo, Giuliano Riesgo, Ana Sponges as natural environmental DNA samplers |
topic_facet |
Settore BIO/05 - ZOOLOGIA |
description |
At a time of unprecedented impacts on marine biodiversity, scientists are rapidly becoming persuaded by the potential of screening large swathes of the oceans through the retrieval, amplification and sequencing of trace DNA fragments left behind by marine organisms; an approach known as 'environmental DNA' (eDNA) [1]. In trying to circumvent the many challenges associated with water filtration and DNA isolation from environmental samples, significant investment is being made in high-tech solutions, such as automated underwater vehicles and robots [2]. Here, instead, we explored a simpler, alternative option, based on the recovery of eDNA from sponges (phylum Porifera), the planet's most effective water-filterers. We obtained sponge samples from Mediterranean and Antarctic surveys, extracted total DNA from their tissues, and obtained tens of thousands of fish DNA reads via metabarcoding, which were able to clearly distinguish samples from the two regions. One Antarctic sample yielded hundreds of reads from chinstrap penguin (Pygoscelis antarcticus) and Weddell seal (Leptonychotes weddellii). We argue that this 'natural sampler DNA' (nsDNA) approach is poised to become a powerful, affordable, universal tool for aquatic biodiversity monitoring globally. |
author2 |
Mariani, S Baillie, C Colosimo, G Riesgo, A |
format |
Article in Journal/Newspaper |
author |
Mariani, Stefano Baillie, Charles Colosimo, Giuliano Riesgo, Ana |
author_facet |
Mariani, Stefano Baillie, Charles Colosimo, Giuliano Riesgo, Ana |
author_sort |
Mariani, Stefano |
title |
Sponges as natural environmental DNA samplers |
title_short |
Sponges as natural environmental DNA samplers |
title_full |
Sponges as natural environmental DNA samplers |
title_fullStr |
Sponges as natural environmental DNA samplers |
title_full_unstemmed |
Sponges as natural environmental DNA samplers |
title_sort |
sponges as natural environmental dna samplers |
publisher |
CELL PRESS |
publishDate |
2019 |
url |
https://hdl.handle.net/2108/311741 https://doi.org/10.1016/j.cub.2019.04.031 |
geographic |
Antarctic Weddell |
geographic_facet |
Antarctic Weddell |
genre |
Antarc* Antarctic antarcticus Chinstrap penguin Weddell Seal |
genre_facet |
Antarc* Antarctic antarcticus Chinstrap penguin Weddell Seal |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/31163139 info:eu-repo/semantics/altIdentifier/wos/WOS:000470902000003 volume:29 issue:11 firstpage:401 lastpage:402 numberofpages:2 journal:CURRENT BIOLOGY https://hdl.handle.net/2108/311741 doi:10.1016/j.cub.2019.04.031 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85066273485 |
op_doi |
https://doi.org/10.1016/j.cub.2019.04.031 |
container_title |
Current Biology |
container_volume |
29 |
container_issue |
11 |
container_start_page |
R401 |
op_container_end_page |
R402 |
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1792041699472572416 |