Damage escape and repair in dried Chroococcidiopsis spp. from hot and cold deserts exposed to simulated space and Martian conditions
The cyanobacterium Chroococcidiopsis, overlain by 3mm of Antarctic sandstone, was exposed as dried multilayers to simulated space and martian conditions. Ground-based experiments were conducted in the context of Lichens and Fungi Experiments (EXPOSE-E mission, European Space Agency), which were perf...
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Mary Ann Liebert Incorporated
2011
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Online Access: | http://hdl.handle.net/2108/13681 https://doi.org/10.1089/ast.2009.0430 |
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ftunivromatorver:oai:art.torvergata.it:2108/13681 2024-02-27T08:33:27+00:00 Damage escape and repair in dried Chroococcidiopsis spp. from hot and cold deserts exposed to simulated space and Martian conditions BILLI, DANIELA Viaggiu, E Cockell CS Rabbow, E Horneck, G Onofri, S. Billi, D Viaggiu, E Cockell, C Rabbow, E Horneck, G Onofri, S 2011 http://hdl.handle.net/2108/13681 https://doi.org/10.1089/ast.2009.0430 eng eng Mary Ann Liebert Incorporated country:US info:eu-repo/semantics/altIdentifier/pmid/21294638 info:eu-repo/semantics/altIdentifier/wos/WOS:000287689200007 volume:11 issue:1 firstpage:65 lastpage:73 numberofpages:9 journal:ASTROBIOLOGY http://hdl.handle.net/2108/13681 doi:10.1089/ast.2009.0430 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-79952019471 info:eu-repo/semantics/restrictedAccess Astrobiology Endolithic cyanobacteri Chroococcidiopsi Ground-based experiments Settore BIO/01 - BOTANICA GENERALE info:eu-repo/semantics/article 2011 ftunivromatorver https://doi.org/10.1089/ast.2009.0430 2024-01-31T00:04:15Z The cyanobacterium Chroococcidiopsis, overlain by 3mm of Antarctic sandstone, was exposed as dried multilayers to simulated space and martian conditions. Ground-based experiments were conducted in the context of Lichens and Fungi Experiments (EXPOSE-E mission, European Space Agency), which were performed to evaluate, after 1.5 years on the International Space Station, the survival of cyanobacteria (Chroococcidiopsis), lichens, and fungi colonized on Antarctic rock. The survival potential and the role played by protection and repair mechanisms in the response of dried Chroococcidiopsis cells to ground-based experiments were both investigated. Different methods were employed, including evaluation of the colony-forming ability, single-cell analysis of subcellular integrities based on membrane integrity molecular and redox probes, evaluation of the photosynthetic pigment autofluorescence, and assessment of the genomic DNA integrity with a PCR-based assay. Desiccation survivors of strain CCMEE 123 (coastal desert, Chile) were better suited than CCMEE 134 (Beacon Valley, Antarctica) to withstand cellular damage imposed by simulated space and martian conditions. Exposed dried cells of strain CCMEE 123 formed colonies, maintained subcellular integrities, and, depending on the exposure conditions, also escaped DNA damage or repaired the induced damage upon rewetting. Article in Journal/Newspaper Antarc* Antarctic Antarctica Universitá degli Studi di Roma "Tor Vergata": ART - Archivio Istituzionale della Ricerca Antarctic Beacon Valley ENVELOPE(160.650,160.650,-77.817,-77.817) Astrobiology 11 1 65 73 |
institution |
Open Polar |
collection |
Universitá degli Studi di Roma "Tor Vergata": ART - Archivio Istituzionale della Ricerca |
op_collection_id |
ftunivromatorver |
language |
English |
topic |
Astrobiology Endolithic cyanobacteri Chroococcidiopsi Ground-based experiments Settore BIO/01 - BOTANICA GENERALE |
spellingShingle |
Astrobiology Endolithic cyanobacteri Chroococcidiopsi Ground-based experiments Settore BIO/01 - BOTANICA GENERALE BILLI, DANIELA Viaggiu, E Cockell CS Rabbow, E Horneck, G Onofri, S. Damage escape and repair in dried Chroococcidiopsis spp. from hot and cold deserts exposed to simulated space and Martian conditions |
topic_facet |
Astrobiology Endolithic cyanobacteri Chroococcidiopsi Ground-based experiments Settore BIO/01 - BOTANICA GENERALE |
description |
The cyanobacterium Chroococcidiopsis, overlain by 3mm of Antarctic sandstone, was exposed as dried multilayers to simulated space and martian conditions. Ground-based experiments were conducted in the context of Lichens and Fungi Experiments (EXPOSE-E mission, European Space Agency), which were performed to evaluate, after 1.5 years on the International Space Station, the survival of cyanobacteria (Chroococcidiopsis), lichens, and fungi colonized on Antarctic rock. The survival potential and the role played by protection and repair mechanisms in the response of dried Chroococcidiopsis cells to ground-based experiments were both investigated. Different methods were employed, including evaluation of the colony-forming ability, single-cell analysis of subcellular integrities based on membrane integrity molecular and redox probes, evaluation of the photosynthetic pigment autofluorescence, and assessment of the genomic DNA integrity with a PCR-based assay. Desiccation survivors of strain CCMEE 123 (coastal desert, Chile) were better suited than CCMEE 134 (Beacon Valley, Antarctica) to withstand cellular damage imposed by simulated space and martian conditions. Exposed dried cells of strain CCMEE 123 formed colonies, maintained subcellular integrities, and, depending on the exposure conditions, also escaped DNA damage or repaired the induced damage upon rewetting. |
author2 |
Billi, D Viaggiu, E Cockell, C Rabbow, E Horneck, G Onofri, S |
format |
Article in Journal/Newspaper |
author |
BILLI, DANIELA Viaggiu, E Cockell CS Rabbow, E Horneck, G Onofri, S. |
author_facet |
BILLI, DANIELA Viaggiu, E Cockell CS Rabbow, E Horneck, G Onofri, S. |
author_sort |
BILLI, DANIELA |
title |
Damage escape and repair in dried Chroococcidiopsis spp. from hot and cold deserts exposed to simulated space and Martian conditions |
title_short |
Damage escape and repair in dried Chroococcidiopsis spp. from hot and cold deserts exposed to simulated space and Martian conditions |
title_full |
Damage escape and repair in dried Chroococcidiopsis spp. from hot and cold deserts exposed to simulated space and Martian conditions |
title_fullStr |
Damage escape and repair in dried Chroococcidiopsis spp. from hot and cold deserts exposed to simulated space and Martian conditions |
title_full_unstemmed |
Damage escape and repair in dried Chroococcidiopsis spp. from hot and cold deserts exposed to simulated space and Martian conditions |
title_sort |
damage escape and repair in dried chroococcidiopsis spp. from hot and cold deserts exposed to simulated space and martian conditions |
publisher |
Mary Ann Liebert Incorporated |
publishDate |
2011 |
url |
http://hdl.handle.net/2108/13681 https://doi.org/10.1089/ast.2009.0430 |
long_lat |
ENVELOPE(160.650,160.650,-77.817,-77.817) |
geographic |
Antarctic Beacon Valley |
geographic_facet |
Antarctic Beacon Valley |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/21294638 info:eu-repo/semantics/altIdentifier/wos/WOS:000287689200007 volume:11 issue:1 firstpage:65 lastpage:73 numberofpages:9 journal:ASTROBIOLOGY http://hdl.handle.net/2108/13681 doi:10.1089/ast.2009.0430 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-79952019471 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1089/ast.2009.0430 |
container_title |
Astrobiology |
container_volume |
11 |
container_issue |
1 |
container_start_page |
65 |
op_container_end_page |
73 |
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1792045710119534592 |