Recombinant expression of Toluene o-Xylene Monooxygenase (ToMO) from Pseudomonas stutzeri OX1 in the marine Antarctic bacterium Pseudoalteromonas haloplanktis TAC125

• Published in: Journal of Biotechnology
• Main Authors: Siani, L, Di Donato, A, Sannia, G., PAPA, Rosanna
• Other Authors: Papa, Rosanna
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2006
• Subjects: Antarctic; Tac; Antarc*
• Online Access: http://hdl.handle.net/11573/899363; https://doi.org/10.1016/j.jbiotec.2006.04.027

The psychrophilic bacterium Pseudoalteromonas haloplanktis TAC125, isolated from Antarctic seawater, was used as recipient for a biodegradative gene of the mesophilic Pseudomonas stutzeri OX1. tou cluster, coding for Toluene o-Xylene Monooxygenase (ToMO), was successfully cloned and expressed into a "cold expression" vector. Apparent catalytic parameters of the recombinant microorganisms on three different substrates were determined and compared with those exhibited by Escherichia coli recombinant cells expressing ToMO. Production of a catalytically efficient TAC/tou microorganism supports the possibility of developing specific degradative capabilities for the bioremediation of chemically contaminated marine environments and of industrial effluents characterised by low temperatures.