Duplication of dystroglycan gene in most branches of teleost fish
BACKGROUND: The dystroglycan (DG) complex is a major non-integrin cell adhesion system whose multiple biological roles involve, among others, skeletal muscle stability, embryonic development and synapse maturation. DG is composed of two subunits: alpha-DG, extracellular and highly glycosylated, and...
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Online Access: | http://hdl.handle.net/11573/874467 https://doi.org/10.1186/1471-2199-8-34 |
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ftunivromairis:oai:iris.uniroma1.it:11573/874467 2024-04-14T08:03:22+00:00 Duplication of dystroglycan gene in most branches of teleost fish Pavoni Ernesto Cacchiarelli Davide Tittarelli Roberta Orsini Massimiliano Galtieri Antonio Giardina Bruno Brancaccio Andrea Pavoni, Ernesto Cacchiarelli, Davide Tittarelli, Roberta Orsini, Massimiliano Galtieri, Antonio Giardina, Bruno Brancaccio, Andrea 2007 ELETTRONICO http://hdl.handle.net/11573/874467 https://doi.org/10.1186/1471-2199-8-34 eng eng BioMed Central Ltd. place:London info:eu-repo/semantics/altIdentifier/pmid/17509131 info:eu-repo/semantics/altIdentifier/wos/WOS:000247018200001 volume:8 firstpage:1 lastpage:10 numberofpages:10 journal:BMC MOLECULAR BIOLOGY http://hdl.handle.net/11573/874467 doi:10.1186/1471-2199-8-34 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-34447503822 info:eu-repo/semantics/openAccess dystroglycan teleost fish western blot info:eu-repo/semantics/article 2007 ftunivromairis https://doi.org/10.1186/1471-2199-8-34 2024-03-21T19:12:12Z BACKGROUND: The dystroglycan (DG) complex is a major non-integrin cell adhesion system whose multiple biological roles involve, among others, skeletal muscle stability, embryonic development and synapse maturation. DG is composed of two subunits: alpha-DG, extracellular and highly glycosylated, and the transmembrane beta-DG, linking the cytoskeleton to the surrounding basement membrane in a wide variety of tissues. A single copy of the DG gene (DAG1) has been identified so far in humans and other mammals, encoding for a precursor protein which is post-translationally cleaved to liberate the two DG subunits. Similarly, D. rerio (zebrafish) seems to have a single copy of DAG1, whose removal was shown to cause a severe dystrophic phenotype in adult animals, although it is known that during evolution, due to a whole genome duplication (WGD) event, many teleost fish acquired multiple copies of several genes (paralogues). RESULTS: Data mining of pufferfish (T. nigroviridis and T. rubripes) and other teleost fish (O. latipes and G. aculeatus) available nucleotide sequences revealed the presence of two functional paralogous DG sequences. RT-PCR analysis proved that both the DG sequences are transcribed in T. nigroviridis. One of the two DG sequences harbours an additional mini-intronic sequence, 137 bp long, interrupting the uncomplicated exon-intron-exon pattern displayed by DAG1 in mammals and D. rerio. A similar scenario emerged also in D. labrax (sea bass), from whose genome we have cloned and sequenced a new DG sequence that also harbours a shorter additional intronic sequence of 116 bp. Western blot analysis confirmed the presence of DG protein products in all the species analysed including two teleost Antarctic species (T. bernacchii and C. hamatus). CONCLUSION: Our evolutionary analysis has shown that the whole-genome duplication event in the Class Actinopterygii (ray-finned fish) involved also DAG1. We unravelled new important molecular genetic details about fish orthologous DGs, which might help to increase ... Article in Journal/Newspaper Antarc* Antarctic Sapienza Università di Roma: CINECA IRIS Antarctic BMC Molecular Biology 8 1 34 |
institution |
Open Polar |
collection |
Sapienza Università di Roma: CINECA IRIS |
op_collection_id |
ftunivromairis |
language |
English |
topic |
dystroglycan teleost fish western blot |
spellingShingle |
dystroglycan teleost fish western blot Pavoni Ernesto Cacchiarelli Davide Tittarelli Roberta Orsini Massimiliano Galtieri Antonio Giardina Bruno Brancaccio Andrea Duplication of dystroglycan gene in most branches of teleost fish |
topic_facet |
dystroglycan teleost fish western blot |
description |
BACKGROUND: The dystroglycan (DG) complex is a major non-integrin cell adhesion system whose multiple biological roles involve, among others, skeletal muscle stability, embryonic development and synapse maturation. DG is composed of two subunits: alpha-DG, extracellular and highly glycosylated, and the transmembrane beta-DG, linking the cytoskeleton to the surrounding basement membrane in a wide variety of tissues. A single copy of the DG gene (DAG1) has been identified so far in humans and other mammals, encoding for a precursor protein which is post-translationally cleaved to liberate the two DG subunits. Similarly, D. rerio (zebrafish) seems to have a single copy of DAG1, whose removal was shown to cause a severe dystrophic phenotype in adult animals, although it is known that during evolution, due to a whole genome duplication (WGD) event, many teleost fish acquired multiple copies of several genes (paralogues). RESULTS: Data mining of pufferfish (T. nigroviridis and T. rubripes) and other teleost fish (O. latipes and G. aculeatus) available nucleotide sequences revealed the presence of two functional paralogous DG sequences. RT-PCR analysis proved that both the DG sequences are transcribed in T. nigroviridis. One of the two DG sequences harbours an additional mini-intronic sequence, 137 bp long, interrupting the uncomplicated exon-intron-exon pattern displayed by DAG1 in mammals and D. rerio. A similar scenario emerged also in D. labrax (sea bass), from whose genome we have cloned and sequenced a new DG sequence that also harbours a shorter additional intronic sequence of 116 bp. Western blot analysis confirmed the presence of DG protein products in all the species analysed including two teleost Antarctic species (T. bernacchii and C. hamatus). CONCLUSION: Our evolutionary analysis has shown that the whole-genome duplication event in the Class Actinopterygii (ray-finned fish) involved also DAG1. We unravelled new important molecular genetic details about fish orthologous DGs, which might help to increase ... |
author2 |
Pavoni, Ernesto Cacchiarelli, Davide Tittarelli, Roberta Orsini, Massimiliano Galtieri, Antonio Giardina, Bruno Brancaccio, Andrea |
format |
Article in Journal/Newspaper |
author |
Pavoni Ernesto Cacchiarelli Davide Tittarelli Roberta Orsini Massimiliano Galtieri Antonio Giardina Bruno Brancaccio Andrea |
author_facet |
Pavoni Ernesto Cacchiarelli Davide Tittarelli Roberta Orsini Massimiliano Galtieri Antonio Giardina Bruno Brancaccio Andrea |
author_sort |
Pavoni Ernesto |
title |
Duplication of dystroglycan gene in most branches of teleost fish |
title_short |
Duplication of dystroglycan gene in most branches of teleost fish |
title_full |
Duplication of dystroglycan gene in most branches of teleost fish |
title_fullStr |
Duplication of dystroglycan gene in most branches of teleost fish |
title_full_unstemmed |
Duplication of dystroglycan gene in most branches of teleost fish |
title_sort |
duplication of dystroglycan gene in most branches of teleost fish |
publisher |
BioMed Central Ltd. |
publishDate |
2007 |
url |
http://hdl.handle.net/11573/874467 https://doi.org/10.1186/1471-2199-8-34 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/17509131 info:eu-repo/semantics/altIdentifier/wos/WOS:000247018200001 volume:8 firstpage:1 lastpage:10 numberofpages:10 journal:BMC MOLECULAR BIOLOGY http://hdl.handle.net/11573/874467 doi:10.1186/1471-2199-8-34 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-34447503822 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1186/1471-2199-8-34 |
container_title |
BMC Molecular Biology |
container_volume |
8 |
container_issue |
1 |
container_start_page |
34 |
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