Aspartate aminotransferase from the antarctic bacterium Pseudoalteromonas haloplanktis TAC 125. Cloning, expression, properties, and molecular modelling

• Published in: European Journal of Biochemistry
• Main Authors: BIROLO L, TUTINO ML, FONTANELLA B, GERDAY C, MAINOLFI K, SANNIA G, VINCI F, MARINO G., PASCARELLA, Stefano
• Other Authors: Birolo, L, Tutino, Ml, Fontanella, B, Gerday, C, Mainolfi, K, Pascarella, Stefano, Sannia, G, Vinci, F, Marino, G.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Blackwell Science Limited:PO Box 88, Oxford OX2 0NE United Kingdom:011 44 1865 776868, 011 44 1865 206038, EMAIL: [e-mail], INTERNET: http://www.blackwell-science.com, Fax: [phone] 2000
• Subjects: aspartate aminotransferase; cold adaptation; psychrophile; Antarctic; Tac; The Antarctic; Antarc*
• Online Access: http://hdl.handle.net/11573/68485; https://doi.org/10.1046/j.1432-1327.2000.01299.x; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000087108400038&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a; http://onlinelibrary.wiley.com/doi/10.1046/j.1432-1327.2000.01299.x/pdf; http://www.scopus.com/inward/record.url?eid=2-s2.0-0008298889&partnerID=65&md5=183f8440f1beab9b9650497505b1a550

The gene encoding aspartate aminotransferase from the psychrophilic bacterium Pseudoalteromonas haloplanktis TAC 125 was cloned, sequenced and overexpressed in Escherichia coli. The recombinant protein (PhAspAT) was characterized both at the structural and functional level in comparison with the E....