Black coats in an admixed wolf x dog pack is melanism an indicator of hybridization in wolves?
The use of functional mutations, in addition to standard noncoding molecular markers, can help to detect hybridization and gene introgression in wild canid populations. We analyzed ancestry of a canid pack breeding in Central Italy that showed black coats and other unusual morphological traits sugge...
Published in: | European Journal of Wildlife Research |
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Online Access: | http://hdl.handle.net/11573/624589 https://doi.org/10.1007/s10344-013-0703-1 |
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ftunivromairis:oai:iris.uniroma1.it:11573/624589 2024-04-14T08:10:13+00:00 Black coats in an admixed wolf x dog pack is melanism an indicator of hybridization in wolves? Caniglia, Romolo Fabbri, Elena Greco, Claudia Galaverni, Marco Manghi, Lorenzo Sforzi, Andrea Randi, Ettore BOITANI, Luigi Caniglia, Romolo Fabbri, Elena Greco, Claudia Galaverni, Marco Manghi, Lorenzo Boitani, Luigi Sforzi, Andrea Randi, Ettore 2013 STAMPA http://hdl.handle.net/11573/624589 https://doi.org/10.1007/s10344-013-0703-1 eng eng SPRINGER info:eu-repo/semantics/altIdentifier/wos/WOS:000321866800009 volume:59 issue:4 firstpage:543 lastpage:555 numberofpages:13 journal:EUROPEAN JOURNAL OF WILDLIFE RESEARCH http://hdl.handle.net/11573/624589 doi:10.1007/s10344-013-0703-1 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84880510920 wolf × dog hybridization conservation genetics beta-defensin gene admixture analysis wolf x dog hybridization canis lupus coat color polymorphism β-defensin gene gene introgression info:eu-repo/semantics/article 2013 ftunivromairis https://doi.org/10.1007/s10344-013-0703-1 2024-03-21T19:20:30Z The use of functional mutations, in addition to standard noncoding molecular markers, can help to detect hybridization and gene introgression in wild canid populations. We analyzed ancestry of a canid pack breeding in Central Italy that showed black coats and other unusual morphological traits suggesting wolf x dog hybrid origins. Individuals were identified by genotyping excremental DNA at 13 autosomal microsatellites, mtDNA control region sequences, a male-specific restriction site on the ZFX/Y gene to determine the gender of the individuals, four Y-linked microsatellites to determine male haplotypes, and two melanistic mutations: a SNP at exon 4 of the Agouti locus and a 3-bp deletion at a beta-Defensin gene, the K locus. Results showed that: (1) the pack was founded by a single breeding pair of related individuals, probably brother and sister, and no immigrant was detected; (2) parents and offspring showed signals of admixture at autosomal microsatellites; and (3) the melanistic K locus deletion was present in the black-coated female parent and in 8/14 offspring, but it was absent in the wild type male parent. This deletion was found also in 17/40 village dogs randomly sampled from nearby areas, but it was absent in a random sample of 40 Italian wolves. These findings suggest that the pack received the K locus deletion from dogs. Admixture analyses of empirical and simulated genotypes indicate the parents of the pack originated through a single hybridization event at least two generations back. Genetic and phenotypic assessments of coat color mutations can contribute to investigation of the origin and dynamics of functional polymorphisms in hybridizing wolf populations and to develop appropriate guidelines to contrast hybridization with their domesticated relatives. Article in Journal/Newspaper Canis lupus Sapienza Università di Roma: CINECA IRIS European Journal of Wildlife Research 59 4 543 555 |
institution |
Open Polar |
collection |
Sapienza Università di Roma: CINECA IRIS |
op_collection_id |
ftunivromairis |
language |
English |
topic |
wolf × dog hybridization conservation genetics beta-defensin gene admixture analysis wolf x dog hybridization canis lupus coat color polymorphism β-defensin gene gene introgression |
spellingShingle |
wolf × dog hybridization conservation genetics beta-defensin gene admixture analysis wolf x dog hybridization canis lupus coat color polymorphism β-defensin gene gene introgression Caniglia, Romolo Fabbri, Elena Greco, Claudia Galaverni, Marco Manghi, Lorenzo Sforzi, Andrea Randi, Ettore BOITANI, Luigi Black coats in an admixed wolf x dog pack is melanism an indicator of hybridization in wolves? |
topic_facet |
wolf × dog hybridization conservation genetics beta-defensin gene admixture analysis wolf x dog hybridization canis lupus coat color polymorphism β-defensin gene gene introgression |
description |
The use of functional mutations, in addition to standard noncoding molecular markers, can help to detect hybridization and gene introgression in wild canid populations. We analyzed ancestry of a canid pack breeding in Central Italy that showed black coats and other unusual morphological traits suggesting wolf x dog hybrid origins. Individuals were identified by genotyping excremental DNA at 13 autosomal microsatellites, mtDNA control region sequences, a male-specific restriction site on the ZFX/Y gene to determine the gender of the individuals, four Y-linked microsatellites to determine male haplotypes, and two melanistic mutations: a SNP at exon 4 of the Agouti locus and a 3-bp deletion at a beta-Defensin gene, the K locus. Results showed that: (1) the pack was founded by a single breeding pair of related individuals, probably brother and sister, and no immigrant was detected; (2) parents and offspring showed signals of admixture at autosomal microsatellites; and (3) the melanistic K locus deletion was present in the black-coated female parent and in 8/14 offspring, but it was absent in the wild type male parent. This deletion was found also in 17/40 village dogs randomly sampled from nearby areas, but it was absent in a random sample of 40 Italian wolves. These findings suggest that the pack received the K locus deletion from dogs. Admixture analyses of empirical and simulated genotypes indicate the parents of the pack originated through a single hybridization event at least two generations back. Genetic and phenotypic assessments of coat color mutations can contribute to investigation of the origin and dynamics of functional polymorphisms in hybridizing wolf populations and to develop appropriate guidelines to contrast hybridization with their domesticated relatives. |
author2 |
Caniglia, Romolo Fabbri, Elena Greco, Claudia Galaverni, Marco Manghi, Lorenzo Boitani, Luigi Sforzi, Andrea Randi, Ettore |
format |
Article in Journal/Newspaper |
author |
Caniglia, Romolo Fabbri, Elena Greco, Claudia Galaverni, Marco Manghi, Lorenzo Sforzi, Andrea Randi, Ettore BOITANI, Luigi |
author_facet |
Caniglia, Romolo Fabbri, Elena Greco, Claudia Galaverni, Marco Manghi, Lorenzo Sforzi, Andrea Randi, Ettore BOITANI, Luigi |
author_sort |
Caniglia, Romolo |
title |
Black coats in an admixed wolf x dog pack is melanism an indicator of hybridization in wolves? |
title_short |
Black coats in an admixed wolf x dog pack is melanism an indicator of hybridization in wolves? |
title_full |
Black coats in an admixed wolf x dog pack is melanism an indicator of hybridization in wolves? |
title_fullStr |
Black coats in an admixed wolf x dog pack is melanism an indicator of hybridization in wolves? |
title_full_unstemmed |
Black coats in an admixed wolf x dog pack is melanism an indicator of hybridization in wolves? |
title_sort |
black coats in an admixed wolf x dog pack is melanism an indicator of hybridization in wolves? |
publisher |
SPRINGER |
publishDate |
2013 |
url |
http://hdl.handle.net/11573/624589 https://doi.org/10.1007/s10344-013-0703-1 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000321866800009 volume:59 issue:4 firstpage:543 lastpage:555 numberofpages:13 journal:EUROPEAN JOURNAL OF WILDLIFE RESEARCH http://hdl.handle.net/11573/624589 doi:10.1007/s10344-013-0703-1 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84880510920 |
op_doi |
https://doi.org/10.1007/s10344-013-0703-1 |
container_title |
European Journal of Wildlife Research |
container_volume |
59 |
container_issue |
4 |
container_start_page |
543 |
op_container_end_page |
555 |
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1796307740564389888 |