Molecular identification and larval morphological description of Contracaecum pelagicum (Nematoda: Anisakidae) from the anchovy Engraulis anchoita (Engraulidae) and fish-eating birds from the Argentine North Patagonian Sea
Anisakids use invertebrates as paratenic and/or intermediate hosts as a basic feature of larval transmission. The third-stage larva usually develops in invertebrates which are prey items of finfish paratenic hosts. Contracaecum larvae molt twice inside the egg and hatch as free third-stage larvae en...
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ftunivromairis:oai:iris.uniroma1.it:11573/560696 2024-04-21T08:06:02+00:00 Molecular identification and larval morphological description of Contracaecum pelagicum (Nematoda: Anisakidae) from the anchovy Engraulis anchoita (Engraulidae) and fish-eating birds from the Argentine North Patagonian Sea Lucas, E. Garbin MATTIUCCI, Simonetta Michela, Paoletti Julia, I. Diaz Giuseppe, Nascetti Graciela, T. Navone Lucas, E. Garbin Mattiucci, Simonetta Michela, Paoletti Julia, I. Diaz Giuseppe, Nascetti Graciela, T. Navone 2013 STAMPA http://hdl.handle.net/11573/560696 https://doi.org/10.1016/j.parint.2013.03.001 eng eng ELSEVIER IRELAND LTD info:eu-repo/semantics/altIdentifier/pmid/23500719 info:eu-repo/semantics/altIdentifier/wos/WOS:000318752400013 volume:62 issue:3 firstpage:309 lastpage:319 numberofpages:11 journal:PARASITOLOGY INTERNATIONAL http://hdl.handle.net/11573/560696 doi:10.1016/j.parint.2013.03.001 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84875389279 molecular marker patagonia engraulis anchoivita rrn mtdna cox2 larval transmission phalacrocorax atricep contracaecum pelagicum ecology its-1 its-2 info:eu-repo/semantics/article 2013 ftunivromairis https://doi.org/10.1016/j.parint.2013.03.001 2024-03-28T01:52:18Z Anisakids use invertebrates as paratenic and/or intermediate hosts as a basic feature of larval transmission. The third-stage larva usually develops in invertebrates which are prey items of finfish paratenic hosts. Contracaecum larvae molt twice inside the egg and hatch as free third-stage larvae ensheathed in the second-stage larval cuticle. Copepods act as paratenic or obligatory hosts, usually ingesting these free L3 larvae, and fish act as intermediate/paratenic or metaparatenic hosts preying on infected copepods. Fish-eating birds acquire L3 larvae by ingesting infected fish where they develop into the fourth-stage larvae and adults. Objectives of this work were to establish the specific correspondence between Contracaecum pelagicum L3 larvae parasitizing the anchovy Engraulis anchoita, and the adults parasitizing the Magellanic penguin Spheniscus magellanicus and the Imperial shag Phalacrocorax atriceps through the use of molecular markers; and, to evaluate the anisakid L3 larval recruitment and infection caused by ingestion of anchovy by S. magellanicus. Sixteen specimens of Contracaecum L3 larvae were analyzed from E. anchoita from Bahia Engano, Chubut, eight adult nematodes from S. magellanicus and six adult specimens from P. atriceps both from the Valdes Peninsula, Chubut. All nematodes were sequenced for three genes: mitochondrial cytochrome oxidase 2 (mtDNA cox2), mitochondrial ribosomal RNA (rrnS), and the internal transcribed spacers (ITS-1 and ITS-2) of the nuclear ribosomal DNA region. Phylogenetic analyses were performed by using Maximum Parsimony (MP) analysis by PAUP. In addition, studies under SEM and LM were carried out on L3 larvae. All L3 individuals from E. anchoita, adults from S. magellanicus, and P. atriceps clustered in the same clade, well supported in the MP tree inferred from the mtDNA cox2, and rrnS gene sequences analyses. Further, the sequence alignments of L3 larvae and adults of C pelagicum here obtained at the ITS-1 and ITS-2 regions of the rDNA matched the sequences of C ... Article in Journal/Newspaper Imperial Shag Phalacrocorax atriceps Copepods Sapienza Università di Roma: CINECA IRIS Parasitology International 62 3 309 319 |
institution |
Open Polar |
collection |
Sapienza Università di Roma: CINECA IRIS |
op_collection_id |
ftunivromairis |
language |
English |
topic |
molecular marker patagonia engraulis anchoivita rrn mtdna cox2 larval transmission phalacrocorax atricep contracaecum pelagicum ecology its-1 its-2 |
spellingShingle |
molecular marker patagonia engraulis anchoivita rrn mtdna cox2 larval transmission phalacrocorax atricep contracaecum pelagicum ecology its-1 its-2 Lucas, E. Garbin MATTIUCCI, Simonetta Michela, Paoletti Julia, I. Diaz Giuseppe, Nascetti Graciela, T. Navone Molecular identification and larval morphological description of Contracaecum pelagicum (Nematoda: Anisakidae) from the anchovy Engraulis anchoita (Engraulidae) and fish-eating birds from the Argentine North Patagonian Sea |
topic_facet |
molecular marker patagonia engraulis anchoivita rrn mtdna cox2 larval transmission phalacrocorax atricep contracaecum pelagicum ecology its-1 its-2 |
description |
Anisakids use invertebrates as paratenic and/or intermediate hosts as a basic feature of larval transmission. The third-stage larva usually develops in invertebrates which are prey items of finfish paratenic hosts. Contracaecum larvae molt twice inside the egg and hatch as free third-stage larvae ensheathed in the second-stage larval cuticle. Copepods act as paratenic or obligatory hosts, usually ingesting these free L3 larvae, and fish act as intermediate/paratenic or metaparatenic hosts preying on infected copepods. Fish-eating birds acquire L3 larvae by ingesting infected fish where they develop into the fourth-stage larvae and adults. Objectives of this work were to establish the specific correspondence between Contracaecum pelagicum L3 larvae parasitizing the anchovy Engraulis anchoita, and the adults parasitizing the Magellanic penguin Spheniscus magellanicus and the Imperial shag Phalacrocorax atriceps through the use of molecular markers; and, to evaluate the anisakid L3 larval recruitment and infection caused by ingestion of anchovy by S. magellanicus. Sixteen specimens of Contracaecum L3 larvae were analyzed from E. anchoita from Bahia Engano, Chubut, eight adult nematodes from S. magellanicus and six adult specimens from P. atriceps both from the Valdes Peninsula, Chubut. All nematodes were sequenced for three genes: mitochondrial cytochrome oxidase 2 (mtDNA cox2), mitochondrial ribosomal RNA (rrnS), and the internal transcribed spacers (ITS-1 and ITS-2) of the nuclear ribosomal DNA region. Phylogenetic analyses were performed by using Maximum Parsimony (MP) analysis by PAUP. In addition, studies under SEM and LM were carried out on L3 larvae. All L3 individuals from E. anchoita, adults from S. magellanicus, and P. atriceps clustered in the same clade, well supported in the MP tree inferred from the mtDNA cox2, and rrnS gene sequences analyses. Further, the sequence alignments of L3 larvae and adults of C pelagicum here obtained at the ITS-1 and ITS-2 regions of the rDNA matched the sequences of C ... |
author2 |
Lucas, E. Garbin Mattiucci, Simonetta Michela, Paoletti Julia, I. Diaz Giuseppe, Nascetti Graciela, T. Navone |
format |
Article in Journal/Newspaper |
author |
Lucas, E. Garbin MATTIUCCI, Simonetta Michela, Paoletti Julia, I. Diaz Giuseppe, Nascetti Graciela, T. Navone |
author_facet |
Lucas, E. Garbin MATTIUCCI, Simonetta Michela, Paoletti Julia, I. Diaz Giuseppe, Nascetti Graciela, T. Navone |
author_sort |
Lucas, E. Garbin |
title |
Molecular identification and larval morphological description of Contracaecum pelagicum (Nematoda: Anisakidae) from the anchovy Engraulis anchoita (Engraulidae) and fish-eating birds from the Argentine North Patagonian Sea |
title_short |
Molecular identification and larval morphological description of Contracaecum pelagicum (Nematoda: Anisakidae) from the anchovy Engraulis anchoita (Engraulidae) and fish-eating birds from the Argentine North Patagonian Sea |
title_full |
Molecular identification and larval morphological description of Contracaecum pelagicum (Nematoda: Anisakidae) from the anchovy Engraulis anchoita (Engraulidae) and fish-eating birds from the Argentine North Patagonian Sea |
title_fullStr |
Molecular identification and larval morphological description of Contracaecum pelagicum (Nematoda: Anisakidae) from the anchovy Engraulis anchoita (Engraulidae) and fish-eating birds from the Argentine North Patagonian Sea |
title_full_unstemmed |
Molecular identification and larval morphological description of Contracaecum pelagicum (Nematoda: Anisakidae) from the anchovy Engraulis anchoita (Engraulidae) and fish-eating birds from the Argentine North Patagonian Sea |
title_sort |
molecular identification and larval morphological description of contracaecum pelagicum (nematoda: anisakidae) from the anchovy engraulis anchoita (engraulidae) and fish-eating birds from the argentine north patagonian sea |
publisher |
ELSEVIER IRELAND LTD |
publishDate |
2013 |
url |
http://hdl.handle.net/11573/560696 https://doi.org/10.1016/j.parint.2013.03.001 |
genre |
Imperial Shag Phalacrocorax atriceps Copepods |
genre_facet |
Imperial Shag Phalacrocorax atriceps Copepods |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/23500719 info:eu-repo/semantics/altIdentifier/wos/WOS:000318752400013 volume:62 issue:3 firstpage:309 lastpage:319 numberofpages:11 journal:PARASITOLOGY INTERNATIONAL http://hdl.handle.net/11573/560696 doi:10.1016/j.parint.2013.03.001 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84875389279 |
op_doi |
https://doi.org/10.1016/j.parint.2013.03.001 |
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