Engineering Ascaris hemoglobin oxygen affinity in sperm whale myoglobin: role of tyrosine B10.
The contribution to oxygen stabilization of a tyrosine residue in topological position (B10) has been studied in sperm whale myoglobin by simultaneous replacement of residues at positions (B10), (E7) and (E10) as suggested by analysis of the sequence of high oxygen affinity hemoglobins, such as that...
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Language: | English |
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ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
1994
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Online Access: | http://hdl.handle.net/11573/406543 https://doi.org/10.1016/0014-5793(94)00918-X http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=A1994PX37600035&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a http://www.scopus.com/inward/record.url?eid=2-s2.0-0028092844&partnerID=65&md5=da451617e01bd63c1ebccad660fbb48f |
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ftunivromairis:oai:iris.uniroma1.it:11573/406543 2024-02-27T08:45:41+00:00 Engineering Ascaris hemoglobin oxygen affinity in sperm whale myoglobin: role of tyrosine B10. TRAVAGLINI ALLOCATELLI, Carlo CUTRUZZOLA', Francesca VALLONE, Beatrice BRUNORI, Maurizio Brancaccio A TRAVAGLINI ALLOCATELLI, Carlo Cutruzzola', Francesca Brancaccio, A Vallone, Beatrice Brunori, Maurizio 1994 STAMPA http://hdl.handle.net/11573/406543 https://doi.org/10.1016/0014-5793(94)00918-X http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=A1994PX37600035&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a http://www.scopus.com/inward/record.url?eid=2-s2.0-0028092844&partnerID=65&md5=da451617e01bd63c1ebccad660fbb48f eng eng ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS info:eu-repo/semantics/altIdentifier/pmid/7925944 info:eu-repo/semantics/altIdentifier/wos/WOS:A1994PX37600035 volume:352 firstpage:63 lastpage:66 numberofpages:4 journal:FEBS LETTERS http://hdl.handle.net/11573/406543 doi:10.1016/0014-5793(94)00918-X info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0028092844 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=A1994PX37600035&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a http://www.scopus.com/inward/record.url?eid=2-s2.0-0028092844&partnerID=65&md5=da451617e01bd63c1ebccad660fbb48f info:eu-repo/semantics/article 1994 ftunivromairis https://doi.org/10.1016/0014-5793(94)00918-X 2024-01-31T17:48:13Z The contribution to oxygen stabilization of a tyrosine residue in topological position (B10) has been studied in sperm whale myoglobin by simultaneous replacement of residues at positions (B10), (E7) and (E10) as suggested by analysis of the sequence of high oxygen affinity hemoglobins, such as that of the nematode Ascaris suum. Kinetic and equilibrium experiments with the gaseous ligands oxygen and carbon monoxide show that indeed the introduction of tyrosine (B10), together with replacement of the distal histidine (E7) with glutamine, is associated with a large decrease in the oxygen dissociation rate constant. Our results are consistent with the possible formation in the distal pocket of two hydrogen bonds with the iron-bound oxygen Article in Journal/Newspaper Sperm whale Sapienza UniversitĂ di Roma: CINECA IRIS FEBS Letters 352 1 63 66 |
institution |
Open Polar |
collection |
Sapienza UniversitĂ di Roma: CINECA IRIS |
op_collection_id |
ftunivromairis |
language |
English |
description |
The contribution to oxygen stabilization of a tyrosine residue in topological position (B10) has been studied in sperm whale myoglobin by simultaneous replacement of residues at positions (B10), (E7) and (E10) as suggested by analysis of the sequence of high oxygen affinity hemoglobins, such as that of the nematode Ascaris suum. Kinetic and equilibrium experiments with the gaseous ligands oxygen and carbon monoxide show that indeed the introduction of tyrosine (B10), together with replacement of the distal histidine (E7) with glutamine, is associated with a large decrease in the oxygen dissociation rate constant. Our results are consistent with the possible formation in the distal pocket of two hydrogen bonds with the iron-bound oxygen |
author2 |
TRAVAGLINI ALLOCATELLI, Carlo Cutruzzola', Francesca Brancaccio, A Vallone, Beatrice Brunori, Maurizio |
format |
Article in Journal/Newspaper |
author |
TRAVAGLINI ALLOCATELLI, Carlo CUTRUZZOLA', Francesca VALLONE, Beatrice BRUNORI, Maurizio Brancaccio A |
spellingShingle |
TRAVAGLINI ALLOCATELLI, Carlo CUTRUZZOLA', Francesca VALLONE, Beatrice BRUNORI, Maurizio Brancaccio A Engineering Ascaris hemoglobin oxygen affinity in sperm whale myoglobin: role of tyrosine B10. |
author_facet |
TRAVAGLINI ALLOCATELLI, Carlo CUTRUZZOLA', Francesca VALLONE, Beatrice BRUNORI, Maurizio Brancaccio A |
author_sort |
TRAVAGLINI ALLOCATELLI, Carlo |
title |
Engineering Ascaris hemoglobin oxygen affinity in sperm whale myoglobin: role of tyrosine B10. |
title_short |
Engineering Ascaris hemoglobin oxygen affinity in sperm whale myoglobin: role of tyrosine B10. |
title_full |
Engineering Ascaris hemoglobin oxygen affinity in sperm whale myoglobin: role of tyrosine B10. |
title_fullStr |
Engineering Ascaris hemoglobin oxygen affinity in sperm whale myoglobin: role of tyrosine B10. |
title_full_unstemmed |
Engineering Ascaris hemoglobin oxygen affinity in sperm whale myoglobin: role of tyrosine B10. |
title_sort |
engineering ascaris hemoglobin oxygen affinity in sperm whale myoglobin: role of tyrosine b10. |
publisher |
ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS |
publishDate |
1994 |
url |
http://hdl.handle.net/11573/406543 https://doi.org/10.1016/0014-5793(94)00918-X http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=A1994PX37600035&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a http://www.scopus.com/inward/record.url?eid=2-s2.0-0028092844&partnerID=65&md5=da451617e01bd63c1ebccad660fbb48f |
genre |
Sperm whale |
genre_facet |
Sperm whale |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/7925944 info:eu-repo/semantics/altIdentifier/wos/WOS:A1994PX37600035 volume:352 firstpage:63 lastpage:66 numberofpages:4 journal:FEBS LETTERS http://hdl.handle.net/11573/406543 doi:10.1016/0014-5793(94)00918-X info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0028092844 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=A1994PX37600035&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a http://www.scopus.com/inward/record.url?eid=2-s2.0-0028092844&partnerID=65&md5=da451617e01bd63c1ebccad660fbb48f |
op_doi |
https://doi.org/10.1016/0014-5793(94)00918-X |
container_title |
FEBS Letters |
container_volume |
352 |
container_issue |
1 |
container_start_page |
63 |
op_container_end_page |
66 |
_version_ |
1792054974305271808 |