Analysis of a cDNA sequence encoding the immunoglobulin heavy chain of the Antarctic teleost Trematomus bernacchii
A spleen cDNA library was constructed from the Antarctic teleost Trematomus bernacchii and immunoscreened with rabbit IgG specific for T. bernacchii Ig heavy chain. Eleven cDNA clones, varying in size and encoding the entire heavy chain or parts of it, were isolated. Here the complete nucleotide and...
Published in: | Fish & Shellfish Immunology |
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Format: | Article in Journal/Newspaper |
Language: | English |
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ACADEMIC PRESS LTD
2000
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Online Access: | http://hdl.handle.net/11573/398554 https://doi.org/10.1006/fsim.1999.0244 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000087288600005&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a http://www.scopus.com/inward/record.url?eid=2-s2.0-0034179062&partnerID=65&md5=d6eb279b3d4e2529512da29ace768b4e |
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ftunivromairis:oai:iris.uniroma1.it:11573/398554 2024-02-04T09:56:02+01:00 Analysis of a cDNA sequence encoding the immunoglobulin heavy chain of the Antarctic teleost Trematomus bernacchii Maria Rosaria Coscia Veronica Morea Umberto Oreste TRAMONTANO, ANNA Maria Rosaria, Coscia Veronica, Morea Tramontano, Anna Umberto, Oreste 2000 http://hdl.handle.net/11573/398554 https://doi.org/10.1006/fsim.1999.0244 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000087288600005&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a http://www.scopus.com/inward/record.url?eid=2-s2.0-0034179062&partnerID=65&md5=d6eb279b3d4e2529512da29ace768b4e eng eng ACADEMIC PRESS LTD info:eu-repo/semantics/altIdentifier/pmid/10938744 info:eu-repo/semantics/altIdentifier/wos/WOS:000087288600005 volume:10 issue:4 firstpage:343 lastpage:357 numberofpages:15 journal:FISH AND SHELLFISH IMMUNOLOGY http://hdl.handle.net/11573/398554 doi:10.1006/fsim.1999.0244 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0034179062 http://dx.doi.org/10.1006/fsim.1999.0244 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000087288600005&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a http://www.scopus.com/inward/record.url?eid=2-s2.0-0034179062&partnerID=65&md5=d6eb279b3d4e2529512da29ace768b4e amino acid sequence animal base sequence chemistry cold temperature cold-adapted protein complementary computer-assisted dna fish igh chain fishe gene library genetic ig hinge region image processing immunoglobulin heavy chain immunoglobulin m model molecular molecular sequence data nototheniidae polar fish rabbit structure-activity relationship t. bernacchii cdna library info:eu-repo/semantics/article 2000 ftunivromairis https://doi.org/10.1006/fsim.1999.0244 2024-01-10T18:15:13Z A spleen cDNA library was constructed from the Antarctic teleost Trematomus bernacchii and immunoscreened with rabbit IgG specific for T. bernacchii Ig heavy chain. Eleven cDNA clones, varying in size and encoding the entire heavy chain or parts of it, were isolated. Here the complete nucleotide and deduced amino acid sequences of clone 2C2 encoding the secretory IgH chain form are reported. Comparison of the amino acid sequence of the entire constant region of the T. bernacchii Ig heavy chain with those from other teleosts and two holostean fish showed percent identity ranging 53.6-60.6%, with the highest values found for Salmoniformes. The multiple sequence alignment revealed the presence of two remarkable insertions: one at the VH-CH1 boundary and a second one, not found in any other IgM heavy chain, localised at the CH2-CH3 boundary. The latter occurred in the region proposed to act as a 'hinge', and resulted in a CH2-CH3 hinge peptide longer than any other IgM hinge. Differences were also found in the number and position of putative N-glycosylation sites of the compared sequences. It is suggested that the unusual features found in the T. bernacchii Ig heavy chain might contribute to the flexibility of the Ig molecule and help understand more about the adaptation of Ig molecules to the polar sea environment. (C) 2000 Academic Press. Article in Journal/Newspaper Antarc* Antarctic Sapienza Università di Roma: CINECA IRIS Antarctic The Antarctic Fish & Shellfish Immunology 10 4 343 357 |
institution |
Open Polar |
collection |
Sapienza Università di Roma: CINECA IRIS |
op_collection_id |
ftunivromairis |
language |
English |
topic |
amino acid sequence animal base sequence chemistry cold temperature cold-adapted protein complementary computer-assisted dna fish igh chain fishe gene library genetic ig hinge region image processing immunoglobulin heavy chain immunoglobulin m model molecular molecular sequence data nototheniidae polar fish rabbit structure-activity relationship t. bernacchii cdna library |
spellingShingle |
amino acid sequence animal base sequence chemistry cold temperature cold-adapted protein complementary computer-assisted dna fish igh chain fishe gene library genetic ig hinge region image processing immunoglobulin heavy chain immunoglobulin m model molecular molecular sequence data nototheniidae polar fish rabbit structure-activity relationship t. bernacchii cdna library Maria Rosaria Coscia Veronica Morea Umberto Oreste TRAMONTANO, ANNA Analysis of a cDNA sequence encoding the immunoglobulin heavy chain of the Antarctic teleost Trematomus bernacchii |
topic_facet |
amino acid sequence animal base sequence chemistry cold temperature cold-adapted protein complementary computer-assisted dna fish igh chain fishe gene library genetic ig hinge region image processing immunoglobulin heavy chain immunoglobulin m model molecular molecular sequence data nototheniidae polar fish rabbit structure-activity relationship t. bernacchii cdna library |
description |
A spleen cDNA library was constructed from the Antarctic teleost Trematomus bernacchii and immunoscreened with rabbit IgG specific for T. bernacchii Ig heavy chain. Eleven cDNA clones, varying in size and encoding the entire heavy chain or parts of it, were isolated. Here the complete nucleotide and deduced amino acid sequences of clone 2C2 encoding the secretory IgH chain form are reported. Comparison of the amino acid sequence of the entire constant region of the T. bernacchii Ig heavy chain with those from other teleosts and two holostean fish showed percent identity ranging 53.6-60.6%, with the highest values found for Salmoniformes. The multiple sequence alignment revealed the presence of two remarkable insertions: one at the VH-CH1 boundary and a second one, not found in any other IgM heavy chain, localised at the CH2-CH3 boundary. The latter occurred in the region proposed to act as a 'hinge', and resulted in a CH2-CH3 hinge peptide longer than any other IgM hinge. Differences were also found in the number and position of putative N-glycosylation sites of the compared sequences. It is suggested that the unusual features found in the T. bernacchii Ig heavy chain might contribute to the flexibility of the Ig molecule and help understand more about the adaptation of Ig molecules to the polar sea environment. (C) 2000 Academic Press. |
author2 |
Maria Rosaria, Coscia Veronica, Morea Tramontano, Anna Umberto, Oreste |
format |
Article in Journal/Newspaper |
author |
Maria Rosaria Coscia Veronica Morea Umberto Oreste TRAMONTANO, ANNA |
author_facet |
Maria Rosaria Coscia Veronica Morea Umberto Oreste TRAMONTANO, ANNA |
author_sort |
Maria Rosaria Coscia |
title |
Analysis of a cDNA sequence encoding the immunoglobulin heavy chain of the Antarctic teleost Trematomus bernacchii |
title_short |
Analysis of a cDNA sequence encoding the immunoglobulin heavy chain of the Antarctic teleost Trematomus bernacchii |
title_full |
Analysis of a cDNA sequence encoding the immunoglobulin heavy chain of the Antarctic teleost Trematomus bernacchii |
title_fullStr |
Analysis of a cDNA sequence encoding the immunoglobulin heavy chain of the Antarctic teleost Trematomus bernacchii |
title_full_unstemmed |
Analysis of a cDNA sequence encoding the immunoglobulin heavy chain of the Antarctic teleost Trematomus bernacchii |
title_sort |
analysis of a cdna sequence encoding the immunoglobulin heavy chain of the antarctic teleost trematomus bernacchii |
publisher |
ACADEMIC PRESS LTD |
publishDate |
2000 |
url |
http://hdl.handle.net/11573/398554 https://doi.org/10.1006/fsim.1999.0244 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000087288600005&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a http://www.scopus.com/inward/record.url?eid=2-s2.0-0034179062&partnerID=65&md5=d6eb279b3d4e2529512da29ace768b4e |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/10938744 info:eu-repo/semantics/altIdentifier/wos/WOS:000087288600005 volume:10 issue:4 firstpage:343 lastpage:357 numberofpages:15 journal:FISH AND SHELLFISH IMMUNOLOGY http://hdl.handle.net/11573/398554 doi:10.1006/fsim.1999.0244 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0034179062 http://dx.doi.org/10.1006/fsim.1999.0244 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000087288600005&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a http://www.scopus.com/inward/record.url?eid=2-s2.0-0034179062&partnerID=65&md5=d6eb279b3d4e2529512da29ace768b4e |
op_doi |
https://doi.org/10.1006/fsim.1999.0244 |
container_title |
Fish & Shellfish Immunology |
container_volume |
10 |
container_issue |
4 |
container_start_page |
343 |
op_container_end_page |
357 |
_version_ |
1789960373090123776 |