Temperature acclimation modulates the oxygen binding properties of the Atlantic cod (Gadus morhua L.) genotypes - HbI*1/1, HbI*1/2, and HbI*2/2.

• Published in: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
• Main Authors: BRIX O, THORKILDSEN S, COLOSIMO, Alfredo
• Other Authors: Brix, O, Thorkildsen, S, Colosimo, Alfredo
• Format: Article in Journal/Newspaper
• Language: English
• Published: Elsevier Science Incorporated / NY Journals:Madison Square Station, PO Box 882:New York, NY 10159:[phone], EMAIL: [e-mail], INTERNET: http://www.elsevier.com, Fax: [phone] 2004
• Subjects: Atlantic cod; Temperature acclimation; Hemoglobin polymorphism; atlantic cod; Gadus morhua
• Online Access: http://hdl.handle.net/11573/26170; https://doi.org/10.1016/j.cbpb.2004.04.004; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000223255900014&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a; http://www.scopus.com/inward/record.url?eid=2-s2.0-3242740431&partnerID=65&md5=ef0fdcf758459fb33b54876e2c8a9f13

The influence of long-term acclimation temperatures in Atlantic cod (Gadus morhua) was studied by growth experiments carried out over a total of 272 individuals. The attention focused on the structural and functional modulation of the five electrophoretically distinguishable genotypes of cod hemoglobin (HbI*1/1, HbI*1/2, HbI*2/2, HbI*1/2b, and HbI*2/2b) and on the correlation with body length/weight. The main results can be summarized as follows. (1) Acclimation to lower (4 and 8 °C) and higher (12 and 15 °C) temperatures favors the expression of, respectively, more anodic and more cathodic hemoglobin components. (2) The optimal O2 transporting features are observed at 12 °C, as well as a saturation-dependent temperature dependence of O2 binding, which furthermore is strongly dependent upon the acclimation background. (3) The optimal growth condition for the three main genotypes (HbI*1/1, HbI*1/2, and HbI*2/2) is associated with T=12 °C. The overall results are consistent with the idea that environmental temperatures constitute a primary factor in the aggregation of individuals physiologically more than genetically homogeneous. This is fully confirmed by careful statistical analysis carried out over a subset of individuals for which the full set of structural (isoelectric focusing), functional (O2 binding), and growth data was available.