Construction and characterization of a chimeric myoglobin
In order to investigate the functional and structural role of modular structure in globins, we have engineered a chimeric myoglobin (ChimMb) in which the first and third exon come from the gene coding for the sperm whale Mb and the second exon from the gene coding for Aplysia limacina Mb. This ChimM...
Published in: | Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics |
---|---|
Main Authors: | , , , |
Other Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
ELSEVIER SCIENCE BV
2003
|
Subjects: | |
Online Access: | http://hdl.handle.net/11573/254858 https://doi.org/10.1016/s1570-9639(02)00528-9 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000180957900004&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a http://www.scopus.com/inward/record.url?eid=2-s2.0-1242341185&partnerID=65&md5=b6f38c20e2854398fba201899e702538 |
id |
ftunivromairis:oai:iris.uniroma1.it:11573/254858 |
---|---|
record_format |
openpolar |
spelling |
ftunivromairis:oai:iris.uniroma1.it:11573/254858 2024-04-14T08:20:06+00:00 Construction and characterization of a chimeric myoglobin Raffaella Musto BRUNORI, Maurizio CUTRUZZOLA', Francesca TRAVAGLINI ALLOCATELLI, Carlo Raffaella, Musto Brunori, Maurizio Cutruzzola', Francesca TRAVAGLINI ALLOCATELLI, Carlo 2003 http://hdl.handle.net/11573/254858 https://doi.org/10.1016/s1570-9639(02)00528-9 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000180957900004&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a http://www.scopus.com/inward/record.url?eid=2-s2.0-1242341185&partnerID=65&md5=b6f38c20e2854398fba201899e702538 eng eng ELSEVIER SCIENCE BV info:eu-repo/semantics/altIdentifier/pmid/12573243 info:eu-repo/semantics/altIdentifier/wos/WOS:000180957900004 volume:1645 issue:2 firstpage:139 lastpage:145 numberofpages:7 journal:BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS http://hdl.handle.net/11573/254858 doi:10.1016/s1570-9639(02)00528-9 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-1242341185 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000180957900004&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a http://www.scopus.com/inward/record.url?eid=2-s2.0-1242341185&partnerID=65&md5=b6f38c20e2854398fba201899e702538 aplysia-limacina myoglobin chimera folding folding intermediate molten globule myoglobin reactivity info:eu-repo/semantics/article 2003 ftunivromairis https://doi.org/10.1016/s1570-9639(02)00528-9 2024-03-21T18:42:40Z In order to investigate the functional and structural role of modular structure in globins, we have engineered a chimeric myoglobin (ChimMb) in which the first and third exon come from the gene coding for the sperm whale Mb and the second exon from the gene coding for Aplysia limacina Mb. This ChimMb, fused to the Maltose Binding Protein (MBP) and expressed in Escherichia coli as an apoprotein, binds protoheme in a 1:1 stoichiometric ratio. Based on some functional and spectroscopic properties, we conclude that the central core of the ChimMb (which derives from A. limacina) is native-like. On the other hand, the ChimMb deprived (by proteolytic digestion) of the fused MBP displays a considerably reduced stability. These results suggest that the sperm whale A-G-H nucleus does not contribute significantly to the overall stability of the ChimMb. (C) 2002 Elsevier Science B.V. All rights reserved. Article in Journal/Newspaper Sperm whale Sapienza Università di Roma: CINECA IRIS Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 1645 2 139 145 |
institution |
Open Polar |
collection |
Sapienza Università di Roma: CINECA IRIS |
op_collection_id |
ftunivromairis |
language |
English |
topic |
aplysia-limacina myoglobin chimera folding folding intermediate molten globule myoglobin reactivity |
spellingShingle |
aplysia-limacina myoglobin chimera folding folding intermediate molten globule myoglobin reactivity Raffaella Musto BRUNORI, Maurizio CUTRUZZOLA', Francesca TRAVAGLINI ALLOCATELLI, Carlo Construction and characterization of a chimeric myoglobin |
topic_facet |
aplysia-limacina myoglobin chimera folding folding intermediate molten globule myoglobin reactivity |
description |
In order to investigate the functional and structural role of modular structure in globins, we have engineered a chimeric myoglobin (ChimMb) in which the first and third exon come from the gene coding for the sperm whale Mb and the second exon from the gene coding for Aplysia limacina Mb. This ChimMb, fused to the Maltose Binding Protein (MBP) and expressed in Escherichia coli as an apoprotein, binds protoheme in a 1:1 stoichiometric ratio. Based on some functional and spectroscopic properties, we conclude that the central core of the ChimMb (which derives from A. limacina) is native-like. On the other hand, the ChimMb deprived (by proteolytic digestion) of the fused MBP displays a considerably reduced stability. These results suggest that the sperm whale A-G-H nucleus does not contribute significantly to the overall stability of the ChimMb. (C) 2002 Elsevier Science B.V. All rights reserved. |
author2 |
Raffaella, Musto Brunori, Maurizio Cutruzzola', Francesca TRAVAGLINI ALLOCATELLI, Carlo |
format |
Article in Journal/Newspaper |
author |
Raffaella Musto BRUNORI, Maurizio CUTRUZZOLA', Francesca TRAVAGLINI ALLOCATELLI, Carlo |
author_facet |
Raffaella Musto BRUNORI, Maurizio CUTRUZZOLA', Francesca TRAVAGLINI ALLOCATELLI, Carlo |
author_sort |
Raffaella Musto |
title |
Construction and characterization of a chimeric myoglobin |
title_short |
Construction and characterization of a chimeric myoglobin |
title_full |
Construction and characterization of a chimeric myoglobin |
title_fullStr |
Construction and characterization of a chimeric myoglobin |
title_full_unstemmed |
Construction and characterization of a chimeric myoglobin |
title_sort |
construction and characterization of a chimeric myoglobin |
publisher |
ELSEVIER SCIENCE BV |
publishDate |
2003 |
url |
http://hdl.handle.net/11573/254858 https://doi.org/10.1016/s1570-9639(02)00528-9 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000180957900004&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a http://www.scopus.com/inward/record.url?eid=2-s2.0-1242341185&partnerID=65&md5=b6f38c20e2854398fba201899e702538 |
genre |
Sperm whale |
genre_facet |
Sperm whale |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/12573243 info:eu-repo/semantics/altIdentifier/wos/WOS:000180957900004 volume:1645 issue:2 firstpage:139 lastpage:145 numberofpages:7 journal:BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS http://hdl.handle.net/11573/254858 doi:10.1016/s1570-9639(02)00528-9 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-1242341185 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000180957900004&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a http://www.scopus.com/inward/record.url?eid=2-s2.0-1242341185&partnerID=65&md5=b6f38c20e2854398fba201899e702538 |
op_doi |
https://doi.org/10.1016/s1570-9639(02)00528-9 |
container_title |
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics |
container_volume |
1645 |
container_issue |
2 |
container_start_page |
139 |
op_container_end_page |
145 |
_version_ |
1796298307756097536 |