Ferritin from the spleen of the Anctartic teleost Trematomus bernacchii is an M-type homopolymer
Ferritin from the spleen of the Antarctic teleost Trematomus bernacchii is composed of a single subunit that contains both the ferroxidase center residues, typical of mammalian H chains, and the carboxylate residues forming the micelle nucleation site, typical ofmammalianL chains.Comparison of the a...
Published in: | European Journal of Biochemistry |
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Main Authors: | , , , , , |
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Format: | Article in Journal/Newspaper |
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BLACKWELL PUBLISHING LTD, 9600 GARSINGTON RD, OXFORD OX4 2DG, OXON, ENGLAND
2002
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Online Access: | http://hdl.handle.net/11573/251998 https://doi.org/10.1046/j.1432-1327.2002.02762.x http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000174575200002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a http://www.scopus.com/inward/record.url?eid=2-s2.0-0036231083&partnerID=65&md5=03fcce759764aa678281739a728344b3 |
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ftunivromairis:oai:iris.uniroma1.it:11573/251998 2024-02-27T08:33:40+00:00 Ferritin from the spleen of the Anctartic teleost Trematomus bernacchii is an M-type homopolymer MIGNOGNA, Giuseppina CHIARALUCE, Roberta CONSALVI, Valerio CAVALLO, Stefano STEFANINI, Simonetta CHIANCONE, Emilia Mignogna, Giuseppina Chiaraluce, Roberta Consalvi, Valerio Cavallo, Stefano Stefanini, Simonetta Chiancone, Emilia 2002 STAMPA http://hdl.handle.net/11573/251998 https://doi.org/10.1046/j.1432-1327.2002.02762.x http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000174575200002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a http://www.scopus.com/inward/record.url?eid=2-s2.0-0036231083&partnerID=65&md5=03fcce759764aa678281739a728344b3 eng eng BLACKWELL PUBLISHING LTD, 9600 GARSINGTON RD, OXFORD OX4 2DG, OXON, ENGLAND info:eu-repo/semantics/altIdentifier/pmid/11895429 info:eu-repo/semantics/altIdentifier/wos/WOS:000174575200002 volume:269 firstpage:1600 lastpage:1606 numberofpages:7 journal:EUROPEAN JOURNAL OF BIOCHEMISTRY http://hdl.handle.net/11573/251998 doi:10.1046/j.1432-1327.2002.02762.x info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0036231083 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000174575200002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a http://www.scopus.com/inward/record.url?eid=2-s2.0-0036231083&partnerID=65&md5=03fcce759764aa678281739a728344b3 info:eu-repo/semantics/article 2002 ftunivromairis https://doi.org/10.1046/j.1432-1327.2002.02762.x 2024-01-31T17:50:37Z Ferritin from the spleen of the Antarctic teleost Trematomus bernacchii is composed of a single subunit that contains both the ferroxidase center residues, typical of mammalian H chains, and the carboxylate residues forming the micelle nucleation site, typical ofmammalianL chains.Comparison of the amino-acid sequence with those available from lower vertebrates indicates that T. bernacchii ferritin can be classi®ed as an M-type homopolymer. Interestingly, the T. bernacchii ferritin chain shows 85.7% identity with a cold- inducible ferritin chain of the rainbow trout Salmo gairdneri. The structural and functional properties indicate that cold acclimation and functional adaptation to low temperatures are achieved without signi®cant modi®cation of the protein stability. In fact, the stability of T. bernacchii ferritin to de- naturation induced by acid or temperature closely resembles that of mesophilic mammalian ferritins. Moreover iron is taken up e ciently and the activation energy of the reaction is 74.9 kJámol)1, a value slightly lower than that measured for the human recombinant H ferritin (80.8 kJámol)1). Article in Journal/Newspaper Antarc* Antarctic Sapienza Università di Roma: CINECA IRIS Antarctic The Antarctic European Journal of Biochemistry 269 6 1600 1606 |
institution |
Open Polar |
collection |
Sapienza Università di Roma: CINECA IRIS |
op_collection_id |
ftunivromairis |
language |
English |
description |
Ferritin from the spleen of the Antarctic teleost Trematomus bernacchii is composed of a single subunit that contains both the ferroxidase center residues, typical of mammalian H chains, and the carboxylate residues forming the micelle nucleation site, typical ofmammalianL chains.Comparison of the amino-acid sequence with those available from lower vertebrates indicates that T. bernacchii ferritin can be classi®ed as an M-type homopolymer. Interestingly, the T. bernacchii ferritin chain shows 85.7% identity with a cold- inducible ferritin chain of the rainbow trout Salmo gairdneri. The structural and functional properties indicate that cold acclimation and functional adaptation to low temperatures are achieved without signi®cant modi®cation of the protein stability. In fact, the stability of T. bernacchii ferritin to de- naturation induced by acid or temperature closely resembles that of mesophilic mammalian ferritins. Moreover iron is taken up e ciently and the activation energy of the reaction is 74.9 kJámol)1, a value slightly lower than that measured for the human recombinant H ferritin (80.8 kJámol)1). |
author2 |
Mignogna, Giuseppina Chiaraluce, Roberta Consalvi, Valerio Cavallo, Stefano Stefanini, Simonetta Chiancone, Emilia |
format |
Article in Journal/Newspaper |
author |
MIGNOGNA, Giuseppina CHIARALUCE, Roberta CONSALVI, Valerio CAVALLO, Stefano STEFANINI, Simonetta CHIANCONE, Emilia |
spellingShingle |
MIGNOGNA, Giuseppina CHIARALUCE, Roberta CONSALVI, Valerio CAVALLO, Stefano STEFANINI, Simonetta CHIANCONE, Emilia Ferritin from the spleen of the Anctartic teleost Trematomus bernacchii is an M-type homopolymer |
author_facet |
MIGNOGNA, Giuseppina CHIARALUCE, Roberta CONSALVI, Valerio CAVALLO, Stefano STEFANINI, Simonetta CHIANCONE, Emilia |
author_sort |
MIGNOGNA, Giuseppina |
title |
Ferritin from the spleen of the Anctartic teleost Trematomus bernacchii is an M-type homopolymer |
title_short |
Ferritin from the spleen of the Anctartic teleost Trematomus bernacchii is an M-type homopolymer |
title_full |
Ferritin from the spleen of the Anctartic teleost Trematomus bernacchii is an M-type homopolymer |
title_fullStr |
Ferritin from the spleen of the Anctartic teleost Trematomus bernacchii is an M-type homopolymer |
title_full_unstemmed |
Ferritin from the spleen of the Anctartic teleost Trematomus bernacchii is an M-type homopolymer |
title_sort |
ferritin from the spleen of the anctartic teleost trematomus bernacchii is an m-type homopolymer |
publisher |
BLACKWELL PUBLISHING LTD, 9600 GARSINGTON RD, OXFORD OX4 2DG, OXON, ENGLAND |
publishDate |
2002 |
url |
http://hdl.handle.net/11573/251998 https://doi.org/10.1046/j.1432-1327.2002.02762.x http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000174575200002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a http://www.scopus.com/inward/record.url?eid=2-s2.0-0036231083&partnerID=65&md5=03fcce759764aa678281739a728344b3 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/11895429 info:eu-repo/semantics/altIdentifier/wos/WOS:000174575200002 volume:269 firstpage:1600 lastpage:1606 numberofpages:7 journal:EUROPEAN JOURNAL OF BIOCHEMISTRY http://hdl.handle.net/11573/251998 doi:10.1046/j.1432-1327.2002.02762.x info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0036231083 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000174575200002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a http://www.scopus.com/inward/record.url?eid=2-s2.0-0036231083&partnerID=65&md5=03fcce759764aa678281739a728344b3 |
op_doi |
https://doi.org/10.1046/j.1432-1327.2002.02762.x |
container_title |
European Journal of Biochemistry |
container_volume |
269 |
container_issue |
6 |
container_start_page |
1600 |
op_container_end_page |
1606 |
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1792046895016706048 |