Molecular assay for the identification of Setaria tundra
We report the molecular characterization of the amplification products obtained by specific PCR experiments aimed to identify the 5S ribosomal spacer of Setaria tundra specimens isolated from roe deer (Capreolus capreolus) in north Italy, which represent the first record of the parasite in this coun...
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Elsevier BV:PO Box 211, 1000 AE Amsterdam Netherlands:011 31 20 4853757, 011 31 20 4853642, 011 31 20 4853641, EMAIL: nlinfo-f@elsevier.nl, INTERNET: http://www.elsevier.nl, Fax: 011 31 20 4853598
2003
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ftunivromairis:oai:iris.uniroma1.it:11573/23 2024-04-14T08:20:33+00:00 Molecular assay for the identification of Setaria tundra FAVIA G FERROGLIO E CASIRAGHI M RICCI I ROSSI L. CANCRINI, Gabriella Favia, G Cancrini, Gabriella Ferroglio, E Casiraghi, M Ricci, I Rossi, L. 2003 STAMPA http://hdl.handle.net/11573/23 eng eng Elsevier BV:PO Box 211, 1000 AE Amsterdam Netherlands:011 31 20 4853757, 011 31 20 4853642, 011 31 20 4853641, EMAIL: nlinfo-f@elsevier.nl, INTERNET: http://www.elsevier.nl, Fax: 011 31 20 4853598 info:eu-repo/semantics/altIdentifier/wos/WOS:000186831200015 volume:117 firstpage:139 lastpage:145 numberofpages:7 journal:VETERINARY PARASITOLOGY http://hdl.handle.net/11573/23 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0242298586 info:eu-repo/semantics/article 2003 ftunivromairis 2024-03-21T18:36:32Z We report the molecular characterization of the amplification products obtained by specific PCR experiments aimed to identify the 5S ribosomal spacer of Setaria tundra specimens isolated from roe deer (Capreolus capreolus) in north Italy, which represent the first record of the parasite in this country. Three different fragments of approximately 400, 800, and 1200 bp (base pairs) are produced. Sequence analyses showed that all three fragments share a very high level of similarity to 5S spacer sequences of some Setaria species and other filariae present in genebank. Based on these sequences, we were able to design species-specific PCR primers for the precise identification of S. tundra. Article in Journal/Newspaper Tundra Sapienza Università di Roma: CINECA IRIS |
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Open Polar |
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Sapienza Università di Roma: CINECA IRIS |
op_collection_id |
ftunivromairis |
language |
English |
description |
We report the molecular characterization of the amplification products obtained by specific PCR experiments aimed to identify the 5S ribosomal spacer of Setaria tundra specimens isolated from roe deer (Capreolus capreolus) in north Italy, which represent the first record of the parasite in this country. Three different fragments of approximately 400, 800, and 1200 bp (base pairs) are produced. Sequence analyses showed that all three fragments share a very high level of similarity to 5S spacer sequences of some Setaria species and other filariae present in genebank. Based on these sequences, we were able to design species-specific PCR primers for the precise identification of S. tundra. |
author2 |
Favia, G Cancrini, Gabriella Ferroglio, E Casiraghi, M Ricci, I Rossi, L. |
format |
Article in Journal/Newspaper |
author |
FAVIA G FERROGLIO E CASIRAGHI M RICCI I ROSSI L. CANCRINI, Gabriella |
spellingShingle |
FAVIA G FERROGLIO E CASIRAGHI M RICCI I ROSSI L. CANCRINI, Gabriella Molecular assay for the identification of Setaria tundra |
author_facet |
FAVIA G FERROGLIO E CASIRAGHI M RICCI I ROSSI L. CANCRINI, Gabriella |
author_sort |
FAVIA G |
title |
Molecular assay for the identification of Setaria tundra |
title_short |
Molecular assay for the identification of Setaria tundra |
title_full |
Molecular assay for the identification of Setaria tundra |
title_fullStr |
Molecular assay for the identification of Setaria tundra |
title_full_unstemmed |
Molecular assay for the identification of Setaria tundra |
title_sort |
molecular assay for the identification of setaria tundra |
publisher |
Elsevier BV:PO Box 211, 1000 AE Amsterdam Netherlands:011 31 20 4853757, 011 31 20 4853642, 011 31 20 4853641, EMAIL: nlinfo-f@elsevier.nl, INTERNET: http://www.elsevier.nl, Fax: 011 31 20 4853598 |
publishDate |
2003 |
url |
http://hdl.handle.net/11573/23 |
genre |
Tundra |
genre_facet |
Tundra |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000186831200015 volume:117 firstpage:139 lastpage:145 numberofpages:7 journal:VETERINARY PARASITOLOGY http://hdl.handle.net/11573/23 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0242298586 |
_version_ |
1796298885556076544 |