Polarized X-ray Absorption Near-Edge Structure Spectroscopy of Neuroglobin and Myoglobin Single Crystals
Polarized Fe K-edge X-ray absorption near-edge structure (XANES) spectra of murine carbonmonoxy-neuroglobin (NgbCO) single crystals have been collected and compared with a number of derivatives of sperm whale myoglobin (Mb), that is, the nitrosyl (MbNO) and deoxy (Mb) derivatives, the previously rep...
Published in: | The Journal of Physical Chemistry B |
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AMER CHEMICAL SOC
2010
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Online Access: | http://hdl.handle.net/11573/229475 https://doi.org/10.1021/jp104395g http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000282855100024&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a http://www.scopus.com/inward/record.url?eid=2-s2.0-77958026039&partnerID=65&md5=771e2f31ac0f7cdf477a8164db1a3d6e |
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ftunivromairis:oai:iris.uniroma1.it:11573/229475 2024-02-27T08:45:42+00:00 Polarized X-ray Absorption Near-Edge Structure Spectroscopy of Neuroglobin and Myoglobin Single Crystals Alessandro Arcovito Chiara Ardiccioni Michele Cianci Stefano Della Longa D'ANGELO, Paola VALLONE, Beatrice Alessandro, Arcovito Chiara, Ardiccioni Michele, Cianci D'Angelo, Paola Vallone, Beatrice Stefano Della, Longa 2010 STAMPA http://hdl.handle.net/11573/229475 https://doi.org/10.1021/jp104395g http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000282855100024&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a http://www.scopus.com/inward/record.url?eid=2-s2.0-77958026039&partnerID=65&md5=771e2f31ac0f7cdf477a8164db1a3d6e eng eng AMER CHEMICAL SOC info:eu-repo/semantics/altIdentifier/pmid/20879741 info:eu-repo/semantics/altIdentifier/wos/WOS:000282855100024 volume:114 issue:41 firstpage:13223 lastpage:13231 numberofpages:9 journal:JOURNAL OF PHYSICAL CHEMISTRY. B, CONDENSED MATTER, MATERIALS, SURFACES, INTERFACES & BIOPHYSICAL http://hdl.handle.net/11573/229475 doi:10.1021/jp104395g info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-77958026039 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000282855100024&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a http://www.scopus.com/inward/record.url?eid=2-s2.0-77958026039&partnerID=65&md5=771e2f31ac0f7cdf477a8164db1a3d6e info:eu-repo/semantics/article 2010 ftunivromairis https://doi.org/10.1021/jp104395g 2024-01-31T18:09:14Z Polarized Fe K-edge X-ray absorption near-edge structure (XANES) spectra of murine carbonmonoxy-neuroglobin (NgbCO) single crystals have been collected and compared with a number of derivatives of sperm whale myoglobin (Mb), that is, the nitrosyl (MbNO) and deoxy (Mb) derivatives, the previously reported cyanomet (MbCN) and carbonmonoxy (MbCO) derivatives, and the cryogenic photoproduct of MbCO (Mb center dot CO). The single crystals under study exhibit a strong XANES angular dichroism which allows the heme geometry of each sample to be analyzed with extremely high accuracy via the full multiple scattering (MS) approach. The results of two alternative methods to undergo the MS analysis have been compared with high resolution X-ray diffraction (XRD) data and with X-ray absorption spectroscopy (XAS) data in solution. As a result of the present analysis, the Fe-heme Structure in solution and in the cryo-trapped NgbCO single crystal (which cracks at room temperature) are the same. Accordingly, the residual energy involved in the protein relaxation responsible of crystal cracking at room temperature alter CO binding does not reside in the helm pocket. A combined approach (polarized XANES and XRD) is suggested to be applied on the same single crystals of metalloproteins at opportunely equipped synchrotron beamlines. Article in Journal/Newspaper Sperm whale Sapienza Università di Roma: CINECA IRIS The Journal of Physical Chemistry B 114 41 13223 13231 |
institution |
Open Polar |
collection |
Sapienza Università di Roma: CINECA IRIS |
op_collection_id |
ftunivromairis |
language |
English |
description |
Polarized Fe K-edge X-ray absorption near-edge structure (XANES) spectra of murine carbonmonoxy-neuroglobin (NgbCO) single crystals have been collected and compared with a number of derivatives of sperm whale myoglobin (Mb), that is, the nitrosyl (MbNO) and deoxy (Mb) derivatives, the previously reported cyanomet (MbCN) and carbonmonoxy (MbCO) derivatives, and the cryogenic photoproduct of MbCO (Mb center dot CO). The single crystals under study exhibit a strong XANES angular dichroism which allows the heme geometry of each sample to be analyzed with extremely high accuracy via the full multiple scattering (MS) approach. The results of two alternative methods to undergo the MS analysis have been compared with high resolution X-ray diffraction (XRD) data and with X-ray absorption spectroscopy (XAS) data in solution. As a result of the present analysis, the Fe-heme Structure in solution and in the cryo-trapped NgbCO single crystal (which cracks at room temperature) are the same. Accordingly, the residual energy involved in the protein relaxation responsible of crystal cracking at room temperature alter CO binding does not reside in the helm pocket. A combined approach (polarized XANES and XRD) is suggested to be applied on the same single crystals of metalloproteins at opportunely equipped synchrotron beamlines. |
author2 |
Alessandro, Arcovito Chiara, Ardiccioni Michele, Cianci D'Angelo, Paola Vallone, Beatrice Stefano Della, Longa |
format |
Article in Journal/Newspaper |
author |
Alessandro Arcovito Chiara Ardiccioni Michele Cianci Stefano Della Longa D'ANGELO, Paola VALLONE, Beatrice |
spellingShingle |
Alessandro Arcovito Chiara Ardiccioni Michele Cianci Stefano Della Longa D'ANGELO, Paola VALLONE, Beatrice Polarized X-ray Absorption Near-Edge Structure Spectroscopy of Neuroglobin and Myoglobin Single Crystals |
author_facet |
Alessandro Arcovito Chiara Ardiccioni Michele Cianci Stefano Della Longa D'ANGELO, Paola VALLONE, Beatrice |
author_sort |
Alessandro Arcovito |
title |
Polarized X-ray Absorption Near-Edge Structure Spectroscopy of Neuroglobin and Myoglobin Single Crystals |
title_short |
Polarized X-ray Absorption Near-Edge Structure Spectroscopy of Neuroglobin and Myoglobin Single Crystals |
title_full |
Polarized X-ray Absorption Near-Edge Structure Spectroscopy of Neuroglobin and Myoglobin Single Crystals |
title_fullStr |
Polarized X-ray Absorption Near-Edge Structure Spectroscopy of Neuroglobin and Myoglobin Single Crystals |
title_full_unstemmed |
Polarized X-ray Absorption Near-Edge Structure Spectroscopy of Neuroglobin and Myoglobin Single Crystals |
title_sort |
polarized x-ray absorption near-edge structure spectroscopy of neuroglobin and myoglobin single crystals |
publisher |
AMER CHEMICAL SOC |
publishDate |
2010 |
url |
http://hdl.handle.net/11573/229475 https://doi.org/10.1021/jp104395g http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000282855100024&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a http://www.scopus.com/inward/record.url?eid=2-s2.0-77958026039&partnerID=65&md5=771e2f31ac0f7cdf477a8164db1a3d6e |
genre |
Sperm whale |
genre_facet |
Sperm whale |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/20879741 info:eu-repo/semantics/altIdentifier/wos/WOS:000282855100024 volume:114 issue:41 firstpage:13223 lastpage:13231 numberofpages:9 journal:JOURNAL OF PHYSICAL CHEMISTRY. B, CONDENSED MATTER, MATERIALS, SURFACES, INTERFACES & BIOPHYSICAL http://hdl.handle.net/11573/229475 doi:10.1021/jp104395g info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-77958026039 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000282855100024&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a http://www.scopus.com/inward/record.url?eid=2-s2.0-77958026039&partnerID=65&md5=771e2f31ac0f7cdf477a8164db1a3d6e |
op_doi |
https://doi.org/10.1021/jp104395g |
container_title |
The Journal of Physical Chemistry B |
container_volume |
114 |
container_issue |
41 |
container_start_page |
13223 |
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13231 |
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