Cold-azurin, a new antibiofilm protein produced by the antarctic marine bacterium pseudomonas sp. tae6080
Biofilm is accountable for nosocomial infections and chronic illness, making it a serious economic and public health problem. Staphylococcus epidermidis, thanks to its ability to form biofilm and colonize biomaterials, represents the most frequent causative agent involved in biofilm-associated infec...
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Language: | English |
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2024
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Online Access: | https://hdl.handle.net/11573/1702307 https://doi.org/10.3390/md22020061 |
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ftunivromairis:oai:iris.uniroma1.it:11573/1702307 2024-04-21T07:52:55+00:00 Cold-azurin, a new antibiofilm protein produced by the antarctic marine bacterium pseudomonas sp. tae6080 Caterina D’Angelo Marika Trecca Andrea Carpentieri Marco Artini Laura Selan Maria Luisa Tutino Rosanna Papa Ermenegilda Parrilli D’Angelo, Caterina Trecca, Marika Carpentieri, Andrea Artini, Marco Selan, Laura Luisa Tutino, Maria Papa, Rosanna Parrilli, Ermenegilda 2024 https://hdl.handle.net/11573/1702307 https://doi.org/10.3390/md22020061 eng eng info:eu-repo/semantics/altIdentifier/pmid/38393032 info:eu-repo/semantics/altIdentifier/wos/WOS:001176952000001 volume:22 issue:2 firstpage:1 lastpage:16 numberofpages:16 journal:MARINE DRUGS https://hdl.handle.net/11573/1702307 doi:10.3390/md22020061 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85185898327 info:eu-repo/semantics/openAccess staphylococcus epidermidi antibiofilm azurin cold-adapted bacteria info:eu-repo/semantics/article 2024 ftunivromairis https://doi.org/10.3390/md22020061 2024-04-11T00:08:17Z Biofilm is accountable for nosocomial infections and chronic illness, making it a serious economic and public health problem. Staphylococcus epidermidis, thanks to its ability to form biofilm and colonize biomaterials, represents the most frequent causative agent involved in biofilm-associated infections of medical devices. Therefore, the research of new molecules able to interfere with S. epidermidis biofilm formation has a remarkable interest. In the present work, the attention was focused on Pseudomonas sp. TAE6080, an Antarctic marine bacterium able to produce and secrete an effective antibiofilm compound. The molecule responsible for this activity was purified by an activity-guided approach and identified by LC-MS/MS. Results indicated the active protein was a periplasmic protein similar to the Pseudomonas aeruginosa PAO1 azurin, named cold-azurin. The cold-azurin was recombinantly produced in E. coli and purified. The recombinant protein was able to impair S. epidermidis attachment to the polystyrene surface and effectively prevent biofilm formation. Article in Journal/Newspaper Antarc* Antarctic Sapienza Università di Roma: CINECA IRIS Marine Drugs 22 2 61 |
institution |
Open Polar |
collection |
Sapienza Università di Roma: CINECA IRIS |
op_collection_id |
ftunivromairis |
language |
English |
topic |
staphylococcus epidermidi antibiofilm azurin cold-adapted bacteria |
spellingShingle |
staphylococcus epidermidi antibiofilm azurin cold-adapted bacteria Caterina D’Angelo Marika Trecca Andrea Carpentieri Marco Artini Laura Selan Maria Luisa Tutino Rosanna Papa Ermenegilda Parrilli Cold-azurin, a new antibiofilm protein produced by the antarctic marine bacterium pseudomonas sp. tae6080 |
topic_facet |
staphylococcus epidermidi antibiofilm azurin cold-adapted bacteria |
description |
Biofilm is accountable for nosocomial infections and chronic illness, making it a serious economic and public health problem. Staphylococcus epidermidis, thanks to its ability to form biofilm and colonize biomaterials, represents the most frequent causative agent involved in biofilm-associated infections of medical devices. Therefore, the research of new molecules able to interfere with S. epidermidis biofilm formation has a remarkable interest. In the present work, the attention was focused on Pseudomonas sp. TAE6080, an Antarctic marine bacterium able to produce and secrete an effective antibiofilm compound. The molecule responsible for this activity was purified by an activity-guided approach and identified by LC-MS/MS. Results indicated the active protein was a periplasmic protein similar to the Pseudomonas aeruginosa PAO1 azurin, named cold-azurin. The cold-azurin was recombinantly produced in E. coli and purified. The recombinant protein was able to impair S. epidermidis attachment to the polystyrene surface and effectively prevent biofilm formation. |
author2 |
D’Angelo, Caterina Trecca, Marika Carpentieri, Andrea Artini, Marco Selan, Laura Luisa Tutino, Maria Papa, Rosanna Parrilli, Ermenegilda |
format |
Article in Journal/Newspaper |
author |
Caterina D’Angelo Marika Trecca Andrea Carpentieri Marco Artini Laura Selan Maria Luisa Tutino Rosanna Papa Ermenegilda Parrilli |
author_facet |
Caterina D’Angelo Marika Trecca Andrea Carpentieri Marco Artini Laura Selan Maria Luisa Tutino Rosanna Papa Ermenegilda Parrilli |
author_sort |
Caterina D’Angelo |
title |
Cold-azurin, a new antibiofilm protein produced by the antarctic marine bacterium pseudomonas sp. tae6080 |
title_short |
Cold-azurin, a new antibiofilm protein produced by the antarctic marine bacterium pseudomonas sp. tae6080 |
title_full |
Cold-azurin, a new antibiofilm protein produced by the antarctic marine bacterium pseudomonas sp. tae6080 |
title_fullStr |
Cold-azurin, a new antibiofilm protein produced by the antarctic marine bacterium pseudomonas sp. tae6080 |
title_full_unstemmed |
Cold-azurin, a new antibiofilm protein produced by the antarctic marine bacterium pseudomonas sp. tae6080 |
title_sort |
cold-azurin, a new antibiofilm protein produced by the antarctic marine bacterium pseudomonas sp. tae6080 |
publishDate |
2024 |
url |
https://hdl.handle.net/11573/1702307 https://doi.org/10.3390/md22020061 |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/38393032 info:eu-repo/semantics/altIdentifier/wos/WOS:001176952000001 volume:22 issue:2 firstpage:1 lastpage:16 numberofpages:16 journal:MARINE DRUGS https://hdl.handle.net/11573/1702307 doi:10.3390/md22020061 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85185898327 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3390/md22020061 |
container_title |
Marine Drugs |
container_volume |
22 |
container_issue |
2 |
container_start_page |
61 |
_version_ |
1796936164462034944 |