Biological activities of the aerial and undergound parts of Gymnadenia nigra Rchb.f. (syn. Nigritella nigra (L.) Rchb. f.) from the Italian Alps
This study investigated the bioactivity of both aerial (GNAR) and underground (GNUG) parts of Gymnadenia nigra Rchb.f. (syn. Nigritella nigra (L.) Rchb. f.) (Orchidaceae). The obtained data proved interesting when the samples were tested in two adrenocortical cancer cell lines (SW13 and H295R). In p...
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2023
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ftunivromairis:oai:iris.uniroma1.it:11573/1689476 2024-04-14T08:15:11+00:00 Biological activities of the aerial and undergound parts of Gymnadenia nigra Rchb.f. (syn. Nigritella nigra (L.) Rchb. f.) from the Italian Alps Sara Vitalini Beatrice Rubin Halenya Monticelli Susi Barollo Marco Redaelli Loris Bertazza Caterina Mian Maira Zorzan Stefania Garzoli Marcello Iriti Raffaele Pezzani Vitalini, Sara Rubin, Beatrice Monticelli, Halenya Barollo, Susi Redaelli, Marco Bertazza, Lori Mian, Caterina Zorzan, Maira Garzoli, Stefania Iriti, Marcello Pezzani, Raffaele 2023 https://hdl.handle.net/11573/1689476 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:001071066300001 journal:NATURAL PRODUCT RESEARCH https://hdl.handle.net/11573/1689476 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85171658104 Black vanilla orchid antioxidant activity anti-inflammatory activity anti-proliferative activity info:eu-repo/semantics/article 2023 ftunivromairis 2024-03-21T18:23:06Z This study investigated the bioactivity of both aerial (GNAR) and underground (GNUG) parts of Gymnadenia nigra Rchb.f. (syn. Nigritella nigra (L.) Rchb. f.) (Orchidaceae). The obtained data proved interesting when the samples were tested in two adrenocortical cancer cell lines (SW13 and H295R). In particular, the GNAR 80% methanol extract distinctly inhibited their viability after 24 h at a concentration of 1 μg/μL by MTT assay and trypan blue dye exclusion method. Cell morphology evaluation by means Wright’s staining also showed significant results, particularly in SW13 cells under the effect of both extracts. GNAR extract was able to scavenge the DPPH radical better than GNUG extract. It also was more active in albumin denaturation (a maximum % denaturation equal to 463.0 ± 8.3 vs 77.3 ± 13.3) and protease inhibition (a maximum % inhibition equal to 138.5 ± 7.0 vs 2.1 ± 2.0) tests. The results highlighted an important antitumor activity of G. nigra in vitro that deserves to be further studied. Article in Journal/Newspaper Nigritella nigra Sapienza Università di Roma: CINECA IRIS |
institution |
Open Polar |
collection |
Sapienza Università di Roma: CINECA IRIS |
op_collection_id |
ftunivromairis |
language |
English |
topic |
Black vanilla orchid antioxidant activity anti-inflammatory activity anti-proliferative activity |
spellingShingle |
Black vanilla orchid antioxidant activity anti-inflammatory activity anti-proliferative activity Sara Vitalini Beatrice Rubin Halenya Monticelli Susi Barollo Marco Redaelli Loris Bertazza Caterina Mian Maira Zorzan Stefania Garzoli Marcello Iriti Raffaele Pezzani Biological activities of the aerial and undergound parts of Gymnadenia nigra Rchb.f. (syn. Nigritella nigra (L.) Rchb. f.) from the Italian Alps |
topic_facet |
Black vanilla orchid antioxidant activity anti-inflammatory activity anti-proliferative activity |
description |
This study investigated the bioactivity of both aerial (GNAR) and underground (GNUG) parts of Gymnadenia nigra Rchb.f. (syn. Nigritella nigra (L.) Rchb. f.) (Orchidaceae). The obtained data proved interesting when the samples were tested in two adrenocortical cancer cell lines (SW13 and H295R). In particular, the GNAR 80% methanol extract distinctly inhibited their viability after 24 h at a concentration of 1 μg/μL by MTT assay and trypan blue dye exclusion method. Cell morphology evaluation by means Wright’s staining also showed significant results, particularly in SW13 cells under the effect of both extracts. GNAR extract was able to scavenge the DPPH radical better than GNUG extract. It also was more active in albumin denaturation (a maximum % denaturation equal to 463.0 ± 8.3 vs 77.3 ± 13.3) and protease inhibition (a maximum % inhibition equal to 138.5 ± 7.0 vs 2.1 ± 2.0) tests. The results highlighted an important antitumor activity of G. nigra in vitro that deserves to be further studied. |
author2 |
Vitalini, Sara Rubin, Beatrice Monticelli, Halenya Barollo, Susi Redaelli, Marco Bertazza, Lori Mian, Caterina Zorzan, Maira Garzoli, Stefania Iriti, Marcello Pezzani, Raffaele |
format |
Article in Journal/Newspaper |
author |
Sara Vitalini Beatrice Rubin Halenya Monticelli Susi Barollo Marco Redaelli Loris Bertazza Caterina Mian Maira Zorzan Stefania Garzoli Marcello Iriti Raffaele Pezzani |
author_facet |
Sara Vitalini Beatrice Rubin Halenya Monticelli Susi Barollo Marco Redaelli Loris Bertazza Caterina Mian Maira Zorzan Stefania Garzoli Marcello Iriti Raffaele Pezzani |
author_sort |
Sara Vitalini |
title |
Biological activities of the aerial and undergound parts of Gymnadenia nigra Rchb.f. (syn. Nigritella nigra (L.) Rchb. f.) from the Italian Alps |
title_short |
Biological activities of the aerial and undergound parts of Gymnadenia nigra Rchb.f. (syn. Nigritella nigra (L.) Rchb. f.) from the Italian Alps |
title_full |
Biological activities of the aerial and undergound parts of Gymnadenia nigra Rchb.f. (syn. Nigritella nigra (L.) Rchb. f.) from the Italian Alps |
title_fullStr |
Biological activities of the aerial and undergound parts of Gymnadenia nigra Rchb.f. (syn. Nigritella nigra (L.) Rchb. f.) from the Italian Alps |
title_full_unstemmed |
Biological activities of the aerial and undergound parts of Gymnadenia nigra Rchb.f. (syn. Nigritella nigra (L.) Rchb. f.) from the Italian Alps |
title_sort |
biological activities of the aerial and undergound parts of gymnadenia nigra rchb.f. (syn. nigritella nigra (l.) rchb. f.) from the italian alps |
publishDate |
2023 |
url |
https://hdl.handle.net/11573/1689476 |
genre |
Nigritella nigra |
genre_facet |
Nigritella nigra |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:001071066300001 journal:NATURAL PRODUCT RESEARCH https://hdl.handle.net/11573/1689476 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85171658104 |
_version_ |
1796313459914178560 |