Molecular phylogenetics and diagnosis of Anisakis, Pseudoterranova, and Contracaecum from northern pacific marine mammals
Individual specimens of Anisakis, Pseudoterranova, and Contracaecum collected from marine mammals inhabiting northern Pacific waters were used for comparative diagnostic and molecular phylogenetic analyses. Forty-eight new sequences were obtained for this study of 14 Anisakis taxa, 8 Pseudoterranova...
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Format: | Article in Journal/Newspaper |
Language: | English |
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American Society of Parasitologists:Wake Forest University, Biology Department:Winston-Salem, NC 27109:(910)759-5323, Fax: (910)759-6008
2005
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Online Access: | http://hdl.handle.net/11573/127963 https://doi.org/10.1645/ge-522r.1 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000235398400030&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a http://www.scopus.com/inward/record.url?eid=2-s2.0-32544434979&partnerID=65&md5=219911febc323a6fa4c8bb0103d092be |
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ftunivromairis:oai:iris.uniroma1.it:11573/127963 2024-02-11T10:03:30+01:00 Molecular phylogenetics and diagnosis of Anisakis, Pseudoterranova, and Contracaecum from northern pacific marine mammals Steven A. Nadler Murray D. Dailey Sandra Siu Judy A. Sakanari D'AMELIO, Stefano PAGGI, Lia Steven A., Nadler D'Amelio, Stefano Murray D., Dailey Paggi, Lia Sandra, Siu Judy A., Sakanari 2005 http://hdl.handle.net/11573/127963 https://doi.org/10.1645/ge-522r.1 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000235398400030&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a http://www.scopus.com/inward/record.url?eid=2-s2.0-32544434979&partnerID=65&md5=219911febc323a6fa4c8bb0103d092be eng eng American Society of Parasitologists:Wake Forest University, Biology Department:Winston-Salem, NC 27109:(910)759-5323, Fax: (910)759-6008 info:eu-repo/semantics/altIdentifier/pmid/16539026 info:eu-repo/semantics/altIdentifier/wos/WOS:000235398400030 volume:91 issue:6 firstpage:1413 lastpage:1429 journal:THE JOURNAL OF PARASITOLOGY http://hdl.handle.net/11573/127963 doi:10.1645/ge-522r.1 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-32544434979 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000235398400030&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a http://www.scopus.com/inward/record.url?eid=2-s2.0-32544434979&partnerID=65&md5=219911febc323a6fa4c8bb0103d092be info:eu-repo/semantics/article 2005 ftunivromairis https://doi.org/10.1645/ge-522r.1 2024-01-17T17:45:01Z Individual specimens of Anisakis, Pseudoterranova, and Contracaecum collected from marine mammals inhabiting northern Pacific waters were used for comparative diagnostic and molecular phylogenetic analyses. Forty-eight new sequences were obtained for this study of 14 Anisakis taxa, 8 Pseudoterranova taxa, 4 Contracaecum taxa, and 4 outgroup species. Partial 28S (LSU) and complete internal transcribed spacer (ITS-1, 5.8S, ITS-2) ribosomal DNA was amplified by the polymerase chain reaction and sequenced. Sequences of ITS indicated that Pseudoterranova specimens from Zalophus californianus (California sea lion), Mirounga angustirostris (northern elephant seal), Phoca vitulina (harbor seal), Enhydra lutris (sea otter), and Eumetopias jubatus (Steller's sea lion) exactly matched P. decipiens s. Str., extending the host and geographic range of this species. Anisakis from northern Pacific marine mammals were most closely related to members of the A. simplex species complex. Comparison of Anisakis ITS sequences diagnosed the presence of A. simplex C in 2 M. angustirostris hosts, which is a new host record. Anisakis specimens from Phocoena phocoena (harbor porpoise), Lissodelphis borealis (Pacific rightwhale porpoise), and E. jubatus included 3 ITS sequences that did not match any known species. Contracaecum adults obtained from Z. californianus were most closely related to C. ogmorhini s.l. and C. rudolphii, but ITS sequences of these Contracaecum specimens did not match C. ogmorhini s. str. or C. margolisi. These novel Anisakis and Contracaecum ITS sequences may represent previously uncharacterized species. Phylogenetic analysis of LSU sequences revealed strong support for the monophyly of Anisakinae, Contracaecum plus Phocascaris, Pseudoterranova, and Anisakis. Phylogenetic trees inferred from ITS sequences yielded robustly supported relationships for Pseudoterranova and Anisakis species that are primarily consistent with previously published phenograms based on multilocus electrophoretic data. © American Society of ... Article in Journal/Newspaper Elephant Seal harbor seal Phoca vitulina Phocoena phocoena Sapienza Università di Roma: CINECA IRIS Pacific Journal of Parasitology 91 6 1413 1429 |
institution |
Open Polar |
collection |
Sapienza Università di Roma: CINECA IRIS |
op_collection_id |
ftunivromairis |
language |
English |
description |
Individual specimens of Anisakis, Pseudoterranova, and Contracaecum collected from marine mammals inhabiting northern Pacific waters were used for comparative diagnostic and molecular phylogenetic analyses. Forty-eight new sequences were obtained for this study of 14 Anisakis taxa, 8 Pseudoterranova taxa, 4 Contracaecum taxa, and 4 outgroup species. Partial 28S (LSU) and complete internal transcribed spacer (ITS-1, 5.8S, ITS-2) ribosomal DNA was amplified by the polymerase chain reaction and sequenced. Sequences of ITS indicated that Pseudoterranova specimens from Zalophus californianus (California sea lion), Mirounga angustirostris (northern elephant seal), Phoca vitulina (harbor seal), Enhydra lutris (sea otter), and Eumetopias jubatus (Steller's sea lion) exactly matched P. decipiens s. Str., extending the host and geographic range of this species. Anisakis from northern Pacific marine mammals were most closely related to members of the A. simplex species complex. Comparison of Anisakis ITS sequences diagnosed the presence of A. simplex C in 2 M. angustirostris hosts, which is a new host record. Anisakis specimens from Phocoena phocoena (harbor porpoise), Lissodelphis borealis (Pacific rightwhale porpoise), and E. jubatus included 3 ITS sequences that did not match any known species. Contracaecum adults obtained from Z. californianus were most closely related to C. ogmorhini s.l. and C. rudolphii, but ITS sequences of these Contracaecum specimens did not match C. ogmorhini s. str. or C. margolisi. These novel Anisakis and Contracaecum ITS sequences may represent previously uncharacterized species. Phylogenetic analysis of LSU sequences revealed strong support for the monophyly of Anisakinae, Contracaecum plus Phocascaris, Pseudoterranova, and Anisakis. Phylogenetic trees inferred from ITS sequences yielded robustly supported relationships for Pseudoterranova and Anisakis species that are primarily consistent with previously published phenograms based on multilocus electrophoretic data. © American Society of ... |
author2 |
Steven A., Nadler D'Amelio, Stefano Murray D., Dailey Paggi, Lia Sandra, Siu Judy A., Sakanari |
format |
Article in Journal/Newspaper |
author |
Steven A. Nadler Murray D. Dailey Sandra Siu Judy A. Sakanari D'AMELIO, Stefano PAGGI, Lia |
spellingShingle |
Steven A. Nadler Murray D. Dailey Sandra Siu Judy A. Sakanari D'AMELIO, Stefano PAGGI, Lia Molecular phylogenetics and diagnosis of Anisakis, Pseudoterranova, and Contracaecum from northern pacific marine mammals |
author_facet |
Steven A. Nadler Murray D. Dailey Sandra Siu Judy A. Sakanari D'AMELIO, Stefano PAGGI, Lia |
author_sort |
Steven A. Nadler |
title |
Molecular phylogenetics and diagnosis of Anisakis, Pseudoterranova, and Contracaecum from northern pacific marine mammals |
title_short |
Molecular phylogenetics and diagnosis of Anisakis, Pseudoterranova, and Contracaecum from northern pacific marine mammals |
title_full |
Molecular phylogenetics and diagnosis of Anisakis, Pseudoterranova, and Contracaecum from northern pacific marine mammals |
title_fullStr |
Molecular phylogenetics and diagnosis of Anisakis, Pseudoterranova, and Contracaecum from northern pacific marine mammals |
title_full_unstemmed |
Molecular phylogenetics and diagnosis of Anisakis, Pseudoterranova, and Contracaecum from northern pacific marine mammals |
title_sort |
molecular phylogenetics and diagnosis of anisakis, pseudoterranova, and contracaecum from northern pacific marine mammals |
publisher |
American Society of Parasitologists:Wake Forest University, Biology Department:Winston-Salem, NC 27109:(910)759-5323, Fax: (910)759-6008 |
publishDate |
2005 |
url |
http://hdl.handle.net/11573/127963 https://doi.org/10.1645/ge-522r.1 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000235398400030&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a http://www.scopus.com/inward/record.url?eid=2-s2.0-32544434979&partnerID=65&md5=219911febc323a6fa4c8bb0103d092be |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Elephant Seal harbor seal Phoca vitulina Phocoena phocoena |
genre_facet |
Elephant Seal harbor seal Phoca vitulina Phocoena phocoena |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/16539026 info:eu-repo/semantics/altIdentifier/wos/WOS:000235398400030 volume:91 issue:6 firstpage:1413 lastpage:1429 journal:THE JOURNAL OF PARASITOLOGY http://hdl.handle.net/11573/127963 doi:10.1645/ge-522r.1 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-32544434979 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000235398400030&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a http://www.scopus.com/inward/record.url?eid=2-s2.0-32544434979&partnerID=65&md5=219911febc323a6fa4c8bb0103d092be |
op_doi |
https://doi.org/10.1645/ge-522r.1 |
container_title |
Journal of Parasitology |
container_volume |
91 |
container_issue |
6 |
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1413 |
op_container_end_page |
1429 |
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1790599758846361600 |