SSCP-based identification of members within the Pseudoterranova decipiens complex (Nematoda : Ascaridoidea : Anisakidae) using genetic markers in the internal transcribed spacers of ribosomal DNA
The anisakid nematodes morphologically corresponding with Pseudoterranova decipiens sensu lato (s.l.) (Krabbe, 1878) from different seal or sea lion hosts and geographical origins, previously identified as Pseudoterranova krabbei, P. decipiens (s.s.), P. bulbosa, P. azarasi and P. cattani by multilo...
Published in: | Parasitology |
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Language: | English |
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CAMBRIDGE UNIV PRESS
2002
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Online Access: | http://hdl.handle.net/11573/127088 https://doi.org/10.1017/s0031182002001579 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000177212400006&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a |
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ftunivromairis:oai:iris.uniroma1.it:11573/127088 2024-02-27T08:35:17+00:00 SSCP-based identification of members within the Pseudoterranova decipiens complex (Nematoda : Ascaridoidea : Anisakidae) using genetic markers in the internal transcribed spacers of ribosomal DNA X. q Zhu H. w Palm M. George Nascimento R. b Gasser D'AMELIO, Stefano PAGGI, Lia X., q Zhu D'Amelio, Stefano H., w Palm Paggi, Lia M., George Nascimento R., b Gasser 2002 http://hdl.handle.net/11573/127088 https://doi.org/10.1017/s0031182002001579 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000177212400006&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a eng eng CAMBRIDGE UNIV PRESS info:eu-repo/semantics/altIdentifier/pmid/12118717 info:eu-repo/semantics/altIdentifier/wos/WOS:000177212400006 volume:124 issue:Pt 6 firstpage:615 lastpage:623 numberofpages:9 journal:PARASITOLOGY http://hdl.handle.net/11573/127088 doi:10.1017/s0031182002001579 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0036308095 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000177212400006&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a internal transcribed spacer polymerase chain reaction pseudoterranova decipiens sensu lato ribosomal dna single-strand conformation polymorphism species identification info:eu-repo/semantics/article 2002 ftunivromairis https://doi.org/10.1017/s0031182002001579 2024-01-31T17:45:48Z The anisakid nematodes morphologically corresponding with Pseudoterranova decipiens sensu lato (s.l.) (Krabbe, 1878) from different seal or sea lion hosts and geographical origins, previously identified as Pseudoterranova krabbei, P. decipiens (s.s.), P. bulbosa, P. azarasi and P. cattani by multilocus enzyme electrophoresis, were characterized using a DNA approach. Also a population of P. decipiens (s.l.) from Chaenocephalus aceratus, the blackfin icefish, from Antarctica and another from Osmerus eperlanus, the European smelt, from Germany were included in the study. The first (ITS-1) and second (ITS-2) internal transcribed spacers (ITS) of ribosomal DNA (rDNA) were amplified by PCR from individual nematodes and analysed by single-strand conformation polymorphism (SSCP), followed by selective sequencing. While no variation in single-stranded ITS-1 and ITS-2 profiles was detected among samples representing each of the species or populations (with the exception of slight microheterogeneity), SSCP analysis of the ITS-2 amplicons allowed the unequivocal differentiation of all of the 5 sibling species of P. decipiens (s.l.) examined, which was supported by sequence differences in ITS rDNA. Samples representing the P. decipiens (s.l.) population from O. eperlanus had the same SSCP profile as those of P. decipiens (s.s.), which was supported by a lack of nucleotide difference in the ITS between them, suggesting that the former represented P. decipiens (s.s.). Based on SSCP results and ITS sequence data, P. decipiens (s.l.) from C. aceratus was genetically most distinct with respect to all other members of Pseudoterranova examined, which indicated that it may represent P. decipiens E (based on geographical origin) or a distinct species. These findings and the molecular approach taken should have important implications for studying the life-cycles, transmission patterns, epidemiology and population genetics of these anisakid nematodes, and the diagnosis of their infections. Article in Journal/Newspaper Antarc* Antarctica Icefish Sapienza Università di Roma: CINECA IRIS Parasitology 124 6 615 623 |
institution |
Open Polar |
collection |
Sapienza Università di Roma: CINECA IRIS |
op_collection_id |
ftunivromairis |
language |
English |
topic |
internal transcribed spacer polymerase chain reaction pseudoterranova decipiens sensu lato ribosomal dna single-strand conformation polymorphism species identification |
spellingShingle |
internal transcribed spacer polymerase chain reaction pseudoterranova decipiens sensu lato ribosomal dna single-strand conformation polymorphism species identification X. q Zhu H. w Palm M. George Nascimento R. b Gasser D'AMELIO, Stefano PAGGI, Lia SSCP-based identification of members within the Pseudoterranova decipiens complex (Nematoda : Ascaridoidea : Anisakidae) using genetic markers in the internal transcribed spacers of ribosomal DNA |
topic_facet |
internal transcribed spacer polymerase chain reaction pseudoterranova decipiens sensu lato ribosomal dna single-strand conformation polymorphism species identification |
description |
The anisakid nematodes morphologically corresponding with Pseudoterranova decipiens sensu lato (s.l.) (Krabbe, 1878) from different seal or sea lion hosts and geographical origins, previously identified as Pseudoterranova krabbei, P. decipiens (s.s.), P. bulbosa, P. azarasi and P. cattani by multilocus enzyme electrophoresis, were characterized using a DNA approach. Also a population of P. decipiens (s.l.) from Chaenocephalus aceratus, the blackfin icefish, from Antarctica and another from Osmerus eperlanus, the European smelt, from Germany were included in the study. The first (ITS-1) and second (ITS-2) internal transcribed spacers (ITS) of ribosomal DNA (rDNA) were amplified by PCR from individual nematodes and analysed by single-strand conformation polymorphism (SSCP), followed by selective sequencing. While no variation in single-stranded ITS-1 and ITS-2 profiles was detected among samples representing each of the species or populations (with the exception of slight microheterogeneity), SSCP analysis of the ITS-2 amplicons allowed the unequivocal differentiation of all of the 5 sibling species of P. decipiens (s.l.) examined, which was supported by sequence differences in ITS rDNA. Samples representing the P. decipiens (s.l.) population from O. eperlanus had the same SSCP profile as those of P. decipiens (s.s.), which was supported by a lack of nucleotide difference in the ITS between them, suggesting that the former represented P. decipiens (s.s.). Based on SSCP results and ITS sequence data, P. decipiens (s.l.) from C. aceratus was genetically most distinct with respect to all other members of Pseudoterranova examined, which indicated that it may represent P. decipiens E (based on geographical origin) or a distinct species. These findings and the molecular approach taken should have important implications for studying the life-cycles, transmission patterns, epidemiology and population genetics of these anisakid nematodes, and the diagnosis of their infections. |
author2 |
X., q Zhu D'Amelio, Stefano H., w Palm Paggi, Lia M., George Nascimento R., b Gasser |
format |
Article in Journal/Newspaper |
author |
X. q Zhu H. w Palm M. George Nascimento R. b Gasser D'AMELIO, Stefano PAGGI, Lia |
author_facet |
X. q Zhu H. w Palm M. George Nascimento R. b Gasser D'AMELIO, Stefano PAGGI, Lia |
author_sort |
X. q Zhu |
title |
SSCP-based identification of members within the Pseudoterranova decipiens complex (Nematoda : Ascaridoidea : Anisakidae) using genetic markers in the internal transcribed spacers of ribosomal DNA |
title_short |
SSCP-based identification of members within the Pseudoterranova decipiens complex (Nematoda : Ascaridoidea : Anisakidae) using genetic markers in the internal transcribed spacers of ribosomal DNA |
title_full |
SSCP-based identification of members within the Pseudoterranova decipiens complex (Nematoda : Ascaridoidea : Anisakidae) using genetic markers in the internal transcribed spacers of ribosomal DNA |
title_fullStr |
SSCP-based identification of members within the Pseudoterranova decipiens complex (Nematoda : Ascaridoidea : Anisakidae) using genetic markers in the internal transcribed spacers of ribosomal DNA |
title_full_unstemmed |
SSCP-based identification of members within the Pseudoterranova decipiens complex (Nematoda : Ascaridoidea : Anisakidae) using genetic markers in the internal transcribed spacers of ribosomal DNA |
title_sort |
sscp-based identification of members within the pseudoterranova decipiens complex (nematoda : ascaridoidea : anisakidae) using genetic markers in the internal transcribed spacers of ribosomal dna |
publisher |
CAMBRIDGE UNIV PRESS |
publishDate |
2002 |
url |
http://hdl.handle.net/11573/127088 https://doi.org/10.1017/s0031182002001579 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000177212400006&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a |
genre |
Antarc* Antarctica Icefish |
genre_facet |
Antarc* Antarctica Icefish |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/12118717 info:eu-repo/semantics/altIdentifier/wos/WOS:000177212400006 volume:124 issue:Pt 6 firstpage:615 lastpage:623 numberofpages:9 journal:PARASITOLOGY http://hdl.handle.net/11573/127088 doi:10.1017/s0031182002001579 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0036308095 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000177212400006&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a |
op_doi |
https://doi.org/10.1017/s0031182002001579 |
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Parasitology |
container_volume |
124 |
container_issue |
6 |
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615 |
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623 |
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