Production and characterization of lipopeptide biosurfactant from a new strain of Pseudomonas antarctica 28E using crude glycerol as a carbon source
Pseudomonas is a cosmopolitan genus of bacteria found in soil, water, organic matter, plants and animals and known for the production of glycolipid and lipopeptide biosurfactants. In this study bacteria (laboratory collection number 28E) isolated from soil collected in Spitsbergen were used for bios...
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Online Access: | https://hdl.handle.net/11590/450948 https://doi.org/10.1039/d3ra03408a |
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ftunivroma3iris:oai:iris.uniroma3.it:11590/450948 2024-02-27T08:33:25+00:00 Production and characterization of lipopeptide biosurfactant from a new strain of Pseudomonas antarctica 28E using crude glycerol as a carbon source Ciurko, Dominika Chebbi, Alif Kruszelnicki, Mateusz Czapor-Irzabek, Hanna Urbanek, Aneta K Polowczyk, Izabela Franzetti, Andrea Janek, Tomasz Ciurko, Dominika Chebbi, Alif Kruszelnicki, Mateusz Czapor-Irzabek, Hanna Urbanek, Aneta K Polowczyk, Izabela Franzetti, Andrea Janek, Tomasz 2023 https://hdl.handle.net/11590/450948 https://doi.org/10.1039/d3ra03408a eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:001045778600001 volume:13 issue:34 firstpage:24129 lastpage:24139 numberofpages:11 journal:RSC ADVANCES https://hdl.handle.net/11590/450948 doi:10.1039/d3ra03408a info:eu-repo/semantics/article 2023 ftunivroma3iris https://doi.org/10.1039/d3ra03408a 2024-01-31T17:33:27Z Pseudomonas is a cosmopolitan genus of bacteria found in soil, water, organic matter, plants and animals and known for the production of glycolipid and lipopeptide biosurfactants. In this study bacteria (laboratory collection number 28E) isolated from soil collected in Spitsbergen were used for biosurfactant production. 16S rRNA sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) revealed that this isolate belongs to the species Pseudomonas antarctica. In the present study, crude glycerol, a raw material obtained from several industrial processes, was evaluated as a potential low-cost carbon source to reduce the costs of lipopeptide production. Among several tested glycerols, a waste product of stearin production, rich in nitrogen, iron and calcium, ensured optimal conditions for bacterial growth. Biosurfactant production was evidenced by a reduction of surface tension (ST) and an increase in the emulsification index (E-24%). According to Fourier-transform infrared spectroscopy (FTIR) and electrospray ionization mass spectrometry (ESI-MS), the biosurfactant was identified as viscosin. The critical micelle concentration (CMC) of lipopeptide was determined to be 20 mg L-1. Interestingly, viscosin production has been reported previously for Pseudomonas viscosa, Pseudomonas fluorescens and Pseudomonas libanensis. To the best of our knowledge, this is the first report on viscosin production by a P. antarctica 28E. The results indicated the potential of crude glycerol as a low-cost substrate to produce a lipopeptide biosurfactant with promising tensioactive and emulsifying properties. Article in Journal/Newspaper Antarc* Antarctica Spitsbergen Anagrafe della Ricerca d'Ateneo (Universitá degli studi Roma Tre) RSC Advances 13 34 24129 24139 |
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Open Polar |
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Anagrafe della Ricerca d'Ateneo (Universitá degli studi Roma Tre) |
op_collection_id |
ftunivroma3iris |
language |
English |
description |
Pseudomonas is a cosmopolitan genus of bacteria found in soil, water, organic matter, plants and animals and known for the production of glycolipid and lipopeptide biosurfactants. In this study bacteria (laboratory collection number 28E) isolated from soil collected in Spitsbergen were used for biosurfactant production. 16S rRNA sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) revealed that this isolate belongs to the species Pseudomonas antarctica. In the present study, crude glycerol, a raw material obtained from several industrial processes, was evaluated as a potential low-cost carbon source to reduce the costs of lipopeptide production. Among several tested glycerols, a waste product of stearin production, rich in nitrogen, iron and calcium, ensured optimal conditions for bacterial growth. Biosurfactant production was evidenced by a reduction of surface tension (ST) and an increase in the emulsification index (E-24%). According to Fourier-transform infrared spectroscopy (FTIR) and electrospray ionization mass spectrometry (ESI-MS), the biosurfactant was identified as viscosin. The critical micelle concentration (CMC) of lipopeptide was determined to be 20 mg L-1. Interestingly, viscosin production has been reported previously for Pseudomonas viscosa, Pseudomonas fluorescens and Pseudomonas libanensis. To the best of our knowledge, this is the first report on viscosin production by a P. antarctica 28E. The results indicated the potential of crude glycerol as a low-cost substrate to produce a lipopeptide biosurfactant with promising tensioactive and emulsifying properties. |
author2 |
Ciurko, Dominika Chebbi, Alif Kruszelnicki, Mateusz Czapor-Irzabek, Hanna Urbanek, Aneta K Polowczyk, Izabela Franzetti, Andrea Janek, Tomasz |
format |
Article in Journal/Newspaper |
author |
Ciurko, Dominika Chebbi, Alif Kruszelnicki, Mateusz Czapor-Irzabek, Hanna Urbanek, Aneta K Polowczyk, Izabela Franzetti, Andrea Janek, Tomasz |
spellingShingle |
Ciurko, Dominika Chebbi, Alif Kruszelnicki, Mateusz Czapor-Irzabek, Hanna Urbanek, Aneta K Polowczyk, Izabela Franzetti, Andrea Janek, Tomasz Production and characterization of lipopeptide biosurfactant from a new strain of Pseudomonas antarctica 28E using crude glycerol as a carbon source |
author_facet |
Ciurko, Dominika Chebbi, Alif Kruszelnicki, Mateusz Czapor-Irzabek, Hanna Urbanek, Aneta K Polowczyk, Izabela Franzetti, Andrea Janek, Tomasz |
author_sort |
Ciurko, Dominika |
title |
Production and characterization of lipopeptide biosurfactant from a new strain of Pseudomonas antarctica 28E using crude glycerol as a carbon source |
title_short |
Production and characterization of lipopeptide biosurfactant from a new strain of Pseudomonas antarctica 28E using crude glycerol as a carbon source |
title_full |
Production and characterization of lipopeptide biosurfactant from a new strain of Pseudomonas antarctica 28E using crude glycerol as a carbon source |
title_fullStr |
Production and characterization of lipopeptide biosurfactant from a new strain of Pseudomonas antarctica 28E using crude glycerol as a carbon source |
title_full_unstemmed |
Production and characterization of lipopeptide biosurfactant from a new strain of Pseudomonas antarctica 28E using crude glycerol as a carbon source |
title_sort |
production and characterization of lipopeptide biosurfactant from a new strain of pseudomonas antarctica 28e using crude glycerol as a carbon source |
publishDate |
2023 |
url |
https://hdl.handle.net/11590/450948 https://doi.org/10.1039/d3ra03408a |
genre |
Antarc* Antarctica Spitsbergen |
genre_facet |
Antarc* Antarctica Spitsbergen |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:001045778600001 volume:13 issue:34 firstpage:24129 lastpage:24139 numberofpages:11 journal:RSC ADVANCES https://hdl.handle.net/11590/450948 doi:10.1039/d3ra03408a |
op_doi |
https://doi.org/10.1039/d3ra03408a |
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RSC Advances |
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13 |
container_issue |
34 |
container_start_page |
24129 |
op_container_end_page |
24139 |
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