A colostrum trypsin inhibitor gene expressed in the Cape fur seal mammary gland during lactation
International audience The colostrum trypsin inhibitor (CTI) gene and transcript were cloned from the Cape fur seal mammary gland and CTI identified by in silico analysis of the Pacific walrus and polar bear genomes (Order Carnivora), and in marine and terrestrial mammals of the Orders Cetartiodacty...
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Online Access: | https://hal.science/hal-01294536 https://doi.org/10.1016/j.gene.2015.11.042 |
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ftunivrochelle:oai:HAL:hal-01294536v1 2024-02-11T10:09:22+01:00 A colostrum trypsin inhibitor gene expressed in the Cape fur seal mammary gland during lactation Pharo, Elizabeth A. Cane, Kylie N. Mccoey, Julia Buckle, Ashley M. Oosthuizen, W. H. Guinet, Christophe Arnould, John P. Y. School of BioSciences Melbourne Faculty of Science Melbourne University of Melbourne-University of Melbourne Cooperative Research Centre for Innovative Dairy Products Monash University Clayton Oceans and Coasts Research Department of Environmental Affairs Centre d'Études Biologiques de Chizé - UMR 7372 (CEBC) Institut National de la Recherche Agronomique (INRA)-La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS) School of Life and Environmental Sciences Deakin University Burwood 2016 https://hal.science/hal-01294536 https://doi.org/10.1016/j.gene.2015.11.042 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.gene.2015.11.042 hal-01294536 https://hal.science/hal-01294536 doi:10.1016/j.gene.2015.11.042 ISSN: 0378-1119 EISSN: 1879-0038 Gene https://hal.science/hal-01294536 Gene, 2016, 578, pp.7-16. ⟨10.1016/j.gene.2015.11.042⟩ Pinniped Kunitz BPTI Protease Walrus Milk [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2016 ftunivrochelle https://doi.org/10.1016/j.gene.2015.11.042 2024-01-23T23:35:46Z International audience The colostrum trypsin inhibitor (CTI) gene and transcript were cloned from the Cape fur seal mammary gland and CTI identified by in silico analysis of the Pacific walrus and polar bear genomes (Order Carnivora), and in marine and terrestrial mammals of the Orders Cetartiodactyla (yak, whales, camel) and Perissodactyla (white rhinoceros). Unexpectedly, Weddell seal CTI was predicted to be a pseudogene. Cape fur seal CTI was expressed in the mammary gland of a pregnant multiparous seal, but not in a seal in its first pregnancy. While bovine CTI is expressed for 24–48 h postpartum (pp) and secreted in colostrum only, Cape fur seal CTI was detected for at least 2–3 months pp while the mother was suckling its young on-shore. Furthermore, CTI was expressed in the mammary gland of only one of the lactating seals that was foraging at-sea. The expression of β-casein (CSN2) and β-lactoglobulin II (LGB2), but not CTI in the second lactating seal foraging at-sea suggested that CTI may be intermittently expressed during lactation. Cape fur seal and walrus CTI encode putative small, secreted, N-glycosylated proteins with a single Kunitz/bovine pancreatic trypsin inhibitor (BPTI) domain indicative of serine protease inhibition. Mature Cape fur seal CTI shares 92% sequence identity with Pacific walrus CTI, but only 35% identity with BPTI. Structural homology modelling of Cape fur seal CTI and Pacific walrus trypsin based on the model of the second Kunitz domain of human tissue factor pathway inhibitor (TFPI) and porcine trypsin (Protein Data Bank: 1TFX) confirmed that CTI inhibits trypsin in a canonical fashion. Therefore, pinniped CTI may be critical for preventing the proteolytic degradation of immunoglobulins that are passively transferred from mother to young via colostrum and milk. Article in Journal/Newspaper Weddell Seal walrus* HAL - Université de La Rochelle Pacific Weddell Gene 578 1 7 16 |
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Open Polar |
collection |
HAL - Université de La Rochelle |
op_collection_id |
ftunivrochelle |
language |
English |
topic |
Pinniped Kunitz BPTI Protease Walrus Milk [SDE]Environmental Sciences |
spellingShingle |
Pinniped Kunitz BPTI Protease Walrus Milk [SDE]Environmental Sciences Pharo, Elizabeth A. Cane, Kylie N. Mccoey, Julia Buckle, Ashley M. Oosthuizen, W. H. Guinet, Christophe Arnould, John P. Y. A colostrum trypsin inhibitor gene expressed in the Cape fur seal mammary gland during lactation |
topic_facet |
Pinniped Kunitz BPTI Protease Walrus Milk [SDE]Environmental Sciences |
description |
International audience The colostrum trypsin inhibitor (CTI) gene and transcript were cloned from the Cape fur seal mammary gland and CTI identified by in silico analysis of the Pacific walrus and polar bear genomes (Order Carnivora), and in marine and terrestrial mammals of the Orders Cetartiodactyla (yak, whales, camel) and Perissodactyla (white rhinoceros). Unexpectedly, Weddell seal CTI was predicted to be a pseudogene. Cape fur seal CTI was expressed in the mammary gland of a pregnant multiparous seal, but not in a seal in its first pregnancy. While bovine CTI is expressed for 24–48 h postpartum (pp) and secreted in colostrum only, Cape fur seal CTI was detected for at least 2–3 months pp while the mother was suckling its young on-shore. Furthermore, CTI was expressed in the mammary gland of only one of the lactating seals that was foraging at-sea. The expression of β-casein (CSN2) and β-lactoglobulin II (LGB2), but not CTI in the second lactating seal foraging at-sea suggested that CTI may be intermittently expressed during lactation. Cape fur seal and walrus CTI encode putative small, secreted, N-glycosylated proteins with a single Kunitz/bovine pancreatic trypsin inhibitor (BPTI) domain indicative of serine protease inhibition. Mature Cape fur seal CTI shares 92% sequence identity with Pacific walrus CTI, but only 35% identity with BPTI. Structural homology modelling of Cape fur seal CTI and Pacific walrus trypsin based on the model of the second Kunitz domain of human tissue factor pathway inhibitor (TFPI) and porcine trypsin (Protein Data Bank: 1TFX) confirmed that CTI inhibits trypsin in a canonical fashion. Therefore, pinniped CTI may be critical for preventing the proteolytic degradation of immunoglobulins that are passively transferred from mother to young via colostrum and milk. |
author2 |
School of BioSciences Melbourne Faculty of Science Melbourne University of Melbourne-University of Melbourne Cooperative Research Centre for Innovative Dairy Products Monash University Clayton Oceans and Coasts Research Department of Environmental Affairs Centre d'Études Biologiques de Chizé - UMR 7372 (CEBC) Institut National de la Recherche Agronomique (INRA)-La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS) School of Life and Environmental Sciences Deakin University Burwood |
format |
Article in Journal/Newspaper |
author |
Pharo, Elizabeth A. Cane, Kylie N. Mccoey, Julia Buckle, Ashley M. Oosthuizen, W. H. Guinet, Christophe Arnould, John P. Y. |
author_facet |
Pharo, Elizabeth A. Cane, Kylie N. Mccoey, Julia Buckle, Ashley M. Oosthuizen, W. H. Guinet, Christophe Arnould, John P. Y. |
author_sort |
Pharo, Elizabeth A. |
title |
A colostrum trypsin inhibitor gene expressed in the Cape fur seal mammary gland during lactation |
title_short |
A colostrum trypsin inhibitor gene expressed in the Cape fur seal mammary gland during lactation |
title_full |
A colostrum trypsin inhibitor gene expressed in the Cape fur seal mammary gland during lactation |
title_fullStr |
A colostrum trypsin inhibitor gene expressed in the Cape fur seal mammary gland during lactation |
title_full_unstemmed |
A colostrum trypsin inhibitor gene expressed in the Cape fur seal mammary gland during lactation |
title_sort |
colostrum trypsin inhibitor gene expressed in the cape fur seal mammary gland during lactation |
publisher |
HAL CCSD |
publishDate |
2016 |
url |
https://hal.science/hal-01294536 https://doi.org/10.1016/j.gene.2015.11.042 |
geographic |
Pacific Weddell |
geographic_facet |
Pacific Weddell |
genre |
Weddell Seal walrus* |
genre_facet |
Weddell Seal walrus* |
op_source |
ISSN: 0378-1119 EISSN: 1879-0038 Gene https://hal.science/hal-01294536 Gene, 2016, 578, pp.7-16. ⟨10.1016/j.gene.2015.11.042⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.gene.2015.11.042 hal-01294536 https://hal.science/hal-01294536 doi:10.1016/j.gene.2015.11.042 |
op_doi |
https://doi.org/10.1016/j.gene.2015.11.042 |
container_title |
Gene |
container_volume |
578 |
container_issue |
1 |
container_start_page |
7 |
op_container_end_page |
16 |
_version_ |
1790609256669511680 |