Detection of early effects of a single herbicide (diuron) and a mix of herbicides and pharmaceuticals (diuron, isoproturon, ibuprofen) on immunological parameters of Pacific oyster (Crassostrea gigas) spat

International audience In the context of massive summer mortality events of the Pacific oyster Crassostrea gigas, the aim of this study was to investigate the early effects on genes, enzymes and haemocyte parameters implicated in immune defence mechanisms in C. gigas oysters exposed to a potentially...

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Bibliographic Details
Published in:Chemosphere
Main Authors: Luna-Acosta, Andrea, Renault, Tristan, Thomas-Guyon, Hélène, Faury, Nicole, Saulnier, Denis, Budzinski, Hélène, Le Ménach, Karine, Pardon, P., Fruitier-Arnaudin, Ingrid, Bustamante, Paco
Other Authors: LIttoral ENvironnement et Sociétés (LIENSs), La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS), Fédération de Recherche en Environnement pour le Développement Durable - FR 3097 (FREDD), Unité Amélioration génétique, Santé animale et Environnement (AGSAE), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Laboratoire de Physico -& Toxico Chimie des systèmes naturels (LPTC), Université Sciences et Technologies - Bordeaux 1 (UB)-Centre National de la Recherche Scientifique (CNRS)
Format: Article in Journal/Newspaper
Language:English
Published: HAL CCSD 2012
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Online Access:https://hal.science/hal-00686855
https://hal.science/hal-00686855/document
https://hal.science/hal-00686855/file/Luna-Acosta_et_al_2012_CHEM.pdf
https://doi.org/10.1016/j.chemosphere.2012.02.022
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Summary:International audience In the context of massive summer mortality events of the Pacific oyster Crassostrea gigas, the aim of this study was to investigate the early effects on genes, enzymes and haemocyte parameters implicated in immune defence mechanisms in C. gigas oysters exposed to a potentially hostile environment, i.e. to an herbicide alone or within a mixture. Following 2 hours of exposure to the herbicide diuron at 1 µg L-1, the repression of different genes implicated in immune defence mechanisms in the haemocytes and the inhibition of enzyme activities, such as laccase-type phenoloxidase (PO) in the plasma, were observed. The inhibition of superoxide dismutase (SOD) activity in the plasma was also observed after 6 and 24 hours of exposure. In the mixture with the herbicides diuron and isoproturon, and the pharmaceutical ibuprofen, catecholase-type PO activity in the plasma and the percentage of phagocytosis in the haemocytes were reduced after 6 hours of exposure. Our results showed that early effects on molecular, biochemical and cellular parameters can be detected in the presence of diuron alone or within a mixture, giving an insight of its potential effect in situations that can be found in natural environments, i.e. relatively high concentrations for short periods of time.