Cytochrome P450 1A1 expression in cetacean skin biopsies from the Indian Ocean

• Published in: Marine Pollution Bulletin
• Main Authors: Jauniaux, Thierry, Farnir, Frédéric, Fontaine, Michaël, Kiszka, Jeremy, Sarlet, Michael, Coignoul, Freddy
• Other Authors: Faculté de Médecine Vétérinaire Liège, Département de Biostatistiques, Université de Liège, Ecologie Systématique et Evolution (ESE), Université Paris-Sud - Paris 11 (UP11)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS), LIttoral ENvironnement et Sociétés (LIENSs), La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS), Département de Pathologie Générale, Faculté de médecine vétérinaire, Département de Pathotologie Générale
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2011
• Subjects: Cytochrome P450; Biopsy; Skin; Immunohistochemistry; Mayotte; PORPOISES PHOCOENA-PHOCOENA; BOTTLE-NOSED-DOLPHIN; TURSIOPS-TRUNCATUS; INTEGUMENT; MARINE MAMMALS; CONTAMINANTS; POLLUTANTS; INDUCTION; EXPOSURE; [SDV.EE.ECO]Life Sciences [q-bio]/Ecology; environment/Ecosystems; Indian; Megaptera novaeangliae; Phocoena phocoena
• Online Access: https://hal.science/hal-00608678; https://hal.science/hal-00608678/document; https://hal.science/hal-00608678/file/CytochromeLast.pdf; https://doi.org/10.1016/j.marpolbul.2011.04.005

International audience The study describes cytochrome P-450 1A1 (CYPA1) expression in the skin of different cetacean species (Megaptera novaeangliae, n= 15; Stenella attenuata, n=7 and Stenella longirostris, n= 24) from the Mozambique Channel island of Mayotte. Immunohistochemical examination was performed with a monoclonal antibody against scup cytochrome CYPA1. The sex was determined using a molecular approach consisting in the genotyping sex-specific genes. CYPA1 was detected at the junction between epidermis and blubber on dolphins only, mostly in the endothelial cells. Similar observation was obtained in the dermis of one M. novaeangliae. Immunohistochemical slides were scored to evaluate the expression of the CYPA1 and a higher expression was observed in S. longirostris, suggesting a higher exposure to pollutants for this species. The difference of expression between sexes was not significant.