Water plays a different role on activation thermodynamic parameters of alcoholysis reaction catalyzed by Lipase in gaseous and organic media
International audience The effect of water on the alcoholysis of methyl propionate and n-propanol catalyzed by immobilized Candida antarctica lipase B (CALB) has been compared in a continuous solid/gas reactor and in an organic liquid medium. The enthalpic and entropic contributions of water to the...
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ftunivrochelle:oai:HAL:hal-00329663v1 2024-02-11T09:58:47+01:00 Water plays a different role on activation thermodynamic parameters of alcoholysis reaction catalyzed by Lipase in gaseous and organic media Graber, Marianne Bousquet-Dubouch, Marie-Pierre Sousa, Nadine Lamare, Sylvain Legoy, Marie-Dominique LIttoral ENvironnement et Sociétés (LIENSs) La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Génie Protéique et Cellulaire (LGPC) La Rochelle Université (ULR) 2002-10-17 https://hal.science/hal-00329663 https://hal.science/hal-00329663/document https://hal.science/hal-00329663/file/publi2.pdf en eng HAL CCSD Elsevier hal-00329663 https://hal.science/hal-00329663 https://hal.science/hal-00329663/document https://hal.science/hal-00329663/file/publi2.pdf info:eu-repo/semantics/OpenAccess ISSN: 1570-9639 Biochimica et Biophysica Acta Proteins and Proteomics https://hal.science/hal-00329663 Biochimica et Biophysica Acta Proteins and Proteomics, 2002, 1645, pp.56-62 [SDV.BIO]Life Sciences [q-bio]/Biotechnology [INFO.INFO-BT]Computer Science [cs]/Biotechnology info:eu-repo/semantics/article Journal articles 2002 ftunivrochelle 2024-01-23T23:34:42Z International audience The effect of water on the alcoholysis of methyl propionate and n-propanol catalyzed by immobilized Candida antarctica lipase B (CALB) has been compared in a continuous solid/gas reactor and in an organic liquid medium. The enthalpic and entropic contributions of water to the Gibbs free energy of activation in the gas phase were different from the ones in the organic phase, the inverse trends being observed for the variation of both ∃H* and ∃S* with water activity. Different phenomena were identified for their influence on the thermodynamic parameters. When increasing aW, the enhanced flexibility of the enzyme was predominant in the gas phase whereas enzyme-solvent interactions due to an increased polarity of the solvent affected mainly the thermodynamic parameters in the organic phase. The observed variations of ∃G* with water activity were in accordance with kinetics results previously obtained in both reaction media. Article in Journal/Newspaper Antarc* Antarctica HAL - Université de La Rochelle |
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Open Polar |
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HAL - Université de La Rochelle |
op_collection_id |
ftunivrochelle |
language |
English |
topic |
[SDV.BIO]Life Sciences [q-bio]/Biotechnology [INFO.INFO-BT]Computer Science [cs]/Biotechnology |
spellingShingle |
[SDV.BIO]Life Sciences [q-bio]/Biotechnology [INFO.INFO-BT]Computer Science [cs]/Biotechnology Graber, Marianne Bousquet-Dubouch, Marie-Pierre Sousa, Nadine Lamare, Sylvain Legoy, Marie-Dominique Water plays a different role on activation thermodynamic parameters of alcoholysis reaction catalyzed by Lipase in gaseous and organic media |
topic_facet |
[SDV.BIO]Life Sciences [q-bio]/Biotechnology [INFO.INFO-BT]Computer Science [cs]/Biotechnology |
description |
International audience The effect of water on the alcoholysis of methyl propionate and n-propanol catalyzed by immobilized Candida antarctica lipase B (CALB) has been compared in a continuous solid/gas reactor and in an organic liquid medium. The enthalpic and entropic contributions of water to the Gibbs free energy of activation in the gas phase were different from the ones in the organic phase, the inverse trends being observed for the variation of both ∃H* and ∃S* with water activity. Different phenomena were identified for their influence on the thermodynamic parameters. When increasing aW, the enhanced flexibility of the enzyme was predominant in the gas phase whereas enzyme-solvent interactions due to an increased polarity of the solvent affected mainly the thermodynamic parameters in the organic phase. The observed variations of ∃G* with water activity were in accordance with kinetics results previously obtained in both reaction media. |
author2 |
LIttoral ENvironnement et Sociétés (LIENSs) La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Génie Protéique et Cellulaire (LGPC) La Rochelle Université (ULR) |
format |
Article in Journal/Newspaper |
author |
Graber, Marianne Bousquet-Dubouch, Marie-Pierre Sousa, Nadine Lamare, Sylvain Legoy, Marie-Dominique |
author_facet |
Graber, Marianne Bousquet-Dubouch, Marie-Pierre Sousa, Nadine Lamare, Sylvain Legoy, Marie-Dominique |
author_sort |
Graber, Marianne |
title |
Water plays a different role on activation thermodynamic parameters of alcoholysis reaction catalyzed by Lipase in gaseous and organic media |
title_short |
Water plays a different role on activation thermodynamic parameters of alcoholysis reaction catalyzed by Lipase in gaseous and organic media |
title_full |
Water plays a different role on activation thermodynamic parameters of alcoholysis reaction catalyzed by Lipase in gaseous and organic media |
title_fullStr |
Water plays a different role on activation thermodynamic parameters of alcoholysis reaction catalyzed by Lipase in gaseous and organic media |
title_full_unstemmed |
Water plays a different role on activation thermodynamic parameters of alcoholysis reaction catalyzed by Lipase in gaseous and organic media |
title_sort |
water plays a different role on activation thermodynamic parameters of alcoholysis reaction catalyzed by lipase in gaseous and organic media |
publisher |
HAL CCSD |
publishDate |
2002 |
url |
https://hal.science/hal-00329663 https://hal.science/hal-00329663/document https://hal.science/hal-00329663/file/publi2.pdf |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
ISSN: 1570-9639 Biochimica et Biophysica Acta Proteins and Proteomics https://hal.science/hal-00329663 Biochimica et Biophysica Acta Proteins and Proteomics, 2002, 1645, pp.56-62 |
op_relation |
hal-00329663 https://hal.science/hal-00329663 https://hal.science/hal-00329663/document https://hal.science/hal-00329663/file/publi2.pdf |
op_rights |
info:eu-repo/semantics/OpenAccess |
_version_ |
1790594543398158336 |