Comparative proteomics of an extended spectrum β-lactamase producing Escherichia coli strain from the Iberian wolf
The Iberian wolf (Canis lupus signatus) is an endangered species native to the Iberian Peninsula. Due to their predatory and wild nature, these wolves serve as important indicators of environmental contamination by antimicrobial-resistant bacteria. -Lactam antibiotics like cefotaxime are the most co...
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Online Access: | https://investigacion.unirioja.es/documentos/5bbc6a09b750603269e825de https://doi.org/10.1016/J.JPROT.2014.02.033 |
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ftunivriojair:oai:portal.dialnet.es:doc/5bbc6a09b750603269e825de 2023-05-15T15:50:51+02:00 Comparative proteomics of an extended spectrum β-lactamase producing Escherichia coli strain from the Iberian wolf Gonçalves, A. Poeta, P. Monteiro, R. Marinho, C. Silva, N. Guerra, A. Petrucci-Fonseca, F. Rodrigues, J. Torres, C. Vitorino, R. Domingues, P. Igrejas, G. 2014 application/pdf https://investigacion.unirioja.es/documentos/5bbc6a09b750603269e825de https://doi.org/10.1016/J.JPROT.2014.02.033 eng eng info:eu-repo/semantics/altIdentifier/doi/10.1016/J.JPROT.2014.02.033 info:eu-repo/semantics/altIdentifier/wos/WOS:000337878100008 info:eu-repo/semantics/altIdentifier/pissn/1874-3919 Comparative proteomics of an extended spectrum β-lactamase producing Escherichia coli strain from the Iberian wolf, 2014, vol. 104, pág. 80-93 https://investigacion.unirioja.es/documentos/5bbc6a09b750603269e825de doi:10.1016/J.JPROT.2014.02.033 urn:issn:1874-3919 info:eu-repo/semantics/openAccess Antibiotic resistance Escherichia coli Iberian wolf Mass spectrometry Subcellular proteomes info:eu-repo/semantics/article Subtype: Article info:eu-repo/semantics/publishedVersion 2014 ftunivriojair https://doi.org/10.1016/J.JPROT.2014.02.033 2022-09-19T07:59:56Z The Iberian wolf (Canis lupus signatus) is an endangered species native to the Iberian Peninsula. Due to their predatory and wild nature, these wolves serve as important indicators of environmental contamination by antimicrobial-resistant bacteria. -Lactam antibiotics like cefotaxime are the most commonly used antibacterial agents. Bacterial resistance to these antibiotics occurs predominantly through enzymatic inactivation by extended-spectrum beta-lactamases. Escherichia coli strain WA57, isolated from Iberian wolf feces, is a cefotaxime-resistant strain that produces extended-spectrum beta-lactamases. In this study, using 2D-GE combined with MS and bioinformatics, we report significant differences in the abundance of 40 protein spots (p < 0.01) from the extracellular, periplasmic, cytoplasmic, and membrane sub-proteomes and the whole-cell proteome of WA57 exposed and non-exposed to cefotaxime. A total of 315 protein spots were collected for protein identification. The comparative proteomics presented gives an overview of the complex changes in expression and metabolism that occur when WA57 is stressed with cefotaxime. Abundance of chaperone, porin and export proteins is particularly affected showing that the stress response and transport functions might directly influence the antibiotic resistance of this strain. Biological significance: This study highlights the importance of proteomics in detecting protein expression changes in bacterial strains exposed to stress such as that caused by cefotaxime. This approach might help us understand which pathways form barriers for antibiotics. This article is part of a Special Issue entitled: Environmental and structural proteomics. © 2014 Elsevier B.V. All rights reserved. Article in Journal/Newspaper Canis lupus Portal de la Investigación Universidad de La Rioja Journal of Proteomics 104 80 93 |
institution |
Open Polar |
collection |
Portal de la Investigación Universidad de La Rioja |
op_collection_id |
ftunivriojair |
language |
English |
topic |
Antibiotic resistance Escherichia coli Iberian wolf Mass spectrometry Subcellular proteomes |
spellingShingle |
Antibiotic resistance Escherichia coli Iberian wolf Mass spectrometry Subcellular proteomes Gonçalves, A. Poeta, P. Monteiro, R. Marinho, C. Silva, N. Guerra, A. Petrucci-Fonseca, F. Rodrigues, J. Torres, C. Vitorino, R. Domingues, P. Igrejas, G. Comparative proteomics of an extended spectrum β-lactamase producing Escherichia coli strain from the Iberian wolf |
topic_facet |
Antibiotic resistance Escherichia coli Iberian wolf Mass spectrometry Subcellular proteomes |
description |
The Iberian wolf (Canis lupus signatus) is an endangered species native to the Iberian Peninsula. Due to their predatory and wild nature, these wolves serve as important indicators of environmental contamination by antimicrobial-resistant bacteria. -Lactam antibiotics like cefotaxime are the most commonly used antibacterial agents. Bacterial resistance to these antibiotics occurs predominantly through enzymatic inactivation by extended-spectrum beta-lactamases. Escherichia coli strain WA57, isolated from Iberian wolf feces, is a cefotaxime-resistant strain that produces extended-spectrum beta-lactamases. In this study, using 2D-GE combined with MS and bioinformatics, we report significant differences in the abundance of 40 protein spots (p < 0.01) from the extracellular, periplasmic, cytoplasmic, and membrane sub-proteomes and the whole-cell proteome of WA57 exposed and non-exposed to cefotaxime. A total of 315 protein spots were collected for protein identification. The comparative proteomics presented gives an overview of the complex changes in expression and metabolism that occur when WA57 is stressed with cefotaxime. Abundance of chaperone, porin and export proteins is particularly affected showing that the stress response and transport functions might directly influence the antibiotic resistance of this strain. Biological significance: This study highlights the importance of proteomics in detecting protein expression changes in bacterial strains exposed to stress such as that caused by cefotaxime. This approach might help us understand which pathways form barriers for antibiotics. This article is part of a Special Issue entitled: Environmental and structural proteomics. © 2014 Elsevier B.V. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Gonçalves, A. Poeta, P. Monteiro, R. Marinho, C. Silva, N. Guerra, A. Petrucci-Fonseca, F. Rodrigues, J. Torres, C. Vitorino, R. Domingues, P. Igrejas, G. |
author_facet |
Gonçalves, A. Poeta, P. Monteiro, R. Marinho, C. Silva, N. Guerra, A. Petrucci-Fonseca, F. Rodrigues, J. Torres, C. Vitorino, R. Domingues, P. Igrejas, G. |
author_sort |
Gonçalves, A. |
title |
Comparative proteomics of an extended spectrum β-lactamase producing Escherichia coli strain from the Iberian wolf |
title_short |
Comparative proteomics of an extended spectrum β-lactamase producing Escherichia coli strain from the Iberian wolf |
title_full |
Comparative proteomics of an extended spectrum β-lactamase producing Escherichia coli strain from the Iberian wolf |
title_fullStr |
Comparative proteomics of an extended spectrum β-lactamase producing Escherichia coli strain from the Iberian wolf |
title_full_unstemmed |
Comparative proteomics of an extended spectrum β-lactamase producing Escherichia coli strain from the Iberian wolf |
title_sort |
comparative proteomics of an extended spectrum β-lactamase producing escherichia coli strain from the iberian wolf |
publishDate |
2014 |
url |
https://investigacion.unirioja.es/documentos/5bbc6a09b750603269e825de https://doi.org/10.1016/J.JPROT.2014.02.033 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/J.JPROT.2014.02.033 info:eu-repo/semantics/altIdentifier/wos/WOS:000337878100008 info:eu-repo/semantics/altIdentifier/pissn/1874-3919 Comparative proteomics of an extended spectrum β-lactamase producing Escherichia coli strain from the Iberian wolf, 2014, vol. 104, pág. 80-93 https://investigacion.unirioja.es/documentos/5bbc6a09b750603269e825de doi:10.1016/J.JPROT.2014.02.033 urn:issn:1874-3919 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/J.JPROT.2014.02.033 |
container_title |
Journal of Proteomics |
container_volume |
104 |
container_start_page |
80 |
op_container_end_page |
93 |
_version_ |
1766385876836810752 |