Medium Term Conservation of Several Carnation Accessions Via in Vitro Culture
Sufficient genetic diversity is important in carnation breeding program. In vivo conservation of carnation germplasmis considered inefficient due to some technical and economical aspects. In vitro conservation was then, expectedto overcome the limitation of in vivo method. The research was conducted...
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2012
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ftunivriauojs:oai:ejournal.unri.ac.id:article/175 2023-05-15T18:40:26+02:00 Medium Term Conservation of Several Carnation Accessions Via in Vitro Culture Budiarto, Kurniawan Marwoto, Budi 2012-11-21 application/pdf https://ejournal.unri.ac.id/index.php/JN/article/view/175 https://doi.org/10.31258/jni.13.02.%p eng eng Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau https://ejournal.unri.ac.id/index.php/JN/article/view/175/169 Copyright (c) 2012 Kurniawan Budiarto, Budi Marwoto http://creativecommons.org/licenses/by/4.0 CC-BY Jurnal Natur Indonesia; Vol 13, No 02 (2011); 174-177 2503-0345 1410-9379 accessions Carnation (Dianthus carryophyllus) in vitro conservation low temperature storage media info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article 2012 ftunivriauojs https://doi.org/10.31258/jni.13.02.%p 2019-07-09T15:43:20Z Sufficient genetic diversity is important in carnation breeding program. In vivo conservation of carnation germplasmis considered inefficient due to some technical and economical aspects. In vitro conservation was then, expectedto overcome the limitation of in vivo method. The research was conducted to find out the proper media for medium-term in vitro conservation of several carnation accessions in low temperature storage. A complete factorialexperiment with 25 replications was designed to accomplish the combination of two factors. The first factor wassix commercial carnation cultivars, namely Pink Maladi, Orange Triumph, Opera, Tundra, Yellow Liberty and PradoReffit. The second factor was the conservation media i.e. 1⁄2MS + DMSO 3% and 1⁄2MS + 3% DMSO + 3% sucrose andcontrol (MS 0+3% sucrose). The results showed that in vitro conservation of carnation in low temperature weresuccessfully conducted using 1⁄2MS+3% DMSO and 1⁄2MS+3% DMSO+3% sucrose without significant variation in allaccessions tested up to 10 and 12 months respectively. The increase of death plantlets, however, was detected onthe media of 1⁄2MS+3% DMSO after 6 months storage with significant decrease in viability hereafter. The existenceof sucrose in DMSO media induced root formation and plantlet resistance to low temperature storage. Article in Journal/Newspaper Tundra E-Journal of Riau University |
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Open Polar |
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E-Journal of Riau University |
op_collection_id |
ftunivriauojs |
language |
English |
topic |
accessions Carnation (Dianthus carryophyllus) in vitro conservation low temperature storage media |
spellingShingle |
accessions Carnation (Dianthus carryophyllus) in vitro conservation low temperature storage media Budiarto, Kurniawan Marwoto, Budi Medium Term Conservation of Several Carnation Accessions Via in Vitro Culture |
topic_facet |
accessions Carnation (Dianthus carryophyllus) in vitro conservation low temperature storage media |
description |
Sufficient genetic diversity is important in carnation breeding program. In vivo conservation of carnation germplasmis considered inefficient due to some technical and economical aspects. In vitro conservation was then, expectedto overcome the limitation of in vivo method. The research was conducted to find out the proper media for medium-term in vitro conservation of several carnation accessions in low temperature storage. A complete factorialexperiment with 25 replications was designed to accomplish the combination of two factors. The first factor wassix commercial carnation cultivars, namely Pink Maladi, Orange Triumph, Opera, Tundra, Yellow Liberty and PradoReffit. The second factor was the conservation media i.e. 1⁄2MS + DMSO 3% and 1⁄2MS + 3% DMSO + 3% sucrose andcontrol (MS 0+3% sucrose). The results showed that in vitro conservation of carnation in low temperature weresuccessfully conducted using 1⁄2MS+3% DMSO and 1⁄2MS+3% DMSO+3% sucrose without significant variation in allaccessions tested up to 10 and 12 months respectively. The increase of death plantlets, however, was detected onthe media of 1⁄2MS+3% DMSO after 6 months storage with significant decrease in viability hereafter. The existenceof sucrose in DMSO media induced root formation and plantlet resistance to low temperature storage. |
format |
Article in Journal/Newspaper |
author |
Budiarto, Kurniawan Marwoto, Budi |
author_facet |
Budiarto, Kurniawan Marwoto, Budi |
author_sort |
Budiarto, Kurniawan |
title |
Medium Term Conservation of Several Carnation Accessions Via in Vitro Culture |
title_short |
Medium Term Conservation of Several Carnation Accessions Via in Vitro Culture |
title_full |
Medium Term Conservation of Several Carnation Accessions Via in Vitro Culture |
title_fullStr |
Medium Term Conservation of Several Carnation Accessions Via in Vitro Culture |
title_full_unstemmed |
Medium Term Conservation of Several Carnation Accessions Via in Vitro Culture |
title_sort |
medium term conservation of several carnation accessions via in vitro culture |
publisher |
Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau |
publishDate |
2012 |
url |
https://ejournal.unri.ac.id/index.php/JN/article/view/175 https://doi.org/10.31258/jni.13.02.%p |
genre |
Tundra |
genre_facet |
Tundra |
op_source |
Jurnal Natur Indonesia; Vol 13, No 02 (2011); 174-177 2503-0345 1410-9379 |
op_relation |
https://ejournal.unri.ac.id/index.php/JN/article/view/175/169 |
op_rights |
Copyright (c) 2012 Kurniawan Budiarto, Budi Marwoto http://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.31258/jni.13.02.%p |
_version_ |
1766229783840030720 |