DECIPHERING BIOGENIC SILICA PRODUCTION TRENDS IN PICOPLANKTON OF THE SUBARCTIC NORTH PACIFIC USING NOVEL DNA BARCODING METHODS
The Silica cycle and the biological pump in our global oceans are intimately related. As a part of the NASA Export Processes in the Ocean from Remote Sensing (EXPORTS) our work aims to explore the community composition of picoplankton in the 0.2-5µm size fraction and their contributions to the silic...
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ftunivrhodeislan:oai:digitalcommons.uri.edu:theses-3226 2023-07-30T04:07:09+02:00 DECIPHERING BIOGENIC SILICA PRODUCTION TRENDS IN PICOPLANKTON OF THE SUBARCTIC NORTH PACIFIC USING NOVEL DNA BARCODING METHODS Presley, Andrew 2022-01-01T08:00:00Z application/pdf https://digitalcommons.uri.edu/theses/2253 https://doi.org/10.23860/thesis-presley-andrew-2022 https://digitalcommons.uri.edu/context/theses/article/3226/viewcontent/Presley_uri_0186M_12951.pdf unknown DigitalCommons@URI https://digitalcommons.uri.edu/theses/2253 doi:10.23860/thesis-presley-andrew-2022 https://digitalcommons.uri.edu/context/theses/article/3226/viewcontent/Presley_uri_0186M_12951.pdf Open Access Master's Theses text 2022 ftunivrhodeislan https://doi.org/10.23860/thesis-presley-andrew-2022 2023-07-17T18:49:25Z The Silica cycle and the biological pump in our global oceans are intimately related. As a part of the NASA Export Processes in the Ocean from Remote Sensing (EXPORTS) our work aims to explore the community composition of picoplankton in the 0.2-5µm size fraction and their contributions to the silica cycle using next generation MiSeq sequencing and analysis. Recently published data from collaborators within the EXPORTS project have observed high biogenic silica production in the picoplankton, indicating that organisms in this size fraction are contributing notably to the silica cycle in the North Pacific, though it remains unclear exactly what organisms are responsible. During our analysis we have also pioneered the use of an internal DNA reference within the picoplankton for the calculation of absolute sequence abundance, as well as the reduction of sequencing, PCR and DNA extraction variability in community analyses. By pioneering this method in our analysis, we hope to create an example for future labs both within the EXPORTS group as well as the Oceanographic community as a whole to utilize in their analyses going forwards to better allow collaboration and comparison of community analysis work. Text Subarctic University of Rhode Island: DigitalCommons@URI Pacific |
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Open Polar |
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University of Rhode Island: DigitalCommons@URI |
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ftunivrhodeislan |
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description |
The Silica cycle and the biological pump in our global oceans are intimately related. As a part of the NASA Export Processes in the Ocean from Remote Sensing (EXPORTS) our work aims to explore the community composition of picoplankton in the 0.2-5µm size fraction and their contributions to the silica cycle using next generation MiSeq sequencing and analysis. Recently published data from collaborators within the EXPORTS project have observed high biogenic silica production in the picoplankton, indicating that organisms in this size fraction are contributing notably to the silica cycle in the North Pacific, though it remains unclear exactly what organisms are responsible. During our analysis we have also pioneered the use of an internal DNA reference within the picoplankton for the calculation of absolute sequence abundance, as well as the reduction of sequencing, PCR and DNA extraction variability in community analyses. By pioneering this method in our analysis, we hope to create an example for future labs both within the EXPORTS group as well as the Oceanographic community as a whole to utilize in their analyses going forwards to better allow collaboration and comparison of community analysis work. |
format |
Text |
author |
Presley, Andrew |
spellingShingle |
Presley, Andrew DECIPHERING BIOGENIC SILICA PRODUCTION TRENDS IN PICOPLANKTON OF THE SUBARCTIC NORTH PACIFIC USING NOVEL DNA BARCODING METHODS |
author_facet |
Presley, Andrew |
author_sort |
Presley, Andrew |
title |
DECIPHERING BIOGENIC SILICA PRODUCTION TRENDS IN PICOPLANKTON OF THE SUBARCTIC NORTH PACIFIC USING NOVEL DNA BARCODING METHODS |
title_short |
DECIPHERING BIOGENIC SILICA PRODUCTION TRENDS IN PICOPLANKTON OF THE SUBARCTIC NORTH PACIFIC USING NOVEL DNA BARCODING METHODS |
title_full |
DECIPHERING BIOGENIC SILICA PRODUCTION TRENDS IN PICOPLANKTON OF THE SUBARCTIC NORTH PACIFIC USING NOVEL DNA BARCODING METHODS |
title_fullStr |
DECIPHERING BIOGENIC SILICA PRODUCTION TRENDS IN PICOPLANKTON OF THE SUBARCTIC NORTH PACIFIC USING NOVEL DNA BARCODING METHODS |
title_full_unstemmed |
DECIPHERING BIOGENIC SILICA PRODUCTION TRENDS IN PICOPLANKTON OF THE SUBARCTIC NORTH PACIFIC USING NOVEL DNA BARCODING METHODS |
title_sort |
deciphering biogenic silica production trends in picoplankton of the subarctic north pacific using novel dna barcoding methods |
publisher |
DigitalCommons@URI |
publishDate |
2022 |
url |
https://digitalcommons.uri.edu/theses/2253 https://doi.org/10.23860/thesis-presley-andrew-2022 https://digitalcommons.uri.edu/context/theses/article/3226/viewcontent/Presley_uri_0186M_12951.pdf |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Subarctic |
genre_facet |
Subarctic |
op_source |
Open Access Master's Theses |
op_relation |
https://digitalcommons.uri.edu/theses/2253 doi:10.23860/thesis-presley-andrew-2022 https://digitalcommons.uri.edu/context/theses/article/3226/viewcontent/Presley_uri_0186M_12951.pdf |
op_doi |
https://doi.org/10.23860/thesis-presley-andrew-2022 |
_version_ |
1772820292950818816 |