Hydrogen Utilization Potential in Subsurface Sediments
Subsurface microbial communities undertake many terminal electron-accepting processes, often simultaneously. Using a tritium-based assay, we measured the potential hydrogen oxidation catalyzed by hydrogenase enzymes in several subsurface sedimentary environments (Lake Van, Barents Sea, Equatorial Pa...
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ftunivrhodeislan:oai:digitalcommons.uri.edu:gsofacpubs-1154 2023-07-30T04:02:35+02:00 Hydrogen Utilization Potential in Subsurface Sediments Adhikari, Rishi R. Glombitza, Clemens Nickel, Julia C. Anderson, Chloe H. Dunlea, Ann G. Spivack, Arthur J. Murray, Richard W. D'Hondt, Steven Kallmeyer, Jens 2016-01-26T08:00:00Z application/pdf https://digitalcommons.uri.edu/gsofacpubs/148 https://doi.org/10.3389/fmicb.2016.00008 https://digitalcommons.uri.edu/context/gsofacpubs/article/1154/viewcontent/fmicb_07_00008_2.pdf unknown DigitalCommons@URI https://digitalcommons.uri.edu/gsofacpubs/148 doi:10.3389/fmicb.2016.00008 https://digitalcommons.uri.edu/context/gsofacpubs/article/1154/viewcontent/fmicb_07_00008_2.pdf http://creativecommons.org/licenses/by/4.0/ Graduate School of Oceanography Faculty Publications text 2016 ftunivrhodeislan https://doi.org/10.3389/fmicb.2016.00008 2023-07-17T18:55:01Z Subsurface microbial communities undertake many terminal electron-accepting processes, often simultaneously. Using a tritium-based assay, we measured the potential hydrogen oxidation catalyzed by hydrogenase enzymes in several subsurface sedimentary environments (Lake Van, Barents Sea, Equatorial Pacific, and Gulf of Mexico) with different predominant electron-acceptors. Hydrogenases constitute a diverse family of enzymes expressed by microorganisms that utilize molecular hydrogen as a metabolic substrate, product, or intermediate. The assay reveals the potential for utilizing molecular hydrogen and allows qualitative detection of microbial activity irrespective of the predominant electron-accepting process. Because the method only requires samples frozen immediately after recovery, the assay can be used for identifying microbial activity in subsurface ecosystems without the need to preserve live material. We measured potential hydrogen oxidation rates in all samples from multiple depths at several sites that collectively span a wide range of environmental conditions and biogeochemical zones. Potential activity normalized to total cell abundance ranges over five orders of magnitude and varies, dependent upon the predominant terminal electron acceptor. Lowest per-cell potential rates characterize the zone of nitrate reduction and highest per-cell potential rates occur in the methanogenic zone. Possible reasons for this relationship to predominant electron acceptor include (i) increasing importance of fermentation in successively deeper biogeochemical zones and (ii) adaptation of H2ases to successively higher concentrations of H2 in successively deeper zones. Text Barents Sea University of Rhode Island: DigitalCommons@URI Barents Sea Pacific Frontiers in Microbiology 7 |
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Open Polar |
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University of Rhode Island: DigitalCommons@URI |
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ftunivrhodeislan |
language |
unknown |
description |
Subsurface microbial communities undertake many terminal electron-accepting processes, often simultaneously. Using a tritium-based assay, we measured the potential hydrogen oxidation catalyzed by hydrogenase enzymes in several subsurface sedimentary environments (Lake Van, Barents Sea, Equatorial Pacific, and Gulf of Mexico) with different predominant electron-acceptors. Hydrogenases constitute a diverse family of enzymes expressed by microorganisms that utilize molecular hydrogen as a metabolic substrate, product, or intermediate. The assay reveals the potential for utilizing molecular hydrogen and allows qualitative detection of microbial activity irrespective of the predominant electron-accepting process. Because the method only requires samples frozen immediately after recovery, the assay can be used for identifying microbial activity in subsurface ecosystems without the need to preserve live material. We measured potential hydrogen oxidation rates in all samples from multiple depths at several sites that collectively span a wide range of environmental conditions and biogeochemical zones. Potential activity normalized to total cell abundance ranges over five orders of magnitude and varies, dependent upon the predominant terminal electron acceptor. Lowest per-cell potential rates characterize the zone of nitrate reduction and highest per-cell potential rates occur in the methanogenic zone. Possible reasons for this relationship to predominant electron acceptor include (i) increasing importance of fermentation in successively deeper biogeochemical zones and (ii) adaptation of H2ases to successively higher concentrations of H2 in successively deeper zones. |
format |
Text |
author |
Adhikari, Rishi R. Glombitza, Clemens Nickel, Julia C. Anderson, Chloe H. Dunlea, Ann G. Spivack, Arthur J. Murray, Richard W. D'Hondt, Steven Kallmeyer, Jens |
spellingShingle |
Adhikari, Rishi R. Glombitza, Clemens Nickel, Julia C. Anderson, Chloe H. Dunlea, Ann G. Spivack, Arthur J. Murray, Richard W. D'Hondt, Steven Kallmeyer, Jens Hydrogen Utilization Potential in Subsurface Sediments |
author_facet |
Adhikari, Rishi R. Glombitza, Clemens Nickel, Julia C. Anderson, Chloe H. Dunlea, Ann G. Spivack, Arthur J. Murray, Richard W. D'Hondt, Steven Kallmeyer, Jens |
author_sort |
Adhikari, Rishi R. |
title |
Hydrogen Utilization Potential in Subsurface Sediments |
title_short |
Hydrogen Utilization Potential in Subsurface Sediments |
title_full |
Hydrogen Utilization Potential in Subsurface Sediments |
title_fullStr |
Hydrogen Utilization Potential in Subsurface Sediments |
title_full_unstemmed |
Hydrogen Utilization Potential in Subsurface Sediments |
title_sort |
hydrogen utilization potential in subsurface sediments |
publisher |
DigitalCommons@URI |
publishDate |
2016 |
url |
https://digitalcommons.uri.edu/gsofacpubs/148 https://doi.org/10.3389/fmicb.2016.00008 https://digitalcommons.uri.edu/context/gsofacpubs/article/1154/viewcontent/fmicb_07_00008_2.pdf |
geographic |
Barents Sea Pacific |
geographic_facet |
Barents Sea Pacific |
genre |
Barents Sea |
genre_facet |
Barents Sea |
op_source |
Graduate School of Oceanography Faculty Publications |
op_relation |
https://digitalcommons.uri.edu/gsofacpubs/148 doi:10.3389/fmicb.2016.00008 https://digitalcommons.uri.edu/context/gsofacpubs/article/1154/viewcontent/fmicb_07_00008_2.pdf |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fmicb.2016.00008 |
container_title |
Frontiers in Microbiology |
container_volume |
7 |
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1772813396034453504 |