Biodiesel Production (FAEEs) by Heterogeneous Combi-Lipase Biocatalysts Using Wet Extracted Lipids from Microalgae
Funding: The authors acknowledge the support from INSPIRA1 project (S2013/ABI-2783) financed by Comunidad de Madrid and co-financed by FEDER “A way of making Europe”; and BIOHIDROALGA project (ENE2017-83696-R) financed by Ministerio de Ciencia, Innovación y Universidades and co-financed by FEDER “A...
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Online Access: | https://hdl.handle.net/10115/27537 https://doi.org/10.3390/catal9030296 |
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ftunivreyjc:oai:eciencia.urjc.es:10115/27537 2024-01-21T09:59:27+01:00 Biodiesel Production (FAEEs) by Heterogeneous Combi-Lipase Biocatalysts Using Wet Extracted Lipids from Microalgae Sánchez-Bayo, Alejandra Morales, Victoria Rodríguez, Rosalía Vicente, Gemma Bautista, Luis Fernando 2019 https://hdl.handle.net/10115/27537 https://doi.org/10.3390/catal9030296 eng eng MDPI Catalysts 2019, 9, 296; doi:10.3390/catal9030296 2073-4344 https://hdl.handle.net/10115/27537 doi:10.3390/catal9030296 Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess FAEEs biodiesel mixed biocatalysts lipases microalgae info:eu-repo/semantics/article 2019 ftunivreyjc https://doi.org/10.3390/catal9030296 2023-12-27T00:13:03Z Funding: The authors acknowledge the support from INSPIRA1 project (S2013/ABI-2783) financed by Comunidad de Madrid and co-financed by FEDER “A way of making Europe”; and BIOHIDROALGA project (ENE2017-83696-R) financed by Ministerio de Ciencia, Innovación y Universidades and co-financed by FEDER “A way of making Europe” The production of fatty acids ethyl esters (FAEEs) to be used as biodiesel from oleaginous microalgae shows great opportunities as an attractive source for the production of renewable fuels without competing with human food. To ensure the economic viability and environmental sustainability of the microbial biomass as a raw material, the integration of its production and transformation into the biorefinery concept is required. In the present work, lipids from wet Isochrysis galbana microalga were extracted with ethyl acetate with and without drying the microalgal biomass (dry and wet extraction method, respectively). Then, FAEEs were produced by lipase-catalyzed transesterification and esterification of the extracted lipids with ethanol using lipase B from Candida antarctica (CALB) and Pseudomonas cepacia (PC) lipase supported on SBA-15 mesoporous silica functionalized with amino groups. The conversion to FAEEs with CALB (97 and 85.5 mol% for dry and wet extraction, respectively) and PC (91 and 87 mol%) biocatalysts reached higher values than those obtained with commercial Novozym 435 (75 and 69.5 mol%). Due to the heterogeneous nature of the composition of microalgae lipids, mixtures with different CALB:PC biocatalyst ratio were used to improve conversion of wet-extracted lipids. The results showed that a 25:75 combi-lipase produced a significantly higher conversion to FAEEs (97.2 mol%) than those produced by each biocatalyst independently from wet-extracted lipids and similar ones than those obtained by each lipase from the dry extraction method. Therefore, that optimized combi-lipase biocatalyst, along with achieving the highest conversion to FAEEs, would allow improving viability of a ... Article in Journal/Newspaper Antarc* Antarctica Universidad Rey Juan Carlos, Madrid: Archivo Abierto Institucional Catalysts 9 3 296 |
institution |
Open Polar |
collection |
Universidad Rey Juan Carlos, Madrid: Archivo Abierto Institucional |
op_collection_id |
ftunivreyjc |
language |
English |
topic |
FAEEs biodiesel mixed biocatalysts lipases microalgae |
spellingShingle |
FAEEs biodiesel mixed biocatalysts lipases microalgae Sánchez-Bayo, Alejandra Morales, Victoria Rodríguez, Rosalía Vicente, Gemma Bautista, Luis Fernando Biodiesel Production (FAEEs) by Heterogeneous Combi-Lipase Biocatalysts Using Wet Extracted Lipids from Microalgae |
topic_facet |
FAEEs biodiesel mixed biocatalysts lipases microalgae |
description |
Funding: The authors acknowledge the support from INSPIRA1 project (S2013/ABI-2783) financed by Comunidad de Madrid and co-financed by FEDER “A way of making Europe”; and BIOHIDROALGA project (ENE2017-83696-R) financed by Ministerio de Ciencia, Innovación y Universidades and co-financed by FEDER “A way of making Europe” The production of fatty acids ethyl esters (FAEEs) to be used as biodiesel from oleaginous microalgae shows great opportunities as an attractive source for the production of renewable fuels without competing with human food. To ensure the economic viability and environmental sustainability of the microbial biomass as a raw material, the integration of its production and transformation into the biorefinery concept is required. In the present work, lipids from wet Isochrysis galbana microalga were extracted with ethyl acetate with and without drying the microalgal biomass (dry and wet extraction method, respectively). Then, FAEEs were produced by lipase-catalyzed transesterification and esterification of the extracted lipids with ethanol using lipase B from Candida antarctica (CALB) and Pseudomonas cepacia (PC) lipase supported on SBA-15 mesoporous silica functionalized with amino groups. The conversion to FAEEs with CALB (97 and 85.5 mol% for dry and wet extraction, respectively) and PC (91 and 87 mol%) biocatalysts reached higher values than those obtained with commercial Novozym 435 (75 and 69.5 mol%). Due to the heterogeneous nature of the composition of microalgae lipids, mixtures with different CALB:PC biocatalyst ratio were used to improve conversion of wet-extracted lipids. The results showed that a 25:75 combi-lipase produced a significantly higher conversion to FAEEs (97.2 mol%) than those produced by each biocatalyst independently from wet-extracted lipids and similar ones than those obtained by each lipase from the dry extraction method. Therefore, that optimized combi-lipase biocatalyst, along with achieving the highest conversion to FAEEs, would allow improving viability of a ... |
format |
Article in Journal/Newspaper |
author |
Sánchez-Bayo, Alejandra Morales, Victoria Rodríguez, Rosalía Vicente, Gemma Bautista, Luis Fernando |
author_facet |
Sánchez-Bayo, Alejandra Morales, Victoria Rodríguez, Rosalía Vicente, Gemma Bautista, Luis Fernando |
author_sort |
Sánchez-Bayo, Alejandra |
title |
Biodiesel Production (FAEEs) by Heterogeneous Combi-Lipase Biocatalysts Using Wet Extracted Lipids from Microalgae |
title_short |
Biodiesel Production (FAEEs) by Heterogeneous Combi-Lipase Biocatalysts Using Wet Extracted Lipids from Microalgae |
title_full |
Biodiesel Production (FAEEs) by Heterogeneous Combi-Lipase Biocatalysts Using Wet Extracted Lipids from Microalgae |
title_fullStr |
Biodiesel Production (FAEEs) by Heterogeneous Combi-Lipase Biocatalysts Using Wet Extracted Lipids from Microalgae |
title_full_unstemmed |
Biodiesel Production (FAEEs) by Heterogeneous Combi-Lipase Biocatalysts Using Wet Extracted Lipids from Microalgae |
title_sort |
biodiesel production (faees) by heterogeneous combi-lipase biocatalysts using wet extracted lipids from microalgae |
publisher |
MDPI |
publishDate |
2019 |
url |
https://hdl.handle.net/10115/27537 https://doi.org/10.3390/catal9030296 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
Catalysts 2019, 9, 296; doi:10.3390/catal9030296 2073-4344 https://hdl.handle.net/10115/27537 doi:10.3390/catal9030296 |
op_rights |
Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3390/catal9030296 |
container_title |
Catalysts |
container_volume |
9 |
container_issue |
3 |
container_start_page |
296 |
_version_ |
1788696110598979584 |